Detailed information of regulator


Summary

Regulator ID REG0348 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00929 experimental (Type i3)
Strain Serratia marcescens subsp. marcescens Db11
Replicon chromosome
Sequence Protein sequence (482 a.a.); Nucleotide sequence (1446 bp)
Description The Hcp secretion defect of the ppkA mutant could be fully complemented by expression of wild-type PpkA in trans, expression of the point mutant D165N, lacking this catalytic residue, completely failed to restore Hcp secretion; Deletion of the phosphatase gene pppA resulted in modestly reduced killing activity (shown by a 50x increase in the number of recovered target cells compared with a wild-type attacker).
Reference
External database links

Locus tag (Gene) SMDB11_2275 (PpkA)
Coordinates (Strand) 2399154..2400599 (+)
NCBI ID 560174118
GenBank HG326223
Uniprot ID UPI000324A820
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
SMDB11_2267/SMA2267 (Fha) ↓ 560174110
SMDB11_2267/SMA2267 (Fha) ↑ 560174110
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Pkinase PF00069.27 5.9e-26 23..286
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-481 MSGTGITKPNSNSLPSGYRFNEFEIQEAIGEGGFGIVYRAYDHQLERTIAIKEYMPTSLAKRNDDLSIGLRGERFSKTFQAGLNSFIQEARLLARFSHPGLLHVLRFWEENGTAYMGTQFYSGTTLKNLQAQQPEKIDEAWIRRLLPPLFSAINTIHQEGYLHRDISLDNIQIQESQLPVLLDFGSARKEIGNLSDETEIMLKPGFAPIEQYTENSDGEQGPWTDIYALGAVLHTLIVGSPPPVSVVRSIEDSYQPLAERRPAGYSPELLRTVDRALALKPEDRPQTIDEMAELLHLPIADENEIISTPAAAPENLLVAANPAAAAAAAGAVTARGTQFSRPMMAGAGVAALLVIGAVAWLSGGEDDTQAVAQNTEASPARKTLAEQPSAPQTAQPAAAEPEKVAPPPQPVALVYFKLQPGEQVSLDGKPQQVTPGANGFASLNLAPGRYQLEIRHDDRLRRQQLSIDTAGTWLVNPATAG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9909 - -


  Protein sequence of regulator: 482 a.a.    .

>REG0348 HG326223:2399154-2400599 [Serratia marcescens subsp. marcescens Db11]
MSGTGITKPNSNSLPSGYRFNEFEIQEAIGEGGFGIVYRAYDHQLERTIAIKEYMPTSLAKRNDDLSIGLRGERFSKTFQ
AGLNSFIQEARLLARFSHPGLLHVLRFWEENGTAYMGTQFYSGTTLKNLQAQQPEKIDEAWIRRLLPPLFSAINTIHQEG
YLHRDISLDNIQIQESQLPVLLDFGSARKEIGNLSDETEIMLKPGFAPIEQYTENSDGEQGPWTDIYALGAVLHTLIVGS
PPPVSVVRSIEDSYQPLAERRPAGYSPELLRTVDRALALKPEDRPQTIDEMAELLHLPIADENEIISTPAAAPENLLVAA
NPAAAAAAAGAVTARGTQFSRPMMAGAGVAALLVIGAVAWLSGGEDDTQAVAQNTEASPARKTLAEQPSAPQTAQPAAAE
PEKVAPPPQPVALVYFKLQPGEQVSLDGKPQQVTPGANGFASLNLAPGRYQLEIRHDDRLRRQQLSIDTAGTWLVNPATA
G*

  Nucleotide sequence of regulator: 1446 bp    .

>REG0348 HG326223:2399154-2400599 [Serratia marcescens subsp. marcescens Db11]
ATGTCGGGAACCGGGATAACCAAACCGAATTCGAACAGCCTGCCGAGCGGCTATCGATTCAATGAATTCGAGATCCAGGA
AGCGATCGGCGAAGGCGGCTTCGGCATCGTCTACCGCGCCTACGATCACCAACTGGAACGCACCATCGCCATCAAGGAGT
ACATGCCAACCTCGCTGGCGAAACGCAACGACGATCTCAGCATCGGGCTGCGCGGTGAACGCTTCAGCAAAACCTTCCAG
GCCGGACTGAACAGCTTTATTCAGGAGGCGCGTCTGCTGGCGCGCTTCTCGCACCCCGGCCTGCTGCACGTGTTGCGCTT
CTGGGAAGAGAACGGCACCGCCTACATGGGCACCCAGTTTTACAGCGGCACCACGCTGAAGAACCTGCAGGCGCAGCAAC
CGGAGAAGATCGACGAGGCGTGGATCCGGCGGCTGCTGCCGCCGCTGTTCAGCGCCATCAACACCATCCACCAGGAAGGC
TATCTGCACCGCGACATTTCGCTGGATAACATCCAGATCCAGGAAAGCCAACTGCCGGTGCTGCTGGACTTCGGATCGGC
GCGTAAAGAGATCGGCAACCTGTCAGACGAAACGGAGATCATGCTCAAACCGGGCTTCGCGCCGATCGAGCAGTACACCG
AGAACAGCGACGGCGAACAGGGGCCCTGGACCGATATCTACGCGCTGGGCGCCGTGCTGCACACGCTGATCGTCGGTTCG
CCGCCGCCGGTCAGCGTGGTGCGCAGCATCGAAGACAGCTACCAGCCGCTGGCCGAGCGCCGCCCCGCCGGTTACTCGCC
GGAGCTGCTGCGCACCGTCGATCGCGCGCTGGCGCTGAAGCCGGAAGACCGCCCGCAGACCATCGATGAAATGGCCGAGC
TGCTGCATCTGCCAATCGCCGATGAGAACGAAATCATCAGCACCCCTGCGGCTGCACCGGAGAACCTGCTGGTCGCCGCC
AACCCGGCTGCCGCTGCGGCCGCCGCCGGCGCCGTTACCGCGCGCGGCACCCAATTCTCGCGGCCGATGATGGCCGGCGC
CGGCGTCGCTGCGCTGCTGGTGATCGGTGCCGTCGCCTGGCTGAGCGGCGGCGAGGACGATACCCAGGCCGTGGCCCAAA
ACACTGAGGCGTCGCCGGCGCGGAAAACCCTGGCGGAACAGCCGAGCGCGCCGCAAACCGCGCAGCCTGCAGCCGCGGAA
CCTGAAAAGGTGGCACCGCCACCGCAGCCGGTGGCGTTGGTGTACTTCAAACTGCAACCGGGCGAACAGGTCAGCCTCGA
CGGCAAGCCGCAGCAGGTGACGCCGGGCGCAAACGGCTTCGCCAGCCTGAACCTGGCGCCGGGCCGCTATCAGCTGGAAA
TCCGCCACGACGATCGACTCCGCCGGCAGCAACTCAGCATCGACACCGCCGGCACCTGGCTGGTCAATCCGGCCACCGCC
GGTTAA