Detailed information of regulator


Summary

Regulator ID REG0084 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-HK
KEGG pathway (determined by BlastKOALA) Two-component system (map02020)
Related T6SS T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Sequence Protein sequence (597 a.a.); Nucleotide sequence (1791 bp)
Description The proteins encoded by the imp or hcp operon reduced with chvG or chvI deletion as determined by western blot and qRT-PCR analyses.
Reference
External database links

Locus tag (Gene) chvG
Coordinates (Strand) 34679..36469 (-)
NCBI ID
RefSeq NC_003062
Uniprot ID -
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02020
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
chvI ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Stimulus_sens_1 PF13756.8 2.4e-35 132..241
Sensor_TM1 PF13755.8 3e-28 33..97
HATPase_c PF02518.28 1.1e-21 476..590
HisKA PF00512.27 9.8e-15 360..421
HAMP PF00672.27 3e-08 298..352
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-47 VLKKTPETVSDSDDAEERGSERRHRIHPLTIIRRIFGNAVFSSLTRR
Transmembrane helix 48-67 ILFFNVAATVVLVGGILYLN
Inside 68-283 QFREGLIDARVESLLTQGEIIAGAVSASASVDTNSITINPEKLLELQAGQSITPAPNDEDLSFPINPERVAPVLRRLISPTRTRARLFDADANLLLDSRHLYSRGQVLRFDLPPVTPETQTWGDWFTSMFNRMLQPSSLPQYKEAPGGDGSIYPEVMNALTGVRGAVVRVTEKGELIVSVAVPVQRFRAVLGVLLLSTQAGDIDKIVHAERLAIMR
Transmembrane helix 284-306 VFGIATLVNIVLSLLLSSTIATP
Outside 307-596 LRRLSAAAIRVRRGARTREEIPDFSARQDEIGNLSIALREMTTALYDRIDAIESFAADVSHELKNPLTSLRSAVETLPRAKTEESKQRLTEIIFHDVRRLDRLISDISDASRLDAELARADASPLDLDVLMKGLVDISRQISTKKKSVAIDYVADRKAGAKTSFVVNGHDLRIGQIVTNLIENARSFVSEESGRITVRLSRHKDRCIVQVEDNGPGIQAEDIDRIFERFYTDRPASEGFGQNSGLGLSISRQIAEAHGGSLRAENVVDKYGVISGARFTLSLPAAETHER


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9560 - -


  Protein sequence of regulator: 597 a.a.    .

>REG0084 NC_003062:c36469-34679 [Agrobacterium fabrum str. C58]
VLKKTPETVSDSDDAEERGSERRHRIHPLTIIRRIFGNAVFSSLTRRILFFNVAATVVLVGGILYLNQFREGLIDARVES
LLTQGEIIAGAVSASASVDTNSITINPEKLLELQAGQSITPAPNDEDLSFPINPERVAPVLRRLISPTRTRARLFDADAN
LLLDSRHLYSRGQVLRFDLPPVTPETQTWGDWFTSMFNRMLQPSSLPQYKEAPGGDGSIYPEVMNALTGVRGAVVRVTEK
GELIVSVAVPVQRFRAVLGVLLLSTQAGDIDKIVHAERLAIMRVFGIATLVNIVLSLLLSSTIATPLRRLSAAAIRVRRG
ARTREEIPDFSARQDEIGNLSIALREMTTALYDRIDAIESFAADVSHELKNPLTSLRSAVETLPRAKTEESKQRLTEIIF
HDVRRLDRLISDISDASRLDAELARADASPLDLDVLMKGLVDISRQISTKKKSVAIDYVADRKAGAKTSFVVNGHDLRIG
QIVTNLIENARSFVSEESGRITVRLSRHKDRCIVQVEDNGPGIQAEDIDRIFERFYTDRPASEGFGQNSGLGLSISRQIA
EAHGGSLRAENVVDKYGVISGARFTLSLPAAETHER*

  Nucleotide sequence of regulator: 1791 bp    .

