Detailed information of component protein


Summary

Component ID T6CP000597
Component protein type TssK
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (466 a.a.); Nucleotide sequence (1398 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19550
Coordinate (Strand) 743397..744794 (-)
NCBI ID WP_004206515.1
RefSeq NC_006349
Uniprot ID A5PM27_BURML, A0A0H2XD70_BURMA, A0A6C6YHE1_BURM7, C4ANU4_BURML, A2RXX4_BURM9
KEGG ID bmaf:DM51_4431, bmaz:BM44_4702, bmab:BM45_3156, bmai:DM57_11935, bmal:DM55_4711, bmaq:DM76_3663, bml:BMA10229_0728, bmn:BMA10247_A1686, bmae:DM78_3348, bma:BMAA0732
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 4.5e-105 16..461
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-465 MDNVYWHQGMLLQPQHFQLAELHQQFRFEPWLACGPPHFWGVGALSLAQAAIDRRVVEIRSARLLFADRSYVEYPGNAVVAARAFDPAWLDEGRALVAHVALRRLARGANNVTVAAAPDALPDAPTRYATLPSAEEVADLHSDHPGAPVRTLKHVLKIVFEHELDALAAHETIPIARIVRDGERLRLDDDFAPPCYALSGSRTLLERVRCIRDELAGRARQLQQYKNPREMQRAEFDASYAAFLLALRSLNRFGPLLFHLAECDGQHPWTVYGVLRQLVGELSVFSECFDMLGETPDARGGLPPYDHRDLGGCFSRAHALIGHLLDEIAVGPDCVATFEPDGPQQPAQRSAQLPPDVFADRHQIYLAIRSAHDPDTLAQRFALGGRIAATDEMPQLTALALPGVELTRLPGPPPRLPRRGDARYFRIEQAGRPWDAIRRDGRVSLRWADAPDDLHAELVAVRHTQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9904 - -


  Protein sequence: 466 a.a.    

>T6CP000597 NC_006349:c744794-743397 [Burkholderia mallei ATCC 23344] [TssK]
MDNVYWHQGMLLQPQHFQLAELHQQFRFEPWLACGPPHFWGVGALSLAQAAIDRRVVEIRSARLLFADRSYVEYPGNAVV
AARAFDPAWLDEGRALVAHVALRRLARGANNVTVAAAPDALPDAPTRYATLPSAEEVADLHSDHPGAPVRTLKHVLKIVF
EHELDALAAHETIPIARIVRDGERLRLDDDFAPPCYALSGSRTLLERVRCIRDELAGRARQLQQYKNPREMQRAEFDASY
AAFLLALRSLNRFGPLLFHLAECDGQHPWTVYGVLRQLVGELSVFSECFDMLGETPDARGGLPPYDHRDLGGCFSRAHAL
IGHLLDEIAVGPDCVATFEPDGPQQPAQRSAQLPPDVFADRHQIYLAIRSAHDPDTLAQRFALGGRIAATDEMPQLTALA
LPGVELTRLPGPPPRLPRRGDARYFRIEQAGRPWDAIRRDGRVSLRWADAPDDLHAELVAVRHTQ*

  Nucleotide sequence: 1398 bp    

>T6CP000597 NC_006349:c744794-743397 [Burkholderia mallei ATCC 23344] [TssK]
ATGGACAACGTCTATTGGCATCAGGGGATGCTGCTGCAACCGCAACATTTTCAGTTGGCCGAACTGCACCAGCAGTTCCG
CTTCGAGCCGTGGCTCGCGTGCGGCCCGCCGCATTTCTGGGGCGTCGGCGCGCTGTCGCTCGCGCAGGCCGCGATCGATC
GCCGCGTGGTCGAGATCCGCTCGGCGCGCCTGCTGTTCGCCGACCGCAGCTACGTCGAATATCCGGGCAACGCGGTCGTC
GCCGCGCGCGCGTTCGATCCCGCGTGGCTCGACGAAGGCCGCGCGCTCGTCGCGCACGTCGCGCTCAGGCGGCTCGCGCG
CGGCGCGAACAACGTGACGGTCGCGGCCGCGCCCGACGCGTTGCCCGACGCCCCGACGCGCTACGCGACGCTGCCGTCCG
CCGAGGAGGTCGCCGATCTGCATTCGGACCATCCGGGCGCGCCGGTGCGCACGCTCAAGCACGTGCTGAAGATCGTGTTC
GAGCACGAGCTCGACGCGCTCGCCGCGCACGAAACGATCCCGATCGCGCGGATCGTGCGCGACGGCGAGCGCCTGCGGCT
CGACGACGATTTCGCGCCGCCCTGCTACGCGCTGTCGGGCTCGCGCACGCTGCTCGAGCGCGTGCGCTGCATTCGCGACG
AGCTCGCGGGCCGCGCGCGGCAGTTGCAGCAGTACAAGAATCCGCGCGAGATGCAACGCGCCGAATTCGACGCGAGCTAT
GCGGCGTTCCTGCTCGCGCTGCGCTCGCTGAACCGCTTCGGCCCGCTGCTGTTCCATCTCGCCGAATGCGACGGGCAGCA
TCCATGGACGGTCTACGGCGTGCTGCGCCAACTCGTCGGCGAGCTGAGCGTGTTCTCCGAGTGCTTCGACATGCTCGGCG
AGACGCCCGATGCGCGCGGCGGCCTGCCGCCGTACGACCACCGCGATCTGGGCGGCTGCTTCTCGCGCGCGCACGCGCTG
ATCGGCCACCTGCTCGACGAAATCGCGGTGGGCCCGGACTGCGTCGCGACGTTCGAGCCCGACGGCCCGCAGCAGCCCGC
GCAACGCTCGGCGCAACTGCCGCCCGACGTGTTCGCGGATCGCCACCAGATCTATCTCGCGATCCGCAGCGCGCACGATC
CGGACACGCTCGCGCAACGCTTCGCGCTCGGCGGCCGGATCGCGGCGACCGACGAAATGCCGCAGCTCACCGCGCTCGCG
CTGCCGGGCGTCGAACTCACCCGCCTGCCCGGCCCGCCGCCGCGGCTGCCGCGCCGCGGCGACGCGCGCTACTTCCGGAT
CGAGCAGGCCGGCCGCCCGTGGGACGCGATCCGGCGTGACGGCCGCGTGTCGCTGCGCTGGGCCGACGCGCCCGACGACC
TGCACGCGGAACTCGTCGCAGTGAGGCACACGCAATGA