Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0006	L085_RS13655 (ppka)	T6SS01033	protein	Tpp	Serratia sp. FS14	chromosome (NZ_CP005927)	3028273..3029718 (-)	763151328	Unknown (↓)
REG0014	SMDB11_2275 (PpKA)	T6SS00929	protein	Tpp	Serratia marcescens subsp. marcescens Db11	chromosome (HG326223)	2399154..2400599 (+)	560174118	SMDB11_2267 (FHA) (↓)
REG0015	SMDB11_2268 (PppA)	T6SS00929	protein	Tpp	Serratia marcescens subsp. marcescens Db11	chromosome (HG326223)	2390232..2391005 (+)	560174111	SMDB11_2267 (FHA) (↑)
REG0016	SMDB11_2269 (RtkS)	T6SS00929	protein	Tpp	Serratia marcescens subsp. marcescens Db11	chromosome (HG326223)	2391034..2392020 (+)	560174112	SMDB11_2275 (PpKA) (↓)
REG0017	SMDB11_2256 (TagF)	T6SS00929	protein	Tpp	Serratia marcescens subsp. marcescens Db11	chromosome (HG326223)	2380702..2381421 (+)	560174099	Unknown (↑)
REG0042	PA0075 (PppA)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	90106..90834 (-)	15595273	PA0081 (Fha-1/TagH) (↑)
REG0043	PA0074 (PpkA)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	87000..90098 (-)	15595272	PA0081 (Fha-1/TagH) (↓)
REG0047	PA0072 (TagS)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	85085..86284 (-)	15595270	PA0073 (TagT) (↓)
REG0048	PA0070 (TagQ)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	82404..83318 (-)	15595268	PA0071 (tagR) (↓)
REG0049	PA0073 (TagT)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	86284..87003 (-)	15595271	PA0072 (TagS) (↓)
REG0052	PA0071 (tagR)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	83380..85092 (-)	15595269	PA0074 (PpkA) (↓)
REG0056	PA0076 (TagF)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	90844..91524 (-)	15595274	hcp1 (PA0086) secretion (↑)
REG0057	PA0081 (Fha-1/TagH)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	98263..99756 (-)	15595279	Unknown (↓); clpV (↓)
REG0077	Atu4335 (Fha/TagH)	T6SS00016	protein	Tpp	Agrobacterium fabrum str. C58	chromosome (NC_003063)	1467064..1468263 (-)	499280697	Unknown (↓)
REG0078	Atu4330 (PpkA)	T6SS00016	protein	Tpp	Agrobacterium fabrum str. C58	chromosome (NC_003063)	1458366..1459271 (-)	737274050	Unknown (↓)
REG0079	Atu4331 (TagF-PppA)	T6SS00016	protein	Tpp	Agrobacterium fabrum str. C58	chromosome (NC_003063)	1459258..1460673 (-)	159186120	Fha (Atu4335) (↑)
REG0092	PFL_RS30815 (pppA)	T6SS00035	protein	Tpp	Pseudomonas protegens Pf-5	chromosome (NC_004129)	6902924..6903658 (-)	499376669	Hcp secretion (↑)
REG0257	VEPGS_0614 (PppA)	T6SS00794	protein	Tpp	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	15765..16880 (+)	225382180	LuxR (↓)
REG0258	VEPGS_0606 (PpkA2)	T6SS00794	protein	Tpp	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	3227..5377 (-)	225382172	LuxR (↓); VEPGS_0610 (DotU2) (↓)
REG0259	VEPGS_0607 (Fha2)	T6SS00794	protein	Tpp	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	5690..6775 (+)	225382173	VEPGS_0610 (DotU2) (↓)
REG0264	VEPGS_0621 (pppA)	T6SS00793	protein	Tpp; QS regulatory pathway	Vibrio alginolyticus strain EPJS	chromosome (FJ790777)	23577..24245 (+)	225382187	Unknown (↑); T6SS1 transcript (↑); hcp1 transcript (↓)
REG0348	SMDB11_2275 (PpkA)	T6SS00929	protein	Tpp	Serratia marcescens subsp. marcescens Db11	chromosome (HG326223)	2399154..2400599 (+)	560174118	SMDB11_2267/SMA2267 (Fha) (↓); SMDB11_2267/SMA2267 (Fha) (↑)
REG0349	SMDB11_2267 (Fha)	T6SS00929	protein	Tpp	Serratia marcescens subsp. marcescens Db11	chromosome (HG326223)	2388490..2390232 (+)	560174110	Unknown (↓)
REG0376	PA3648 (bamA)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	4085062..4087455 (-)	NP_252338.1	ClpV-GFP foci accumulate; H1-T6SS transcript (↑)
REG0377	PA0595 (lptD)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	653753..656527 (-)	NP_249286.1	ClpV-GFP foci accumulate; H1-T6SS transcript (↑)
REG0378	PA0972 (tolB)	T6SS00003	protein	Tpp	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	1056049..1057347 (+)	NP_249663.1	ClpV-GFP foci accumulate; H1-T6SS transcript (↑)