Detailed information of component protein


Summary

Component ID T6CP008459
Component protein type TssM
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (1349 a.a.); Nucleotide sequence (4047 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19540
Coordinate (Strand) 738692..742738 (-)
NCBI ID WP_174977041.1
RefSeq NC_006349
Uniprot ID UPI0000558F42
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.4e-55 177..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKTLGM
Transmembrane helix 7-26 MIARIAALLALAALSWASAL
Outside 27-29 YFD
Transmembrane helix 30-52 WPLWCAPAVFCAALAGWLLCRVA
Inside 53-414 RRALRAVRARAQLARLDASERLPAADAPELRVAARWRTALAALGRAQPAPRLRGRPRDALPWYLVIGPEGAGKTTALARARLASPLRHARDDAAIAPTEDCDWWCFDDAVVLDLAGRFAAPGATDDDRRAWHALLEQLGRARARRGVNGVVVAIDAPRLIEASRDALTIEGSAIRERLEQLIRLFDRRFPVYVLVTQCDRLYGFDEWAAQLAPEQRERAFGYLGDHDADAFVARALDSLDARLGALRVALAARGEPPSPRALMLPHELACLRPALEAFARAAFGPNVYQETPYLRGLLFASGRQAGGAPSLTLPDWLDAAPARTPGDAGLFLHDVFARVLPGERDAWLPVERPSRSRLVLRR
Transmembrane helix 415-437 LALAAWLLASVAAGLLMSASFVG
Outside 438-1348 DMRTVELVRRDYPAHPRFTGEFAHDTATLARIGRVIADVERRDERRLVRPLAGATPVGRLEAELKRRYVAHYRRSIEPTADRLFFGKPDGASDMRAANDARLAARIRNLVRYVNLMQARRRGADRETLAQMPAPAVARATDGGGARGVDSVGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGGVGGVGGVGGARAAEGAGRAVRASGGRRAGDARAARGMRGAAGNAGAGAEIAPAADGVRDRDEGFARVSALAVDLIAWSAPDDRALAARVAAAQAELERLAYRDPDGAWLLALPDASMPRDVTLADFWPAAEPRTLPDAQPAALRDVRVPAALTAAHRPAIDAFLDEMAQAVANRPKFAFHRDAFDTWYRARRIDAWRDFVARFPQGEQGLTTQAQWRAVIDRIADHRDPFAALLARVDREFESERDDTLPPWLRFVRTTSHMLAPAMLAPARLPAASGGLGAALGSISRSGGRALREALGGAPEQGRLTLERDAALRDALVDYERRVAALAADALAGPGAAYRLAADFHGFGVDPSVQASAMRAADSALRDVKRLAGDRDVGGDVVWSLVGGPLRALIAYVERQASCALQDDWERDVLWPLRRAATRDDVEGLLYGPQGAIWAFVDGPAKPFVRVGAARASALDTLGYRLPFTDAFLPLIDDAAARRVAQARRDAERRAQQQAALELDKRIASLGKQIDALRAQTVRFGIVAQPTDVNPDAQAKPFETVLTLQCAPQARTLTNYNLRVSEQIDWQPDRCGDATLRISLGGVTLVRRYAGSLGVARFVQDFRYGVRRFTPRDFPDAKAQLQRLGVRHIDVRYDFSGHDALLAHVGRIDALERARRDDLARQRRAANRQDDDAGGATAIARAAASVANAPGAALPRRIGVCWGDPAHDRPDGDGAQP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8003 - -


  Protein sequence: 1349 a.a.    

