Detailed information of effector
Summary
| Effector ID | EFF00086  |
| Effector type | Tse |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00070 (Type i1) |
| Strain | Burkholderia mallei ATCC 23344 |
| Replicon | chromosome |
| Sequence | Protein sequence (408 a.a.); Nucleotide sequence (1224 bp) |
| Description | putative deubiquitinase |
| Reference | [1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434 [2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BMA_RS19535 |
| Coordinates (Strand) | 736735..737958 (-) |
| NCBI ID | 77358950 |
| RefSeq | NC_006349 |
| Uniprot ID | A0A0H2XF55_BURMA |
| KEGG ID | bma:BMAA0729 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Big_5 | PF13205.8 | 4.2e-09 | 110..208 |
| UCH | PF00443.31 | 5.9e-05 | 355..384 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-407 | MRSPQRGGAALALAVASLAGCGGGDSGEPAPREFAPPTVQLAYPTQPNSPVAPAPTAHVSSGHTPPATAPAAMPTASPTATPTASPTATAAASPSAPSTATPNAPPAAPPAVVATRVPPTHAALRRPTIELEFDRAIEPGSVPHIVLRADDGTSVAVGPLSWLSDRRIAFAPRKPLKSNSRYEIMVPAGIRSTTGERSAHPLTSSFDTAPVTPPRGLPNLDGASCFINTALQLAVHSSALDDILSNEAVPPAVRTLLEDYDAASADALDAQLAAAVAALRATPEVPDSGPGQTLEVMQALRMPLYDTSSANNATNNADAIRHAPPNTKAFFLNSYPPLSYADLPNHDRLVAFDYSTGGHYVAYVKRDGIWYRIDDAQVSAVNEQDLLALPAFNPANGSVSIEIAIYR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9986 | pos: 20-21. LAG-CG. | MRSPQRGGAALALAVASLAG |
Protein sequence: 408 a.a. .
>EFF00086 NC_006349:c737958-736735 Type VI Secretion System Effector (Tse) [Burkholderia mallei ATCC 23344]
MRSPQRGGAALALAVASLAGCGGGDSGEPAPREFAPPTVQLAYPTQPNSPVAPAPTAHVSSGHTPPATAPAAMPTASPTA
TPTASPTATAAASPSAPSTATPNAPPAAPPAVVATRVPPTHAALRRPTIELEFDRAIEPGSVPHIVLRADDGTSVAVGPL
SWLSDRRIAFAPRKPLKSNSRYEIMVPAGIRSTTGERSAHPLTSSFDTAPVTPPRGLPNLDGASCFINTALQLAVHSSAL
DDILSNEAVPPAVRTLLEDYDAASADALDAQLAAAVAALRATPEVPDSGPGQTLEVMQALRMPLYDTSSANNATNNADAI
RHAPPNTKAFFLNSYPPLSYADLPNHDRLVAFDYSTGGHYVAYVKRDGIWYRIDDAQVSAVNEQDLLALPAFNPANGSVS
IEIAIYR*
MRSPQRGGAALALAVASLAGCGGGDSGEPAPREFAPPTVQLAYPTQPNSPVAPAPTAHVSSGHTPPATAPAAMPTASPTA
TPTASPTATAAASPSAPSTATPNAPPAAPPAVVATRVPPTHAALRRPTIELEFDRAIEPGSVPHIVLRADDGTSVAVGPL
SWLSDRRIAFAPRKPLKSNSRYEIMVPAGIRSTTGERSAHPLTSSFDTAPVTPPRGLPNLDGASCFINTALQLAVHSSAL
DDILSNEAVPPAVRTLLEDYDAASADALDAQLAAAVAALRATPEVPDSGPGQTLEVMQALRMPLYDTSSANNATNNADAI
RHAPPNTKAFFLNSYPPLSYADLPNHDRLVAFDYSTGGHYVAYVKRDGIWYRIDDAQVSAVNEQDLLALPAFNPANGSVS
IEIAIYR*
Nucleotide sequence: 1224 bp .
