Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00016 experimental
T6SS type Type i5
Strain Agrobacterium fabrum str. C58
Replicon (RefSeq) chromosome linear (NC_003063)
Location 1460756..1488734
Function anti-eukaryotic; anti-bacterial; effector translocation
Comment Deletion of hcp results in reduced tumorigenesis on potato tuber slices; Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta; the Tde DNase toxins may be pivotal antibacterial toxins that A. tumefaciens uses against competitors during in planta colonization.
References
[1] Ma LS et al (2012) IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion. J Biol Chem. 287(19):15610-21. [PudMed:22393043]
[2] Lin JS et al (2013) Systematic Dissection of the Agrobacterium Type VI Secretion System Reveals Machinery and Secreted Components for Subcomplex Formation. PLoS One. 8(7):e67647. [PudMed:23861778]
[3] Wu HY et al (2008) Secretome analysis uncovers an Hcp-family protein secreted via a type VI secretion system in Agrobacterium tumefaciens. J Bacteriol. 190(8):2841-50. [PudMed:18263727]
[4] Ma LS et al (2009) An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J Bacteriol. 191(13):4316-29. [PudMed:19395482]
[5] Wu CF et al (2012) Acid-Induced Type VI Secretion System Is Regulated by ExoR-ChvG/ChvI Signaling Cascade in Agrobacterium tumefaciens. PLoS Pathog. 8(9):e1002938. [PudMed:23028331]
[6] Ma LS et al (2014) Agrobacterium tumefaciens Deploys a Superfamily of Type VI Secretion DNase Effectors as Weapons for Interbacterial Competition In Planta. Cell Host Microbe. 16(1):94-104. [PudMed:24981331]
[7] Heckel BC et al (2014) Agrobacterium tumefaciens ExoR Controls Acid Response Genes and Impacts Exopolysaccharide Synthesis, Horizontal Gene Transfer and Virulence Gene Expression. J Bacteriol. 196(18):3221-33. [PudMed:24982308]
[8] Lin JS et al (2014) Fha Interaction with Phosphothreonine of TssL Activates Type VI Secretion in Agrobacterium tumefaciens. PLoS Pathog. 10(3):e1003991. [PudMed:24626341]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHAPAARPpkATagF-PppATagJTssATssBTssCTssDTssETssFTssGTssHTssITssKTssLTssM
Number11111112111112111


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Component ID
Component
Locus tag (Gene)
Coordinate
Strand
Size (bp)
NCBI ID
Product
ATU_RS202651456660..1456956-297WP_006315910.1PRC-barrel domain-containing protein
ATU_RS202701457221..1458357+1137WP_035257912.1polysaccharide export protein
T6CP012426PpkAATU_RS202751458366..1459271-906WP_035257928.1protein kinase
T6CP012427TagF-PppAATU_RS20280 (tagF)1459258..1460673-1416WP_010973754.1type VI secretion system-associated protein TagF
T6CP000114TssMATU_RS20285 (tssM)1460756..1464235-3480WP_010973755.1type VI secretion system membrane subunit TssM
T6CP000115TssLATU_RS20290 (tssL)1464232..1465737-1506WP_010973756.1type VI secretion system protein TssL, long form
T6CP000116TssKATU_RS20295 (tssK)1465734..1467074-1341WP_010973757.1type VI secretion system baseplate subunit TssK
T6CP011404FHAATU_RS203001467064..1468263-1200WP_010973758.1type VI secretion system-associated FHA domain protein
T6CP000117TssGATU_RS20305 (tssG)1468274..1469278-1005WP_010973759.1type VI secretion system baseplate subunit TssG
T6CP000118TssFATU_RS20310 (tssF)1469288..1471069-1782WP_010973760.1type VI secretion system baseplate subunit TssF
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flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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Regulator ID
Locus tag (Gene)
Regulator type
Regulatory pathway
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Target (Regulation)
REG0077 experimentalAtu4335 (Fha/TagH)proteinTppAgrobacterium fabrum str. C58chromosome (NC_003063)1467064..1468263 (-)499280697Unknown (↓)
REG0078 experimentalAtu4330 (PpkA)proteinTppAgrobacterium fabrum str. C58chromosome (NC_003063)1458366..1459271 (-)737274050Unknown (↓)
REG0079 experimentalAtu4331 (TagF-PppA)proteinTppAgrobacterium fabrum str. C58chromosome (NC_003063)1459258..1460673 (-)159186120Fha (Atu4335) (↑)
REG0080 experimentalAtu2091proteinc-di-GMP regulatory pathwayAgrobacterium fabrum str. C58chromosome (NC_003063)2054055..2054864 (-)159185045the levels of c-di-GMP (↑)
REG0081 experimentalAtu5372proteinc-di-GMP regulatory pathwayAgrobacterium fabrum str. C58chromosome (NC_003064)363293..364387 (+)1805892863the levels of c-di-GMP (↑)
REG0082 experimentalAtu1257 (DgcA)proteinc-di-GMP regulatory pathwayAgrobacterium fabrum str. C58chromosome (NC_003062)1244567..1245814 (-)159184686the levels of c-di-GMP (↑)
REG0083 experimentalAtu2179 (DgcC)proteinc-di-GMP regulatory pathwayAgrobacterium fabrum str. C58chromosome (NC_003062)2154605..2155744 (-)159185090the levels of c-di-GMP (↑)
REG0084 experimentalchvGproteinTCS-HKAgrobacterium fabrum str. C58chromosome (NC_003062)34679..36469 (-)chvI (↓)
REG0085 experimentalchvIproteinTCS-RRAgrobacterium fabrum str. C58chromosome (NC_003062)36525..37250 (-)The intergenic region of the imp and hcp operons (↓)
REG0086 experimentalexoRproteinTCS regulatory pathwayAgrobacterium fabrum str. C58chromosome (NC_003062)1702398..1703192 (-)chvG (↑)
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experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Showing 1 to 4 of 4 rows

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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experimental Experimental investigation has been performed.
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Accessory protein ID
Locus tag (Gene)
Type            
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Related structure protein
Related effector
ACP00010 experimentalAtu4349 (tap-1)adaptorAgrobacterium fabrum str. C58chromosome (NC_003063)1483948..1484904 (+)737273750Atu4348 (VgrG1)Atu4350 (Tde1)
ACP00011 experimentalAtu3641adaptorAgrobacterium fabrum str. C58chromosome (NC_003063)693818..694927 (-)499279473Atu3642 (VgrG2)Atu3640 (Tde2)
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