Detailed information of component protein


Summary

Component ID T6CP012439
Component protein type VirA
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (615 a.a.); Nucleotide sequence (1845 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19620
Coordinate (Strand) 762932..764776 (+)
NCBI ID WP_004200976.1
RefSeq NC_006349
Uniprot ID A5PM42_BURML, A0A6C6YJJ2_BURM7, C4B107_BURML, A0A7U8D6M6_BURML, A0A0E1TMJ9_BURPE
KEGG ID bmaz:BM44_4688, bmn:BMA10247_A1670, bmaf:DM51_4445, bmae:DM78_3362, bmai:DM57_11865
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
HATPase_c PF02518.28 6.9e-18 454..592
MASE1 PF05231.16 2.2e-13 63..323
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-32 MHGNSIDAGPPARARPARITRTARTAPRRDDR
Transmembrane helix 33-52 AFAPGGALNAAGLLLLLFYA
Outside 53-66 NHLLSRPGACTSLN
Transmembrane helix 67-86 HTLSAVVWPAPVLGIALLLH
Inside 87-92 CRRPLA
Transmembrane helix 93-115 WWLGAGSLFATLLAAGSLDWVPW
Outside 116-118 HVD
Transmembrane helix 119-141 ALFAAVNVVEAIAGAWLARRYLI
Inside 142-153 AGGGVDTLRGFA
Transmembrane helix 154-176 MFVLLLPLGMAAMHATLAAAILS
Outside 177-190 VWMNDREWLSEWSR
Transmembrane helix 191-213 AYAANALALLVLLPALLTWNARA
Inside 214-224 CGAAWSRSRNQ
Transmembrane helix 225-247 LPVLATFGSLCFASIWGYQAEVA
Outside 248-261 RALLALSLSWAALE
Transmembrane helix 262-284 GGLVLASQLNALSAIALIAMTLG
Inside 285-296 GRGPYAWHHDGH
Transmembrane helix 297-319 GVWALQVDLIGVAILSMCIAIAT
Outside 320-614 GERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARRGEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVRVGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEAAMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGLAIVAGAALAHRGQIAVATDRGAGSCFRLTLPAAWGTGAPGAMDAGAPGAVQERT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8692 - -


  Protein sequence: 615 a.a.    

>T6CP012439 NC_006349:762932-764776 [Burkholderia mallei ATCC 23344] [VirA]
MHGNSIDAGPPARARPARITRTARTAPRRDDRAFAPGGALNAAGLLLLLFYANHLLSRPGACTSLNHTLSAVVWPAPVLG
IALLLHCRRPLAWWLGAGSLFATLLAAGSLDWVPWHVDALFAAVNVVEAIAGAWLARRYLIAGGGVDTLRGFAMFVLLLP
LGMAAMHATLAAAILSVWMNDREWLSEWSRAYAANALALLVLLPALLTWNARACGAAWSRSRNQLPVLATFGSLCFASIW
GYQAEVARALLALSLSWAALEGGLVLASQLNALSAIALIAMTLGGRGPYAWHHDGHGVWALQVDLIGVAILSMCIAIATG
ERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARR
GEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVR
VGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEAAMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGL
AIVAGAALAHRGQIAVATDRGAGSCFRLTLPAAWGTGAPGAMDAGAPGAVQERT*