>EFF00086 NC_006349:c737958-736735 Type VI Secretion System Effector (Tse) [Burkholderia mallei ATCC 23344]
ATGCGCTCGCCCCAACGCGGCGGCGCGGCACTGGCGCTGGCCGTCGCCTCGCTCGCCGGATGCGGGGGCGGCGATTCGGG
CGAACCCGCTCCACGCGAATTCGCGCCCCCGACAGTGCAACTCGCCTACCCGACGCAACCGAACTCGCCCGTCGCACCAG
CGCCCACCGCGCACGTATCGAGCGGACACACGCCTCCGGCCACGGCGCCCGCCGCGATGCCCACCGCTTCACCCACCGCG
ACGCCTACCGCTTCACCCACCGCGACGGCTGCCGCTTCGCCCTCCGCGCCATCCACTGCGACGCCCAACGCCCCGCCCGC
CGCTCCGCCGGCCGTCGTCGCCACCCGCGTCCCGCCGACGCACGCGGCGTTGCGCCGCCCGACGATCGAACTCGAATTCG
ATCGCGCGATCGAGCCGGGTTCAGTCCCACACATCGTGCTGCGCGCCGACGATGGCACGAGCGTCGCCGTCGGCCCGTTG
TCGTGGCTGAGCGATCGCCGGATCGCGTTCGCGCCGCGCAAACCGCTCAAGTCGAACAGCCGCTACGAAATCATGGTGCC
CGCCGGCATCAGGAGCACCACGGGCGAACGGTCGGCCCATCCGCTAACGAGCAGCTTCGATACCGCGCCCGTTACGCCGC
CGCGCGGCCTGCCCAATCTCGACGGCGCCTCGTGCTTCATCAACACGGCGCTGCAATTGGCGGTTCACTCGTCGGCGCTC
GACGACATTCTGTCGAACGAAGCCGTCCCGCCCGCCGTCCGCACGCTGCTCGAAGACTACGACGCCGCATCGGCTGATGC
ACTCGACGCGCAGTTGGCCGCCGCGGTCGCCGCGCTGCGCGCCACGCCGGAGGTCCCGGACAGCGGGCCGGGACAAACGC
TGGAAGTGATGCAAGCGTTGCGGATGCCGTTATATGACACGAGCAGCGCGAACAACGCAACGAACAACGCCGACGCCATA
CGTCATGCGCCGCCCAACACCAAGGCGTTCTTTCTGAACTCCTATCCACCGCTTTCCTACGCGGATCTGCCGAACCACGA
CCGGCTCGTCGCGTTCGACTACAGCACGGGCGGTCACTATGTCGCTTATGTGAAGCGGGATGGAATCTGGTATCGAATCG
ACGATGCCCAGGTCAGCGCCGTCAACGAACAGGACTTGCTTGCCCTGCCGGCGTTCAACCCCGCTAACGGCAGCGTGTCG
ATCGAAATCGCGATCTATCGATGA
ATGCGCTCGCCCCAACGCGGCGGCGCGGCACTGGCGCTGGCCGTCGCCTCGCTCGCCGGATGCGGGGGCGGCGATTCGGG
CGAACCCGCTCCACGCGAATTCGCGCCCCCGACAGTGCAACTCGCCTACCCGACGCAACCGAACTCGCCCGTCGCACCAG
CGCCCACCGCGCACGTATCGAGCGGACACACGCCTCCGGCCACGGCGCCCGCCGCGATGCCCACCGCTTCACCCACCGCG
ACGCCTACCGCTTCACCCACCGCGACGGCTGCCGCTTCGCCCTCCGCGCCATCCACTGCGACGCCCAACGCCCCGCCCGC
CGCTCCGCCGGCCGTCGTCGCCACCCGCGTCCCGCCGACGCACGCGGCGTTGCGCCGCCCGACGATCGAACTCGAATTCG
ATCGCGCGATCGAGCCGGGTTCAGTCCCACACATCGTGCTGCGCGCCGACGATGGCACGAGCGTCGCCGTCGGCCCGTTG
TCGTGGCTGAGCGATCGCCGGATCGCGTTCGCGCCGCGCAAACCGCTCAAGTCGAACAGCCGCTACGAAATCATGGTGCC
CGCCGGCATCAGGAGCACCACGGGCGAACGGTCGGCCCATCCGCTAACGAGCAGCTTCGATACCGCGCCCGTTACGCCGC
CGCGCGGCCTGCCCAATCTCGACGGCGCCTCGTGCTTCATCAACACGGCGCTGCAATTGGCGGTTCACTCGTCGGCGCTC
GACGACATTCTGTCGAACGAAGCCGTCCCGCCCGCCGTCCGCACGCTGCTCGAAGACTACGACGCCGCATCGGCTGATGC
ACTCGACGCGCAGTTGGCCGCCGCGGTCGCCGCGCTGCGCGCCACGCCGGAGGTCCCGGACAGCGGGCCGGGACAAACGC
TGGAAGTGATGCAAGCGTTGCGGATGCCGTTATATGACACGAGCAGCGCGAACAACGCAACGAACAACGCCGACGCCATA
CGTCATGCGCCGCCCAACACCAAGGCGTTCTTTCTGAACTCCTATCCACCGCTTTCCTACGCGGATCTGCCGAACCACGA
CCGGCTCGTCGCGTTCGACTACAGCACGGGCGGTCACTATGTCGCTTATGTGAAGCGGGATGGAATCTGGTATCGAATCG
ACGATGCCCAGGTCAGCGCCGTCAACGAACAGGACTTGCTTGCCCTGCCGGCGTTCAACCCCGCTAACGGCAGCGTGTCG
ATCGAAATCGCGATCTATCGATGA