Detailed information of regulator


Summary

Regulator ID REG0084 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-HK
KEGG pathway (determined by BlastKOALA) Two-component system (map02020)
Related T6SS T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Sequence Protein sequence (597 a.a.); Nucleotide sequence (1791 bp)
Description The proteins encoded by the imp or hcp operon reduced with chvG or chvI deletion as determined by western blot and qRT-PCR analyses.
Reference
External database links

Locus tag (Gene) chvG
Coordinates (Strand) 34679..36469 (-)
NCBI ID
RefSeq NC_003062
Uniprot ID -
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02020
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
chvI↓-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
Stimulus_sens_1PF13756.82.4e-35132..241
Sensor_TM1PF13755.83e-2833..97
HATPase_cPF02518.281.1e-21476..590
HisKAPF00512.279.8e-15360..421
HAMPPF00672.273e-08298..352
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-47VLKKTPETVSDSDDAEERGSERRHRIHPLTIIRRIFGNAVFSSLTRR
Transmembrane helix48-67ILFFNVAATVVLVGGILYLN
Inside68-283QFREGLIDARVESLLTQGEIIAGAVSASASVDTNSITINPEKLLELQAGQSITPAPNDEDLSFPINPERVAPVLRRLISPTRTRARLFDADANLLLDSRHLYSRGQVLRFDLPPVTPETQTWGDWFTSMFNRMLQPSSLPQYKEAPGGDGSIYPEVMNALTGVRGAVVRVTEKGELIVSVAVPVQRFRAVLGVLLLSTQAGDIDKIVHAERLAIMR
Transmembrane helix284-306VFGIATLVNIVLSLLLSSTIATP
Outside307-596LRRLSAAAIRVRRGARTREEIPDFSARQDEIGNLSIALREMTTALYDRIDAIESFAADVSHELKNPLTSLRSAVETLPRAKTEESKQRLTEIIFHDVRRLDRLISDISDASRLDAELARADASPLDLDVLMKGLVDISRQISTKKKSVAIDYVADRKAGAKTSFVVNGHDLRIGQIVTNLIENARSFVSEESGRITVRLSRHKDRCIVQVEDNGPGIQAEDIDRIFERFYTDRPASEGFGQNSGLGLSISRQIAEAHGGSLRAENVVDKYGVISGARFTLSLPAAETHER


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9560--


  Protein sequence of regulator: 597 a.a.    .

>REG0084 NC_003062:c36469-34679 [Agrobacterium fabrum str. C58]
VLKKTPETVSDSDDAEERGSERRHRIHPLTIIRRIFGNAVFSSLTRRILFFNVAATVVLVGGILYLNQFREGLIDARVES
LLTQGEIIAGAVSASASVDTNSITINPEKLLELQAGQSITPAPNDEDLSFPINPERVAPVLRRLISPTRTRARLFDADAN
LLLDSRHLYSRGQVLRFDLPPVTPETQTWGDWFTSMFNRMLQPSSLPQYKEAPGGDGSIYPEVMNALTGVRGAVVRVTEK
GELIVSVAVPVQRFRAVLGVLLLSTQAGDIDKIVHAERLAIMRVFGIATLVNIVLSLLLSSTIATPLRRLSAAAIRVRRG
ARTREEIPDFSARQDEIGNLSIALREMTTALYDRIDAIESFAADVSHELKNPLTSLRSAVETLPRAKTEESKQRLTEIIF
HDVRRLDRLISDISDASRLDAELARADASPLDLDVLMKGLVDISRQISTKKKSVAIDYVADRKAGAKTSFVVNGHDLRIG
QIVTNLIENARSFVSEESGRITVRLSRHKDRCIVQVEDNGPGIQAEDIDRIFERFYTDRPASEGFGQNSGLGLSISRQIA
EAHGGSLRAENVVDKYGVISGARFTLSLPAAETHER*

  Nucleotide sequence of regulator: 1791 bp    .