>REG0084 NC_003062:c36469-34679 [Agrobacterium fabrum str. C58]
GTGTTGAAGAAAACGCCGGAAACCGTCTCGGACAGTGACGATGCCGAAGAACGCGGCAGCGAACGCCGCCATCGTATCCA
TCCGCTGACGATCATCCGGCGCATTTTCGGCAATGCGGTGTTTTCCAGCCTCACGCGCCGTATCCTCTTCTTCAATGTCG
CCGCGACCGTGGTTCTGGTCGGCGGCATTCTCTACCTCAACCAGTTCCGTGAAGGGCTGATCGACGCGCGCGTGGAAAGC
CTGTTGACGCAGGGCGAAATCATCGCCGGCGCGGTCTCCGCTTCCGCATCGGTCGATACCAACTCGATCACCATCAATCC
GGAAAAACTGCTCGAATTGCAGGCGGGGCAGAGCATCACCCCTGCCCCCAACGACGAGGATCTGAGTTTCCCGATCAACC
CGGAGCGAGTGGCGCCTGTGCTGCGGCGTCTGATTTCGCCGACACGCACGCGCGCGCGGCTGTTCGATGCCGATGCCAAT
CTGTTGCTCGATTCCCGCCACCTTTATTCACGCGGACAGGTTCTGCGCTTCGATCTGCCGCCGGTGACGCCGGAAACCCA
GACTTGGGGTGACTGGTTCACCAGCATGTTCAACCGCATGCTGCAGCCGAGCAGCCTGCCGCAATACAAGGAAGCGCCGG
GTGGCGATGGCTCGATCTATCCAGAAGTGATGAATGCGCTGACGGGGGTTCGCGGCGCGGTCGTGCGCGTGACCGAAAAG
GGTGAGCTGATCGTTTCGGTCGCCGTGCCGGTTCAGCGCTTCCGGGCCGTACTAGGCGTATTGCTTTTGTCCACCCAGGC
GGGAGATATCGACAAGATCGTGCATGCCGAGCGCCTTGCCATCATGCGCGTCTTCGGCATCGCCACGCTGGTCAATATCG
TGTTGTCACTGCTTTTATCCTCCACCATCGCCACGCCGCTAAGACGCCTTTCGGCGGCCGCGATCCGCGTGCGCCGCGGG
GCGAGAACGCGTGAGGAAATACCGGATTTCTCAGCGCGTCAGGACGAGATCGGAAACCTCTCCATAGCGTTGCGTGAAAT
GACGACGGCGCTCTACGACCGTATCGACGCCATCGAAAGTTTCGCCGCCGACGTCAGCCATGAACTCAAGAACCCGCTGA
CCTCGCTGCGCAGCGCCGTGGAAACCCTGCCACGCGCCAAGACCGAAGAGTCCAAACAGCGGCTGACCGAAATCATCTTC
CATGACGTTCGGCGCCTCGATCGCCTGATCAGCGATATTTCGGACGCGTCGCGCCTCGATGCCGAGCTGGCGCGCGCCGA
TGCTTCGCCACTCGACCTCGATGTGCTGATGAAGGGTCTGGTGGACATATCCCGCCAGATCAGCACCAAGAAAAAGAGCG
TGGCGATCGATTATGTCGCCGACCGGAAGGCCGGAGCCAAAACCTCTTTCGTAGTCAACGGCCACGATCTTCGTATCGGG
CAGATTGTCACCAACCTTATCGAGAATGCACGTTCTTTCGTTTCGGAGGAAAGCGGCCGCATCACCGTGCGCCTTTCCCG
CCACAAGGACCGCTGCATCGTGCAGGTGGAAGACAACGGCCCCGGCATTCAGGCAGAAGATATCGACCGAATTTTCGAGC
GGTTTTATACAGACCGGCCGGCCAGCGAAGGTTTCGGCCAGAATTCCGGCCTTGGCCTTTCCATCAGCCGCCAGATCGCG
GAAGCCCATGGCGGCAGCCTCCGGGCGGAAAACGTGGTCGACAAATACGGCGTTATATCAGGCGCGCGCTTCACCCTGTC
GCTGCCGGCGGCCGAAACCCATGAACGCTGA