>T6CP008459 NC_006349:c742738-738692 [Burkholderia mallei ATCC 23344] [TssM]
MKTLGMMIARIAALLALAALSWASALYFDWPLWCAPAVFCAALAGWLLCRVARRALRAVRARAQLARLDASERLPAADAP
ELRVAARWRTALAALGRAQPAPRLRGRPRDALPWYLVIGPEGAGKTTALARARLASPLRHARDDAAIAPTEDCDWWCFDD
AVVLDLAGRFAAPGATDDDRRAWHALLEQLGRARARRGVNGVVVAIDAPRLIEASRDALTIEGSAIRERLEQLIRLFDRR
FPVYVLVTQCDRLYGFDEWAAQLAPEQRERAFGYLGDHDADAFVARALDSLDARLGALRVALAARGEPPSPRALMLPHEL
ACLRPALEAFARAAFGPNVYQETPYLRGLLFASGRQAGGAPSLTLPDWLDAAPARTPGDAGLFLHDVFARVLPGERDAWL
PVERPSRSRLVLRRLALAAWLLASVAAGLLMSASFVGDMRTVELVRRDYPAHPRFTGEFAHDTATLARIGRVIADVERRD
ERRLVRPLAGATPVGRLEAELKRRYVAHYRRSIEPTADRLFFGKPDGASDMRAANDARLAARIRNLVRYVNLMQARRRGA
DRETLAQMPAPAVARATDGGGARGVDSVGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGGVGGVGGVGGARAAEGAG
RAVRASGGRRAGDARAARGMRGAAGNAGAGAEIAPAADGVRDRDEGFARVSALAVDLIAWSAPDDRALAARVAAAQAELE
RLAYRDPDGAWLLALPDASMPRDVTLADFWPAAEPRTLPDAQPAALRDVRVPAALTAAHRPAIDAFLDEMAQAVANRPKF
AFHRDAFDTWYRARRIDAWRDFVARFPQGEQGLTTQAQWRAVIDRIADHRDPFAALLARVDREFESERDDTLPPWLRFVR
TTSHMLAPAMLAPARLPAASGGLGAALGSISRSGGRALREALGGAPEQGRLTLERDAALRDALVDYERRVAALAADALAG
PGAAYRLAADFHGFGVDPSVQASAMRAADSALRDVKRLAGDRDVGGDVVWSLVGGPLRALIAYVERQASCALQDDWERDV
LWPLRRAATRDDVEGLLYGPQGAIWAFVDGPAKPFVRVGAARASALDTLGYRLPFTDAFLPLIDDAAARRVAQARRDAER
RAQQQAALELDKRIASLGKQIDALRAQTVRFGIVAQPTDVNPDAQAKPFETVLTLQCAPQARTLTNYNLRVSEQIDWQPD
RCGDATLRISLGGVTLVRRYAGSLGVARFVQDFRYGVRRFTPRDFPDAKAQLQRLGVRHIDVRYDFSGHDALLAHVGRID
ALERARRDDLARQRRAANRQDDDAGGATAIARAAASVANAPGAALPRRIGVCWGDPAHDRPDGDGAQP*

  Nucleotide sequence: 4047 bp    

>T6CP008459 NC_006349:c742738-738692 [Burkholderia mallei ATCC 23344] [TssM]
ATGAAGACTCTCGGCATGATGATCGCGCGGATCGCCGCGCTGCTCGCGCTCGCCGCGCTCAGTTGGGCGAGCGCGCTCTA
TTTCGACTGGCCGCTCTGGTGCGCGCCCGCCGTGTTCTGCGCGGCGCTCGCCGGATGGCTGCTGTGCCGCGTCGCGCGCC
GCGCGCTGCGCGCGGTTCGTGCGCGCGCGCAGCTCGCACGGCTCGACGCATCCGAGCGCCTGCCCGCGGCCGACGCGCCG
GAGCTGCGCGTCGCCGCGCGCTGGCGCACCGCGCTCGCCGCGCTCGGGCGCGCGCAGCCCGCGCCGCGCCTGCGCGGCCG
