SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00996
Effector type	Tae
Cognate immunity protein	IMU00053 ; IMU00192
Related T6SS (Type)	T6SS00016 (Type i5)
Strain	Agrobacterium fabrum str. C58
Replicon	chromosome
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Description	Tae; amidase
Reference	[1] Lin JS, Ma LS, Lai EM. Systematic dissection of the agrobacterium type VI secretion system reveals machinery and secreted components for subcomplex formation. PLoS One. 2013 Jul 5;8(7):e67647. doi: 10.1371/journal.pone.0067647. Print 2013. PMID: 23861778 [2] Ma LS, Hachani A, Lin JS, et al. Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta. Cell Host Microbe. 2014 Jul 9;16(1):94-104. doi: 10.1016/j.chom.2014.06.002. Epub 2014 Jun 26. PMID: 24981331

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	Atu4347 (Tae4)
Coordinates (Strand)	1480980..1481480 (+)
NCBI ID	159186127
RefSeq	NC_003063
Uniprot ID	Q7CUP8_AGRFC
KEGG ID	atu:Atu4347
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Tae4	PF14113.8	7.8e-19	43..158

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-166	MRVNFDTLYSNYPSSDPSHPNYLSQRDLFTEIGWESFIGNPNYHNTCAIRVSIAFVKSGINIVPSSHRIQKGPYAGKGIEVNMRRLATLMKRTSYLGEPDPYTPATARNGIGARNGVVAFNNIPGYTGGGHIDLVRGGSEATQCASACYYNSETIWFWPLQASRGS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9829	-	-

Protein sequence: 167 a.a. .

                                    >EFF00996 NC_003063:1480980-1481480 Type VI Amidase Effector (Tae) [Agrobacterium fabrum str. C58]
MRVNFDTLYSNYPSSDPSHPNYLSQRDLFTEIGWESFIGNPNYHNTCAIRVSIAFVKSGINIVPSSHRIQKGPYAGKGIE
VNMRRLATLMKRTSYLGEPDPYTPATARNGIGARNGVVAFNNIPGYTGGGHIDLVRGGSEATQCASACYYNSETIWFWPL
QASRGS*

                                

Nucleotide sequence: 501 bp .

                                    >EFF00996 NC_003063:1480980-1481480 Type VI Amidase Effector (Tae) [Agrobacterium fabrum str. C58]
ATGCGCGTTAACTTTGACACCCTCTATAGCAATTATCCGAGCAGCGATCCGTCGCATCCGAATTATCTCAGCCAGCGTGA
TCTGTTCACGGAAATCGGCTGGGAGAGCTTCATCGGCAATCCGAACTATCATAATACCTGCGCCATCCGCGTCAGCATCG
CTTTCGTGAAGTCGGGCATCAACATCGTGCCCTCTTCGCACCGCATCCAGAAGGGCCCCTATGCCGGAAAGGGCATCGAG
GTGAACATGCGCCGCCTTGCCACCCTGATGAAGCGGACGAGCTATCTCGGCGAGCCCGATCCTTACACGCCCGCCACCGC
CCGCAACGGCATCGGCGCGCGCAACGGTGTCGTGGCCTTCAACAATATTCCCGGCTACACCGGTGGCGGTCATATCGATC
TCGTGCGCGGCGGGTCGGAAGCCACCCAATGCGCGTCGGCCTGCTACTACAATTCGGAAACGATCTGGTTCTGGCCGCTG
CAGGCCAGCCGCGGGTCCTGA

                                