Detailed information of component protein


Summary

Component ID T6CP000118
Component protein type TssF
T6SS ID (Type) T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome linear
Sequence Protein sequence (594 a.a.); Nucleotide sequence (1782 bp)
Reference
[1] Ma LS, Narberhaus F, Lai EM. IcmF family protein TssM exhibits ATPase activity and energizes type VI secretion. J Biol Chem. 2012 May 4;287(19):15610-21. doi: 10.1074/jbc.M111.301630. Epub 2012 Mar 5. PMID: 22393043
[2] Wu HY, Chung PC, Shih HW, et al. Secretome analysis uncovers an Hcp-family protein secreted via a type VI secretion system in Agrobacterium tumefaciens. J Bacteriol. 2008 Apr;190(8):2841-50. doi: 10.1128/JB.01775-07. Epub 2008 Feb 8. PMID: 18263727
[3] Wu CF, Lin JS, Shaw GC, et al. Acid-induced type VI secretion system is regulated by ExoR-ChvG/ChvI signaling cascade in Agrobacterium tumefaciens. PLoS Pathog. 2012 Sep;8(9):e1002938. doi: 10.1371/journal.ppat.1002938. Epub 2012 Sep 27. PMID: 23028331
[4] Lin JS, Ma LS, Lai EM. Systematic dissection of the agrobacterium type VI secretion system reveals machinery and secreted components for subcomplex formation. PLoS One. 2013 Jul 5;8(7):e67647. doi: 10.1371/journal.pone.0067647. Print 2013. PMID: 23861778
[5] Ma LS, Hachani A, Lin JS, et al. Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta. Cell Host Microbe. 2014 Jul 9;16(1):94-104. doi: 10.1016/j.chom.2014.06.002. Epub 2014 Jun 26. PMID: 24981331
[6] Heckel BC, Tomlinson AD, Morton ER, et al. Agrobacterium tumefaciens exoR controls acid response genes and impacts exopolysaccharide synthesis, horizontal gene transfer, and virulence gene expression. J Bacteriol. 2014 Sep;196(18):3221-33. doi: 10.1128/JB.01751-14. Epub 2014 Jun 30. PMID: 24982308
[7] Lin JS, Wu HH, Hsu PH, et al. Fha interaction with phosphothreonine of TssL activates type VI secretion in Agrobacterium tumefaciens. PLoS Pathog. 2014 Mar 13;10(3):e1003991. doi: 10.1371/journal.ppat.1003991. eCollection 2014 Mar. PMID: 24626341
[8] Heckel BC, Tomlinson AD, Morton ER, et al. Agrobacterium tumefaciens exoR controls acid response genes and impacts exopolysaccharide synthesis, horizontal gene transfer, and virulence gene expression. J Bacteriol. 2014 Sep;196(18):3221-33. doi: 10.1128/JB.01751-14. Epub 2014 Jun 30. PMID: 24982308
[9] Ma LS, Lin JS, Lai EM. An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens. J Bacteriol. 2009 Jul;191(13):4316-29. doi: 10.1128/JB.00029-09. Epub 2009 Apr 24. PMID: 19395482
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ATU_RS20310
Coordinate (Strand) 1469288..1471069 (-)
NCBI ID WP_010973760.1
RefSeq NC_003063
Uniprot ID A0A7Y0T2I9_9HYPH, A9CGG3_AGRFC, A0A083ZIC4_RHIRD
KEGG ID atu:Atu4337
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 4e-201 1..590
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-593 MADGFLEKYNDELFALRKRASRFAAAFPKIAGRLRMTGDVADDPHVERLIQSFAYSAARVRQKLDDEFPELSDSLLETLYPHYLAPIPSMSVVQFAPSPALATVQTLARHSEILAEPVGGESCRFRTTQEVQIAPLQIAAATLAGQPIDAPFSASFAGTASCLRLSLRSTAPRGGTFPEMGLTKLQVFLSAAWQQATALYELLTNHCVGMALARHSEDKEAIFLPAGNLRPSGFTRDQAMLPYPANSFDGYRLLTEFFALPQKFLFLDIDGLHRWSGGDCELYIYLDAADAQLERMVSVKDFVLNASPVINLFKQSCEPLSLDGTRTEYRLLPDARRQRTREIYAVEDVQLTSRSGQSEKTSPFFGRTQRNNGANVFWQIQRRFDDDGSSDTDIAFVDEKRGPLGPLDVVASIDTLCINRELPSQLPFGGGHPFLQLSTGNEAVKSVHALMPPTAAIRVNERSAREWRLISHLLLNHLSLTDSGGAPLKDILSLYSFRDSPETRQLVEAISKVEAQNSHARIGATMVPGTDITVEFDPALIARPAAFVFAGVLNHFFGLYTSINSFTRLTATMRGHSKPIARWPARAADRPLL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9844 - -


  Protein sequence: 594 a.a.    

