Detailed information of regulator


Summary

Regulator ID REG0129 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-RR
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome
Sequence Protein sequence (246 a.a.); Nucleotide sequence (738 bp)
Description Acid pH upregulates TssM and Hcp1 expression through VirAG.
Reference
[1] Chua J, Senft JL, Lockett SJ, et al. pH Alkalinization by Chloroquine Suppresses Pathogenic Burkholderia Type 6 Secretion System 1 and Multinucleated Giant Cells. Infect Immun. 2016 Dec 29;85(1). pii: e00586-16. doi: 10.1128/IAI.00586-16. Print 2017 Jan. PMID: 27799332
[2] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[3] Burtnick MN, Brett PJ. Burkholderia mallei and Burkholderia pseudomallei cluster 1 type VI secretion system gene expression is negatively regulated by iron and zinc. PLoS One. 2013 Oct 11;8(10):e76767. doi: 10.1371/journal.pone.0076767. eCollection 2013. PMID: 24146925
[4] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
External database links

Locus tag (Gene) BMAA0746 (VirG)
Coordinates (Strand) 764773..765510 (+)
NCBI ID 53717366
RefSeq NC_006349
Uniprot ID A0A0H2WCL9_BURMA
KEGG ID bma:BMAA0745
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
BMAA0742 (Hcp1) expression ↓ 77358963
BMAA0729.1 (TssM1) expression ↓ 77358930
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Trans_reg_C PF00486.30 4.4e-20 161..235
Response_reg PF00072.26 7.4e-20 10..118
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-245 MTNVTRSPAVLVVEDDEPLRRVLCNYLSGHGMAVDGVGTAAAAAESVGRREYEMVLLDLGLPDGDGIDVLLRWRETQRFPLICVTGRSEEADRIMGLEFGADDYVVKPYSLREVLARMRALWRRAEPSTTPVRRGRHPRAYRFDGWTLNMNTRRLLTRDAQEIPLTVGEFNLLAFFLGSPSRVVTRAQLLECTRAFDDVFDRAVDVQIWRLRKKIEKDPKHPELIRTERGVGYYFDAKEVETLWG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8795 - -


  Protein sequence of regulator: 246 a.a.    .

>REG0129 NC_006349:764773-765510 [Burkholderia mallei ATCC 23344]
MTNVTRSPAVLVVEDDEPLRRVLCNYLSGHGMAVDGVGTAAAAAESVGRREYEMVLLDLGLPDGDGIDVLLRWRETQRFP
LICVTGRSEEADRIMGLEFGADDYVVKPYSLREVLARMRALWRRAEPSTTPVRRGRHPRAYRFDGWTLNMNTRRLLTRDA
QEIPLTVGEFNLLAFFLGSPSRVVTRAQLLECTRAFDDVFDRAVDVQIWRLRKKIEKDPKHPELIRTERGVGYYFDAKEV
ETLWG*

  Nucleotide sequence of regulator: 738 bp    .

>REG0129 NC_006349:764773-765510 [Burkholderia mallei ATCC 23344]
ATGACGAATGTGACAAGATCGCCGGCGGTGCTCGTCGTCGAGGACGACGAACCGTTGCGGCGCGTGCTTTGCAATTACCT
GTCGGGCCACGGGATGGCCGTGGACGGCGTGGGAACGGCGGCAGCGGCCGCCGAATCGGTCGGGCGGCGCGAATACGAGA
TGGTGCTGCTCGATCTCGGCCTGCCGGACGGCGACGGCATCGACGTGCTGCTGCGCTGGCGCGAAACGCAGCGCTTTCCG
CTCATCTGCGTGACGGGGCGCAGCGAAGAGGCCGATCGCATCATGGGCCTCGAATTCGGCGCGGACGATTACGTCGTGAA
GCCGTACTCGCTGCGCGAGGTGCTCGCCCGGATGCGCGCGCTGTGGCGGCGCGCCGAGCCGTCGACGACGCCCGTGCGGC
GCGGCAGGCATCCGCGTGCGTACCGCTTCGACGGATGGACGCTGAACATGAACACGCGCCGGCTGTTGACGCGCGATGCG
CAGGAAATTCCGCTGACGGTCGGCGAATTCAACCTGCTCGCGTTCTTTCTCGGCTCGCCGTCGCGCGTCGTCACGCGCGC
GCAGTTGCTCGAGTGCACGCGTGCGTTCGACGACGTGTTCGATCGCGCGGTCGACGTGCAGATCTGGCGGCTGCGCAAGA
AAATCGAGAAGGATCCGAAGCATCCCGAACTGATTCGAACCGAGCGGGGCGTCGGCTACTACTTCGACGCGAAAGAGGTG
GAGACGCTATGGGGATGA