Detailed information of component protein


Summary

Component ID T6CP000600
Component protein type TssG
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (341 a.a.); Nucleotide sequence (1023 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19590
Coordinate (Strand) 756683..757705 (-)
NCBI ID WP_004200970.1
RefSeq NC_006349
Uniprot ID A5PM47_BURML, A0A0H2WE15_BURMA, A0A6C6YKY2_BURM7, C4B0Z9_BURML, A2RXW6_BURM9
KEGG ID bmaf:DM51_4439, bmaz:BM44_4694, bmaq:DM76_3655, bmn:BMA10247_A1678, bmal:DM55_4703, bml:BMA10229_0720, bma:BMAA0739, bmab:BM45_3164, bmai:DM57_11895
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssG PF06996.13 2e-85 12..311
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-340 MPALEPVLLGEAKHFAYFQAIRLLRRIARERRGDAAGRPDAPLPIHTRPNLSLSFPDTDVERIDKADDGGYRVVANFFGLYGVSSPLPTFYTEDLIDEAFRGRHAARGFLDVLHRALYPLLFDAWLKHRLALRIVEERDEHALRPLYALAGVDARIARDAGLHEHALLRYVGLLSQRPRSAAGLRALLADAFAPATVDIEPCVSQWLPIPDDQRTRVGTRARRLGADARVGARMRDDGARLRIVLGDVPGPLFRALMPGGDAFARLRLLVRLYLTQPFAVDVVVRVRARDAAPARCGRRAWSRVGLDAWLGGPSAERAASPKFRLPTSLFDQARPHHAAG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9884 - -


  Protein sequence: 341 a.a.    

>T6CP000600 NC_006349:c757705-756683 [Burkholderia mallei ATCC 23344] [TssG]
MPALEPVLLGEAKHFAYFQAIRLLRRIARERRGDAAGRPDAPLPIHTRPNLSLSFPDTDVERIDKADDGGYRVVANFFGL
YGVSSPLPTFYTEDLIDEAFRGRHAARGFLDVLHRALYPLLFDAWLKHRLALRIVEERDEHALRPLYALAGVDARIARDA
GLHEHALLRYVGLLSQRPRSAAGLRALLADAFAPATVDIEPCVSQWLPIPDDQRTRVGTRARRLGADARVGARMRDDGAR
LRIVLGDVPGPLFRALMPGGDAFARLRLLVRLYLTQPFAVDVVVRVRARDAAPARCGRRAWSRVGLDAWLGGPSAERAAS
PKFRLPTSLFDQARPHHAAG*

  Nucleotide sequence: 1023 bp    

>T6CP000600 NC_006349:c757705-756683 [Burkholderia mallei ATCC 23344] [TssG]
ATGCCTGCTCTAGAACCCGTTCTGCTCGGCGAGGCGAAGCACTTCGCGTATTTCCAGGCGATCCGGCTGCTGCGGCGCAT
CGCGCGCGAACGGCGCGGCGACGCCGCGGGGCGGCCCGACGCGCCGCTGCCGATCCACACGCGGCCGAATCTCTCGCTGT
CGTTTCCGGACACCGACGTCGAGCGCATCGACAAGGCCGACGACGGCGGCTACCGGGTCGTCGCGAACTTCTTCGGTCTG
TACGGCGTGTCCTCGCCGCTGCCGACGTTCTACACCGAGGACCTGATCGACGAAGCGTTCAGGGGCCGCCACGCGGCGCG
CGGCTTTCTCGACGTGCTGCATCGCGCGCTCTATCCGCTGCTGTTCGACGCGTGGCTCAAGCATCGGCTGGCGCTGCGGA
TCGTCGAGGAACGCGACGAACACGCGCTGCGCCCGCTCTACGCACTCGCGGGCGTCGACGCGCGCATCGCGCGCGACGCG
GGCCTGCACGAGCACGCGCTGCTGCGCTACGTCGGGCTGCTCAGCCAGCGGCCGCGCTCCGCGGCCGGTCTGCGCGCGCT
GCTCGCCGACGCGTTCGCGCCCGCGACGGTCGACATCGAGCCGTGCGTGTCGCAATGGCTGCCGATTCCGGACGACCAGC
GCACCCGCGTCGGCACGCGCGCGCGCCGGCTCGGCGCCGACGCGCGGGTCGGCGCGCGCATGCGCGACGACGGCGCCCGG
CTGCGGATCGTGCTCGGCGACGTGCCCGGCCCGCTGTTCCGCGCGCTGATGCCGGGCGGCGATGCGTTTGCCCGGCTGCG
CTTGCTCGTACGCCTGTATCTGACCCAACCGTTCGCCGTGGACGTCGTGGTGCGCGTGCGCGCGCGCGACGCGGCCCCCG
CGCGCTGCGGCCGCCGCGCGTGGTCGCGCGTCGGGCTCGACGCGTGGCTGGGCGGCCCGAGCGCGGAACGCGCGGCGTCG
CCCAAATTCCGTCTTCCGACTTCCCTCTTTGATCAAGCGAGACCCCATCATGCTGCTGGTTGA