>T6CP000118 NC_003063:c1471069-1469288 [Agrobacterium fabrum str. C58] [TssF]
MADGFLEKYNDELFALRKRASRFAAAFPKIAGRLRMTGDVADDPHVERLIQSFAYSAARVRQKLDDEFPELSDSLLETLY
PHYLAPIPSMSVVQFAPSPALATVQTLARHSEILAEPVGGESCRFRTTQEVQIAPLQIAAATLAGQPIDAPFSASFAGTA
SCLRLSLRSTAPRGGTFPEMGLTKLQVFLSAAWQQATALYELLTNHCVGMALARHSEDKEAIFLPAGNLRPSGFTRDQAM
LPYPANSFDGYRLLTEFFALPQKFLFLDIDGLHRWSGGDCELYIYLDAADAQLERMVSVKDFVLNASPVINLFKQSCEPL
SLDGTRTEYRLLPDARRQRTREIYAVEDVQLTSRSGQSEKTSPFFGRTQRNNGANVFWQIQRRFDDDGSSDTDIAFVDEK
RGPLGPLDVVASIDTLCINRELPSQLPFGGGHPFLQLSTGNEAVKSVHALMPPTAAIRVNERSAREWRLISHLLLNHLSL
TDSGGAPLKDILSLYSFRDSPETRQLVEAISKVEAQNSHARIGATMVPGTDITVEFDPALIARPAAFVFAGVLNHFFGLY
TSINSFTRLTATMRGHSKPIARWPARAADRPLL*

  Nucleotide sequence: 1782 bp    

>T6CP000118 NC_003063:c1471069-1469288 [Agrobacterium fabrum str. C58] [TssF]
ATGGCTGACGGCTTCCTCGAAAAATACAATGACGAGCTTTTTGCGCTGCGAAAACGCGCTTCGCGCTTTGCGGCGGCGTT
TCCGAAGATCGCTGGCAGGCTGCGCATGACTGGTGATGTCGCCGACGATCCGCATGTCGAGCGGCTGATCCAGAGCTTTG
CCTATTCCGCCGCCCGCGTGCGCCAAAAGCTGGACGACGAATTTCCCGAATTGTCAGACAGCCTGCTGGAAACGCTCTAT
CCGCATTATCTTGCGCCGATCCCTTCGATGAGCGTCGTGCAATTCGCTCCGAGTCCGGCGCTGGCAACCGTACAGACGCT
TGCCCGTCACAGCGAAATCCTCGCCGAACCCGTGGGCGGCGAAAGCTGCCGTTTCCGCACCACGCAGGAGGTGCAGATCG
CGCCGCTGCAGATCGCGGCAGCGACGCTTGCCGGCCAGCCGATCGACGCGCCTTTTTCGGCATCCTTTGCCGGCACTGCC
AGTTGCCTGCGGCTTTCGCTGCGCAGCACGGCGCCGCGCGGCGGCACATTCCCGGAAATGGGACTGACGAAACTCCAGGT
CTTCCTGTCCGCTGCATGGCAGCAGGCAACGGCGCTTTACGAGCTTTTGACCAACCATTGCGTCGGCATGGCGCTTGCCC
GTCATTCCGAGGACAAGGAAGCCATCTTCCTGCCGGCCGGCAATCTCAGACCTTCCGGGTTCACTCGCGATCAGGCCATG
TTGCCCTATCCGGCCAACAGCTTTGACGGATACAGGCTTCTCACCGAATTCTTCGCGCTGCCGCAGAAGTTTCTCTTTCT
CGATATTGACGGGCTGCACAGATGGAGCGGCGGTGATTGCGAGCTTTATATCTACCTGGATGCCGCCGATGCGCAGCTTG
AGCGCATGGTGTCGGTCAAGGATTTCGTGCTGAACGCGTCTCCGGTCATCAACCTCTTCAAGCAAAGCTGCGAACCGCTC
AGCCTAGATGGAACCCGGACCGAATACCGCCTGCTGCCGGATGCACGCCGCCAGCGGACCCGCGAGATATACGCGGTCGA
AGACGTCCAGCTGACCAGCCGTTCCGGGCAGAGCGAGAAAACCTCTCCCTTCTTCGGCCGCACCCAACGCAACAATGGTG
CGAATGTGTTCTGGCAGATCCAGCGCCGGTTCGACGATGATGGCTCCTCCGATACCGACATCGCCTTCGTGGATGAAAAG
CGCGGACCGCTGGGGCCGCTCGACGTGGTAGCGAGCATCGACACGCTCTGCATCAACCGCGAGCTGCCGAGCCAGCTTCC
GTTTGGTGGTGGGCACCCCTTCCTGCAACTTTCGACCGGCAATGAAGCGGTGAAATCGGTCCATGCGCTGATGCCGCCGA
CGGCGGCGATCCGCGTCAACGAACGCTCGGCGCGCGAGTGGCGGCTGATATCGCATCTTCTCCTCAACCATCTTTCCCTC
ACCGATAGCGGCGGCGCTCCGCTGAAAGATATTCTGTCGCTTTATTCCTTCAGAGACAGCCCGGAAACCCGGCAGCTCGT
GGAGGCGATCAGCAAGGTCGAAGCCCAGAATTCCCACGCCCGCATCGGCGCGACGATGGTACCCGGCACCGACATAACGG
TGGAGTTCGATCCTGCGCTGATCGCACGGCCTGCGGCCTTTGTATTCGCCGGCGTCCTCAACCATTTCTTCGGGCTTTAC
ACCTCGATCAACAGCTTTACGCGGCTGACGGCGACGATGCGTGGCCATTCCAAACCAATTGCCCGCTGGCCGGCCCGCGC
CGCTGACCGGCCGTTGCTTTAG