GCCGCGCGACGCGCTGCCGTGGTATCTCGTGATCGGCCCCGAGGGCGCGGGCAAGACAACCGCGCTCGCGCGCGCGCGCC
TCGCGTCGCCGCTGCGGCACGCGCGCGACGACGCGGCCATCGCGCCGACCGAGGACTGCGACTGGTGGTGCTTCGACGAC
GCGGTCGTGCTCGATCTCGCGGGCCGCTTCGCCGCGCCCGGCGCGACCGACGACGATCGCCGCGCATGGCACGCGCTCCT
CGAGCAGCTCGGCCGCGCGCGTGCGCGCCGGGGCGTGAACGGCGTCGTGGTCGCGATCGACGCGCCGCGCCTCATCGAAG
CGAGCCGCGATGCGCTGACGATCGAAGGCAGCGCCATTCGCGAACGGCTCGAGCAACTGATCCGCCTGTTCGACCGGCGC
TTTCCGGTGTACGTGCTCGTCACGCAATGCGACCGGCTCTACGGCTTCGACGAATGGGCCGCGCAGCTCGCGCCGGAACA
ACGCGAGCGCGCGTTCGGCTATCTCGGCGATCACGACGCGGATGCGTTCGTCGCGCGCGCGCTCGACAGCCTCGACGCAC
GGCTCGGCGCGCTGCGCGTCGCGCTGGCGGCGCGCGGCGAGCCGCCGTCGCCGCGCGCGCTGATGCTGCCGCACGAACTC
GCGTGCCTGCGGCCCGCGCTCGAGGCGTTCGCACGCGCGGCGTTCGGCCCGAACGTCTATCAGGAAACACCGTATCTGCG
CGGGCTGCTGTTCGCGAGCGGCCGGCAGGCGGGCGGCGCGCCGTCGCTGACGCTGCCCGACTGGCTCGACGCCGCGCCGG
CGCGCACGCCCGGCGATGCGGGCCTGTTCCTGCACGACGTGTTCGCGCGCGTGCTGCCGGGCGAGCGCGACGCGTGGCTG
CCCGTCGAGCGGCCGAGCCGCTCGCGGCTCGTGCTGCGGCGCCTCGCGCTCGCCGCGTGGCTCCTCGCGAGCGTCGCGGC
CGGGCTGCTGATGAGCGCATCGTTCGTCGGCGACATGCGAACCGTCGAGCTGGTCCGCCGCGACTATCCCGCGCATCCGC
GCTTCACCGGCGAATTCGCGCACGACACGGCGACGCTCGCGCGGATCGGCCGCGTGATCGCCGACGTCGAGCGGCGCGAC
GAGCGGCGGCTCGTGCGCCCGCTCGCCGGCGCGACGCCCGTCGGCCGGCTCGAAGCGGAACTCAAGCGCCGCTACGTCGC
ACACTATCGGCGCTCGATCGAACCCACCGCCGATCGGCTGTTCTTCGGCAAGCCGGACGGCGCGAGCGACATGCGCGCGG
CCAACGATGCGCGGCTCGCCGCGCGCATCCGCAATCTGGTCCGCTACGTGAACCTGATGCAGGCGCGCCGGCGCGGCGCG
GATCGCGAGACGCTCGCGCAAATGCCCGCACCGGCGGTCGCGCGCGCGACGGATGGCGGTGGCGCTCGCGGAGTGGACAG
TGTGGGTGTTTCTGGTGTTTCTGGTGTTTCTGGTGTTTCTGGTGTTTCTGGTGTTTCTGGTGTTTCTGGTGTTTCTGGTG
TTTCTGGTGTTTCTGGTGTTTCTGGCGGTGTAGGCGGTGTAGGCGGTGTAGGCGGTGCGCGCGCCGCGGAAGGTGCGGGT
CGCGCCGTCCGCGCGAGCGGCGGACGGCGCGCAGGCGACGCGCGCGCCGCGCGCGGCATGCGCGGCGCCGCCGGCAACGC
CGGCGCCGGCGCTGAAATCGCACCCGCCGCAGACGGCGTTCGCGATCGCGACGAAGGGTTTGCGCGCGTCAGCGCGCTCG
CCGTCGACCTGATCGCGTGGAGCGCGCCCGACGATCGCGCGCTCGCCGCGCGCGTCGCGGCCGCGCAGGCAGAGCTCGAA