>REG0084 NC_003062:c36469-34679 [Agrobacterium fabrum str. C58]
GTGTTGAAGAAAACGCCGGAAACCGTCTCGGACAGTGACGATGCCGAAGAACGCGGCAGCGAACGCCGCCATCGTATCCA
TCCGCTGACGATCATCCGGCGCATTTTCGGCAATGCGGTGTTTTCCAGCCTCACGCGCCGTATCCTCTTCTTCAATGTCG
CCGCGACCGTGGTTCTGGTCGGCGGCATTCTCTACCTCAACCAGTTCCGTGAAGGGCTGATCGACGCGCGCGTGGAAAGC
CTGTTGACGCAGGGCGAAATCATCGCCGGCGCGGTCTCCGCTTCCGCATCGGTCGATACCAACTCGATCACCATCAATCC
GGAAAAACTGCTCGAATTGCAGGCGGGGCAGAGCATCACCCCTGCCCCCAACGACGAGGATCTGAGTTTCCCGATCAACC
CGGAGCGAGTGGCGCCTGTGCTGCGGCGTCTGATTTCGCCGACACGCACGCGCGCGCGGCTGTTCGATGCCGATGCCAAT
CTGTTGCTCGATTCCCGCCACCTTTATTCACGCGGACAGGTTCTGCGCTTCGATCTGCCGCCGGTGACGCCGGAAACCCA
GACTTGGGGTGACTGGTTCACCAGCATGTTCAACCGCATGCTGCAGCCGAGCAGCCTGCCGCAATACAAGGAAGCGCCGG
GTGGCGATGGCTCGATCTATCCAGAAGTGATGAATGCGCTGACGGGGGTTCGCGGCGCGGTCGTGCGCGTGACCGAAAAG
GGTGAGCTGATCGTTTCGGTCGCCGTGCCGGTTCAGCGCTTCCGGGCCGTACTAGGCGTATTGCTTTTGTCCACCCAGGC
GGGAGATATCGACAAGATCGTGCATGCCGAGCGCCTTGCCATCATGCGCGTCTTCGGCATCGCCACGCTGGTCAATATCG
TGTTGTCACTGCTTTTATCCTCCACCATCGCCACGCCGCTAAGACGCCTTTCGGCGGCCGCGATCCGCGTGCGCCGCGGG
GCGAGAACGCGTGAGGAAATACCGGATTTCTCAGCGCGTCAGGACGAGATCGGAAACCTCTCCATAGCGTTGCGTGAAAT
GACGACGGCGCTCTACGACCGTATCGACGCCATCGAAAGTTTCGCCGCCGACGTCAGCCATGAACTCAAGAACCCGCTGA
CCTCGCTGCGCAGCGCCGTGGAAACCCTGCCACGCGCCAAGACCGAAGAGTCCAAACAGCGGCTGACCGAAATCATCTTC
CATGACGTTCGGCGCCTCGATCGCCTGATCAGCGATATTTCGGACGCGTCGCGCCTCGATGCCGAGCTGGCGCGCGCCGA
TGCTTCGCCACTCGACCTCGATGTGCTGATGAAGGGTCTGGTGGACATATCCCGCCAGATCAGCACCAAGAAAAAGAGCG
TGGCGATCGATTATGTCGCCGACCGGAAGGCCGGAGCCAAAACCTCTTTCGTAGTCAACGGCCACGATCTTCGTATCGGG
CAGATTGTCACCAACCTTATCGAGAATGCACGTTCTTTCGTTTCGGAGGAAAGCGGCCGCATCACCGTGCGCCTTTCCCG
CCACAAGGACCGCTGCATCGTGCAGGTGGAAGACAACGGCCCCGGCATTCAGGCAGAAGATATCGACCGAATTTTCGAGC
GGTTTTATACAGACCGGCCGGCCAGCGAAGGTTTCGGCCAGAATTCCGGCCTTGGCCTTTCCATCAGCCGCCAGATCGCG
GAAGCCCATGGCGGCAGCCTCCGGGCGGAAAACGTGGTCGACAAATACGGCGTTATATCAGGCGCGCGCTTCACCCTGTC
GCTGCCGGCGGCCGAAACCCATGAACGCTGA