Detailed information of component protein


Summary

Component ID T6CP000596
Component protein type TssL
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (222 a.a.); Nucleotide sequence (666 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19545
Coordinate (Strand) 742735..743400 (-)
NCBI ID WP_004184888.1
RefSeq NC_006349
Uniprot ID A5PM28_BURML, A0A0F6L1K5_BURPE, A0A0H2XDM1_BURMA, A0A095JNC2_BURPE, A0A0E1VRX1_BURPE, Q3JL48_BURP1, B1H8J6_BURPE, A0A6C6YKD9_BURM7, C4ANU3_BURML, A0A7U8D3H0_BURML, A3P6W2_BURP0, A8EPN8_BURPE, A2RXX5_BURM9, A0A0E1S5J4_BURPE, C4I6H2_BURPE, A0A0E1TPF1_BURPE, A0A0E1ULN3_BURPE, A0A0H3HU22_BURP2
KEGG ID bpl:BURPS1106A_A2039, bmae:DM78_3347, bmaf:DM51_4430, bmab:BM45_3155, bpz:BP1026B_II1603, bml:BMA10229_0729, but:X994_5605, bmaz:BM44_4703, bmaq:DM76_3664, bma:BMAA0731, bmal:DM55_4712, bmai:DM57_11940, bmn:BMA10247_A1687, bpm:BURPS1710b_A0546
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3.3e-43 3..207
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-186 MKLTDSMMPLVAYARQLAQSPRGDAAEVALRLDILIERARDHAACAGAPAADVDDALFAVCAWIDEALLNSGWDDADRWTLRLLQKRYFDTSHAGVVFFERLGTLDGARASVLEVYLLCLRLGFRGRYGYDGGDGPLDAIRQRALDALSAHAPPCAAGEPPFPAAYAHAGPHAAAAKARTRRRIAR
Transmembrane helix 187-209 AGLSVGLPLAILLSLYLIYHVVI
Outside 210-221 VRMVDAVYPRLQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9492 - -


  Protein sequence: 222 a.a.    

>T6CP000596 NC_006349:c743400-742735 [Burkholderia mallei ATCC 23344] [TssL]
MKLTDSMMPLVAYARQLAQSPRGDAAEVALRLDILIERARDHAACAGAPAADVDDALFAVCAWIDEALLNSGWDDADRWT
LRLLQKRYFDTSHAGVVFFERLGTLDGARASVLEVYLLCLRLGFRGRYGYDGGDGPLDAIRQRALDALSAHAPPCAAGEP
PFPAAYAHAGPHAAAAKARTRRRIARAGLSVGLPLAILLSLYLIYHVVIVRMVDAVYPRLQ*

  Nucleotide sequence: 666 bp    

>T6CP000596 NC_006349:c743400-742735 [Burkholderia mallei ATCC 23344] [TssL]
ATGAAGCTGACCGATTCGATGATGCCGCTCGTCGCGTATGCGCGGCAGCTCGCGCAATCGCCGCGCGGCGACGCCGCCGA
AGTCGCGCTGCGGCTCGACATCCTGATCGAGCGCGCGCGCGACCACGCGGCGTGCGCGGGCGCACCCGCGGCCGACGTCG
ACGACGCGCTGTTCGCGGTGTGCGCGTGGATCGACGAGGCGCTGCTGAACAGCGGCTGGGACGATGCCGATCGCTGGACG
CTGCGGCTGCTGCAAAAGCGCTACTTCGATACGTCGCACGCGGGCGTCGTGTTCTTCGAACGCCTCGGCACGCTCGACGG
CGCGCGCGCGAGCGTGCTCGAGGTCTACCTGCTGTGCCTGCGGCTCGGCTTTCGCGGCCGCTACGGCTACGACGGCGGCG
ACGGCCCGCTCGACGCGATCCGCCAGCGCGCGCTCGATGCACTGTCGGCGCACGCGCCGCCGTGCGCCGCGGGCGAGCCG
CCGTTTCCCGCCGCCTATGCGCACGCCGGCCCGCACGCCGCCGCCGCGAAAGCGCGCACGCGCCGCCGGATCGCGCGCGC
GGGGCTGAGCGTCGGGCTGCCGCTCGCCATCCTGCTGTCGCTCTATCTGATCTATCACGTCGTCATCGTGCGGATGGTCG
ACGCGGTGTACCCCAGGCTGCAATGA