Detailed information of regulator


Summary

Regulator ID REG0079 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00016 experimental (Type i5)
Strain Agrobacterium fabrum str. C58
Replicon chromosome
Sequence Protein sequence (472 a.a.); Nucleotide sequence (1416 bp)
Description The TagF–Fha interaction has a different impact in different bacteria but ultimately prevents successful T6SS assembly.
Reference
External database links

Locus tag (Gene) Atu4331 (TagF-PppA)
Coordinates (Strand) 1459258..1460673 (-)
NCBI ID 159186120
RefSeq NC_003063
Uniprot ID Q7CUN2_AGRFC
KEGG ID atu:Atu4331
KEGG pathway (determined by BlastKOALA) map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Fha (Atu4335) ↑ 15890648
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
TagF_N PF09867.11 7.4e-42 19..153
PP2C PF00481.23 7.3e-05 275..380
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-471 MADQASRSTQTAAESDRIGFFGKVPSHGDFISDGLERELIATFDDWMRSGMHACADMFAGRWTEIFSSSPPIRFIVESGIWGNCAYAGVLVPSADRVGRKYPLAIIAQLNGFRRHPRTLYLDDTWFMAVEALAETSMTGDFDVSRFNASIKKLRLPKPRDEDDAPEMVRGRAPTSLWWHIDPVSRRARGLKFDGKPRASDFLRLFSDMTGKGDEDGSRAAPAPATVQEKTKQQIPPVTPAPAHPAEQPSSVIYSYATHPGTRLSLNADALFVSQTPSLFAIADGQGDTHSAAEAARLATNILAETPDAENPEMMAQQIRGKLGTVNSLLLSRQATGADGRPMASVVIASIIAHRLSVLWSGDARAYLLKNGTMHQLSRDHVVVGMKKQLSQCVGLSQQFRPDIVFDEWGLQDRLLLCSYPLVQILRERAIAEIVFNTPIKDCANALVQEALIENARENVSAIVIGNRREQG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9792 - -


  Protein sequence of regulator: 472 a.a.    .

>REG0079 NC_003063:c1460673-1459258 [Agrobacterium fabrum str. C58]
MADQASRSTQTAAESDRIGFFGKVPSHGDFISDGLERELIATFDDWMRSGMHACADMFAGRWTEIFSSSPPIRFIVESGI
WGNCAYAGVLVPSADRVGRKYPLAIIAQLNGFRRHPRTLYLDDTWFMAVEALAETSMTGDFDVSRFNASIKKLRLPKPRD
EDDAPEMVRGRAPTSLWWHIDPVSRRARGLKFDGKPRASDFLRLFSDMTGKGDEDGSRAAPAPATVQEKTKQQIPPVTPA
PAHPAEQPSSVIYSYATHPGTRLSLNADALFVSQTPSLFAIADGQGDTHSAAEAARLATNILAETPDAENPEMMAQQIRG
KLGTVNSLLLSRQATGADGRPMASVVIASIIAHRLSVLWSGDARAYLLKNGTMHQLSRDHVVVGMKKQLSQCVGLSQQFR
PDIVFDEWGLQDRLLLCSYPLVQILRERAIAEIVFNTPIKDCANALVQEALIENARENVSAIVIGNRREQG*

  Nucleotide sequence of regulator: 1416 bp    .

>REG0079 NC_003063:c1460673-1459258 [Agrobacterium fabrum str. C58]
ATGGCCGATCAGGCATCAAGGTCCACGCAAACAGCGGCGGAAAGCGATCGCATCGGTTTCTTCGGCAAGGTCCCCAGCCA
TGGCGATTTCATTTCCGATGGGCTCGAGCGGGAATTGATCGCTACCTTCGACGACTGGATGCGATCCGGCATGCACGCCT
GCGCGGATATGTTCGCAGGCCGATGGACAGAGATATTCTCGTCGTCGCCGCCCATACGCTTCATCGTCGAAAGCGGCATA
TGGGGAAACTGCGCCTATGCCGGCGTGCTGGTGCCGAGCGCCGACCGGGTGGGCCGTAAATATCCGCTTGCCATCATTGC
CCAGCTCAACGGCTTCCGCAGACATCCGCGAACACTCTATCTCGACGACACCTGGTTCATGGCCGTAGAGGCATTGGCCG
AGACATCGATGACCGGCGATTTCGACGTGTCCCGGTTTAATGCCTCGATCAAGAAATTGCGCCTACCGAAGCCGCGGGAC
GAGGATGACGCGCCGGAAATGGTCCGCGGCCGCGCGCCGACAAGCCTCTGGTGGCACATCGATCCCGTCAGCCGCCGTGC
ACGCGGCCTCAAATTCGACGGCAAGCCGAGAGCATCCGATTTTCTGCGCCTCTTCAGCGACATGACAGGCAAAGGCGACG
AGGACGGTTCAAGAGCAGCGCCGGCGCCGGCAACGGTGCAGGAGAAGACAAAACAGCAAATACCGCCCGTGACACCCGCG
CCAGCCCACCCAGCGGAACAGCCGTCATCTGTCATCTACAGCTATGCCACACATCCGGGAACGCGGCTTTCCCTGAATGC
GGATGCACTGTTCGTTTCCCAGACGCCTTCCCTCTTCGCCATAGCGGATGGACAAGGCGATACGCATAGCGCCGCAGAGG
CCGCGCGCCTCGCCACGAATATTCTTGCTGAAACGCCAGATGCCGAAAACCCGGAGATGATGGCACAGCAGATCAGGGGA
AAGCTCGGCACCGTCAACAGCCTGCTCCTCTCCCGTCAGGCGACTGGTGCCGATGGCAGGCCGATGGCCAGCGTCGTCAT
AGCCTCGATCATCGCCCACAGGCTGAGCGTCCTGTGGTCCGGTGATGCGCGCGCCTATCTCCTGAAAAACGGCACCATGC
ACCAGCTTTCGCGCGATCACGTCGTTGTCGGCATGAAAAAACAGCTCTCGCAATGTGTCGGGCTTTCCCAGCAATTCCGG
CCGGATATCGTCTTCGATGAGTGGGGTTTGCAGGACAGGCTACTGCTGTGCAGCTACCCGCTCGTACAAATCCTCCGAGA
ACGCGCCATTGCCGAGATCGTCTTCAACACCCCGATCAAGGATTGCGCCAATGCCCTGGTCCAGGAAGCGCTGATCGAAA
ACGCCCGTGAGAATGTCAGCGCCATCGTGATTGGAAACCGGCGTGAGCAAGGCTAG