CGGCTCGCGTACCGCGATCCGGACGGCGCGTGGCTGCTCGCGCTACCGGATGCCTCGATGCCGCGCGACGTGACGCTCGC
CGATTTCTGGCCCGCCGCCGAGCCGCGCACGCTGCCCGACGCACAGCCCGCCGCGCTGCGCGACGTGCGCGTGCCCGCCG
CGCTGACGGCCGCGCATCGCCCGGCCATCGATGCGTTCCTCGACGAGATGGCGCAGGCGGTCGCGAACCGGCCGAAGTTC
GCGTTCCATCGCGACGCGTTCGACACGTGGTACCGCGCACGGCGCATCGACGCATGGCGCGACTTCGTCGCACGCTTCCC
GCAGGGCGAGCAAGGGCTGACGACGCAAGCGCAATGGCGCGCGGTGATCGACCGGATCGCCGATCACCGCGATCCGTTCG
CGGCATTGCTCGCGCGCGTCGATCGCGAATTCGAATCCGAGCGCGACGACACGCTGCCGCCGTGGCTACGGTTCGTGCGC
ACGACATCGCACATGCTCGCCCCCGCGATGCTCGCGCCCGCGCGCCTGCCCGCGGCGAGCGGCGGCCTGGGCGCCGCGCT
CGGCTCGATCTCGCGCAGCGGCGGACGCGCGCTGCGCGAAGCGCTCGGCGGCGCACCCGAGCAAGGCAGGCTCACGCTCG
AGCGCGACGCCGCGCTGCGCGACGCGCTTGTCGACTACGAGCGGCGCGTCGCCGCGCTCGCCGCCGACGCGCTCGCGGGC
CCCGGCGCCGCGTACCGGCTCGCGGCCGATTTCCACGGCTTCGGCGTCGATCCGTCCGTCCAGGCTTCCGCGATGCGCGC
GGCCGACAGCGCGCTGCGCGACGTCAAGCGGCTCGCGGGCGATCGCGACGTCGGCGGCGACGTCGTCTGGAGCCTCGTCG
GCGGCCCGCTGCGCGCGCTGATCGCGTATGTCGAGCGGCAGGCGTCGTGCGCGCTGCAGGACGACTGGGAGCGCGACGTG
CTGTGGCCGCTCAGGCGCGCGGCGACGCGCGACGACGTGGAGGGTCTGCTGTACGGCCCGCAAGGCGCGATCTGGGCGTT
CGTCGACGGCCCGGCGAAGCCGTTCGTGCGTGTCGGCGCGGCGCGTGCGTCGGCGCTCGACACGCTCGGCTACCGGCTGC
CGTTCACCGACGCGTTCCTGCCGCTCATCGACGACGCGGCCGCCCGGCGCGTCGCGCAAGCGCGGCGCGACGCCGAGCGG
CGCGCGCAACAGCAGGCGGCGCTCGAGCTGGACAAGCGGATCGCATCGCTCGGCAAGCAGATCGACGCGCTGCGCGCGCA
AACGGTGCGCTTCGGGATCGTCGCGCAGCCGACCGACGTGAACCCGGATGCGCAGGCCAAGCCGTTCGAAACCGTGCTGA
CGCTGCAATGCGCGCCGCAGGCGCGCACGCTGACGAACTACAACCTGCGCGTGTCCGAGCAGATCGACTGGCAACCCGAT
CGATGCGGCGACGCGACGCTGCGCATCTCGCTCGGCGGCGTCACGCTCGTGCGCCGTTACGCGGGATCGCTCGGCGTCGC
GCGCTTCGTCCAGGACTTCCGCTACGGCGTGCGCCGCTTCACGCCCCGCGATTTCCCGGACGCGAAAGCGCAGCTTCAAC
GTCTCGGCGTGCGCCACATCGACGTGCGTTACGACTTCTCCGGGCATGACGCGCTGCTCGCGCACGTCGGTCGGATCGAC
GCGCTCGAACGCGCGCGCCGCGACGACCTCGCGCGGCAGCGGCGCGCCGCGAACCGGCAGGACGACGACGCGGGCGGCGC
GACGGCGATCGCGCGGGCGGCGGCGTCCGTCGCGAATGCGCCGGGCGCCGCGCTGCCCCGCCGCATCGGCGTGTGTTGGG
GGGACCCGGCGCACGACAGGCCCGATGGCGACGGCGCGCAACCGTGA