  Nucleotide sequence: 1845 bp    

>T6CP012439 NC_006349:762932-764776 [Burkholderia mallei ATCC 23344] [VirA]
ATGCACGGAAATTCCATCGATGCCGGGCCGCCCGCGCGCGCCCGGCCCGCACGCATCACGCGAACGGCGCGCACCGCGCC
GCGCCGCGACGATCGCGCGTTCGCGCCGGGCGGCGCGCTGAACGCGGCGGGGCTGCTGCTGCTGCTGTTCTACGCGAATC
ATCTGCTGTCCCGGCCCGGCGCGTGCACGTCGCTCAATCACACGCTGTCGGCCGTCGTGTGGCCCGCGCCGGTGCTCGGC
ATCGCGCTGCTGCTGCACTGCCGGCGGCCGCTCGCATGGTGGCTCGGCGCGGGTTCGCTGTTCGCCACGCTGCTCGCCGC
GGGCTCGCTCGACTGGGTGCCGTGGCACGTCGACGCGCTGTTCGCGGCCGTCAACGTCGTCGAGGCGATCGCGGGCGCGT
GGCTCGCGCGGCGCTATCTGATCGCGGGCGGCGGGGTGGACACGCTGCGCGGCTTCGCGATGTTCGTGCTGCTGCTGCCG
CTCGGCATGGCGGCGATGCACGCGACGCTCGCGGCGGCGATCCTGTCGGTATGGATGAACGACCGCGAATGGCTGAGCGA
ATGGTCGCGCGCGTATGCGGCGAACGCGCTCGCGCTGCTCGTGCTGCTGCCGGCGCTCCTCACGTGGAACGCGCGCGCAT
GCGGCGCGGCGTGGTCGCGCAGCCGCAATCAGTTGCCGGTGCTCGCGACGTTCGGCTCGCTGTGCTTCGCGTCGATATGG
GGATACCAGGCCGAGGTCGCGCGCGCGCTGCTCGCGCTGTCGCTGAGCTGGGCCGCGCTCGAGGGCGGCCTCGTGCTCGC
GTCCCAGCTCAACGCGCTCTCGGCGATCGCCCTGATCGCGATGACGCTCGGCGGGCGCGGCCCGTATGCGTGGCATCACG
ACGGCCACGGCGTCTGGGCGCTGCAGGTCGATCTGATCGGCGTCGCGATCCTGTCGATGTGCATCGCGATCGCGACGGGC
GAGCGGCGGCGGCTTGCGCGGCAGATCGAACGATCGCGCCGTTTCGAGTCGCTCGGCTTTTTCGCCGGCGGAATCGTGCA
CGATTTCAGCAACGTGCTGACGACGATCGATTGCCACGCCGAATACGCGTCCGAGCGGCTCGCGAGCGGCGCGCGCGCGG
ACGATTCGATCGACGAGATTCATCGCGCGGTCGAACGCGGCCGCGATCTGGCGGCGCAGATCCTGCTCGCCGCGCGCCGC
GGCGAGCCCGTGCTGACGCGGGTGAGCGTCGACGAGGTCGTCGACGAGGCGCTCGCCGCGACACGCGCGGCCGCGTGGCG
CGGCGTCGAGATCGTTCGATGCGGCGATGGCGCGCGCGACGGCGCCCGGCGTGGCGACGTTCGTCACGTCGTATTCGCCG
ATCGCAGCCAGCTCGCGCGCGCGGTCATGAATCTCGTCGGCAATGCGGTGCGCGCGGCGCGCGCGACGGTTGTCGTGCGT
GTCGGGGTGAACGGCGGGGTGAATGGCGGCGCGAGCGATGGGGCGAGCCGCGGCGCGAACGACGGCGGCGCGCCGGCCGC
TCGCGCATTCGACGTCGCGATCGGCGAAGCGGCGATGCCGGGCGGCGTGTGGATCGAGGTGAGCGACGACGGCGAAGGCA
TCGACGTCGAGCCGCTCGACCGCATCTTCGAGCCGTTCTATTCGAACCGCTCCGGCGCGCCGGGGACGGGGCTGGGTCTC
GCGATCGTCGCGGGCGCGGCGCTCGCGCACCGCGGGCAGATCGCCGTCGCGACGGATCGGGGCGCGGGCAGTTGCTTCCG
GCTCACGTTGCCGGCGGCATGGGGAACGGGCGCGCCCGGCGCAATGGACGCCGGCGCGCCCGGCGCAGTACAGGAGAGGA
CATGA