Detailed information of component protein


Summary

Component ID T6CP008460
Component protein type TssE
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (154 a.a.); Nucleotide sequence (462 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19600
Coordinate (Strand) 759472..759933 (-)
NCBI ID WP_004184744.1
RefSeq NC_006349
Uniprot ID A5PM45_BURML, A0A0H2XDM9_BURMA, C4B101_BURML, A0A7U8D3G9_BURML, A2RXW4_BURM9
KEGG ID bma:BMAA0741, bmaf:DM51_4441, bmaq:DM76_3653, bmal:DM55_4701, bmab:BM45_3166, bmae:DM78_3358, bml:BMA10229_0718, bmai:DM57_11885
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
GPW_gp25 PF04965.16 3.1e-16 41..128
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-153 MADRESSRAGEPRTLERRLLERIANREAGGEHDRPPPADVLAHSIIDHLRRILNTRQGHVPIDPAFGVPDFTNLAGGFAQGSAREIEAQIERVITCYEPRLKAPRVSLAERGPDAATLRFSLDARLVIDAHDVPARFLTTVTGNGKIDIRTIS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9814 - -


  Protein sequence: 154 a.a.    

>T6CP008460 NC_006349:c759933-759472 [Burkholderia mallei ATCC 23344] [TssE]
MADRESSRAGEPRTLERRLLERIANREAGGEHDRPPPADVLAHSIIDHLRRILNTRQGHVPIDPAFGVPDFTNLAGGFAQ
GSAREIEAQIERVITCYEPRLKAPRVSLAERGPDAATLRFSLDARLVIDAHDVPARFLTTVTGNGKIDIRTIS*

  Nucleotide sequence: 462 bp    

>T6CP008460 NC_006349:c759933-759472 [Burkholderia mallei ATCC 23344] [TssE]
ATGGCTGATCGCGAATCGTCGCGGGCCGGCGAGCCGCGCACGCTCGAGCGCCGGCTGCTCGAGCGGATCGCGAACCGGGA
AGCGGGCGGCGAGCACGATCGCCCGCCGCCCGCCGACGTGCTCGCGCACTCGATCATCGACCACCTGCGCCGCATCCTGA
ACACGCGGCAGGGCCACGTGCCGATCGATCCCGCCTTCGGCGTGCCGGATTTCACGAATCTCGCGGGCGGCTTCGCGCAA
GGCTCCGCCCGCGAAATCGAAGCGCAGATCGAGCGCGTGATCACTTGCTACGAGCCGCGGCTGAAAGCGCCGCGCGTCTC
GCTCGCCGAGCGCGGGCCCGATGCGGCGACGCTGCGCTTCAGCCTCGATGCGCGGCTCGTGATCGACGCGCACGACGTGC
CGGCGCGCTTTCTGACGACCGTCACCGGCAACGGAAAGATCGATATCCGAACGATTTCCTGA