Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00929 experimental
T6SS type Type i3
Strain Serratia marcescens subsp. marcescens Db11
Replicon (GenBank) chromosome I (HG326223)
Location 2365124..2402670
Function anti-bacterial
Comment Killing of E. coli, P. fluorescens, Enterobacter cloacae, another strain of S. marcescens and susceptible mutants of Db10.
References
[1] Srikannathasan V et al (2013) Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallogr D Biol Crystallogr. 69(Pt 12):2468-82. [PudMed:24311588]
[2] Heymann JB et al (2013) Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. J Biol Chem. 288(35):25276-84. [PudMed:23857636]
[3] Rao VA et al (2011) The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system. Acta Crystallogr D Biol Crystallogr. 67(Pt 12):1065-72. [PudMed:22120744]
[4] Murdoch SL et al (2011) The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol. 193(21):6057-69. [PudMed:21890705]
[5] Fritsch MJ et al (2013) Proteomic identification of novel secreted anti-bacterial toxins of the Serratia marcescens Type VI secretion system. Mol Cell Proteomics. 12(10):2735-2749. [PudMed:23842002]
[6] English G et al (2012) New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 86(4):921-36. [PudMed:22957938]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number1111111121111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
SMDB11_2239 (cnu) 2360950..2361165 + 216 CDG12814.1 OriC-binding nucleoid-associated protein
SMDB11_2240 (uspC) 2361363..2361782 + 420 CDG12815.1 universal stress protein
SMDB11_2241 2361868..2362899 + 1032 CDG12816.1 putative zinc-binding alcohol dehydrogenase
SMDB11_2242 (pcp) 2362934..2363581 - 648 CDG12817.1 pyrrolidone-carboxylate peptidase
SMDB11_2243 2363679..2364611 + 933 CDG12818.1 LysR-family transcriptional regulator
T6CP012947 TssI SMDB11_2244 2365103..2367463 + 2361 CDG12819.1 type VI secretion system, effector protein VgrG-like
SMDB11_2245 2367474..2367863 + 390 CDG12820.1 hypothetical protein
SMDB11_2246 2367965..2368465 + 501 CDG12821.1 hypothetical protein
SMDB11_2247 2368637..2371189 + 2553 CDG12822.1 pentapeptide repeat-containing protein
SMDB11_2248 2371186..2372253 + 1068 CDG12823.1 pentapeptide repeat-containing protein
SMDB11_2249 2372284..2372919 + 636 CDG12824.1 hypothetical protein
SMDB11_2250 2372936..2373328 + 393 CDG12825.1 hypothetical protein
SMDB11_2251 2373377..2373877 + 501 CDG12826.1 hypothetical protein
T6CP012948 TssJ SMDB11_2252 2373907..2374437 + 531 CDG12827.1 type VI secretion system-associated protein,SciN-like
T6CP012949 TssK SMDB11_2253 2374478..2375824 + 1347 CDG12828.1 type VI secretion system-associated protein
T6CP012950 TssL SMDB11_2254 2375828..2377048 + 1221 CDG12829.1 type VI secretion system-associated protein
T6CP012951 TssM SMDB11_2255 2377048..2380683 + 3636 CDG12830.1 type VI secretion system-associated protein,IcmF-like
SMDB11_2256 2380702..2381421 + 720 CDG12831.1 type VI secretion system-associated protein
T6CP012952 TssA SMDB11_2257 2381445..2382476 + 1032 CDG12832.1 type VI secretion system-associated protein
T6CP012953 TssB SMDB11_2258 2382597..2383133 + 537 CDG12833.1 type VI secretion system-associated protein
T6CP012954 TssC SMDB11_2259 2383137..2384642 + 1506 CDG12834.1 type VI secretion system-associated protein
SMDB11_2260 2384710..2385093 + 384 CDG12835.1 hypothetical protein
SMDB11_2261 2385090..2385581 + 492 CDG12836.1 hypothetical protein
SMDB11_2262 2385581..2386036 + 456 CDG12837.1 hypothetical protein
T6CP012955 TssD SMDB11_2263 2386232..2386714 + 483 CDG12838.1 type VI secretion system, effector protein Hcp-like
SMDB11_2264 2386769..2387245 + 477 CDG12839.1 hypothetical protein
SMDB11_2265 2387230..2387604 + 375 CDG12840.1 hypothetical protein
SMDB11_2266 2387650..2388009 + 360 CDG12841.1 hypothetical protein
SMDB11_2267 2388490..2390232 + 1743 CDG12842.1 type VI secretion system-associated protein,containing FHA domain
SMDB11_2268 2390232..2391005 + 774 CDG12843.1 type VI secretion system, serine/threonine protein phosphatase, PppA-like
SMDB11_2269 2391034..2392020 + 987 CDG12844.1 hypothetical protein
SMDB11_2270 2392036..2392857 + 822 CDG12845.1 type VI secretion system-associated protein,SciE-like
T6CP012956 TssE SMDB11_2271 2392860..2393435 + 576 CDG12846.1 type VI secretion system,lysozyme-relatedprotein
T6CP012957 TssF SMDB11_2272 2393440..2395326 + 1887 CDG12847.1 type VI secretion system-associated protein
T6CP012958 TssG SMDB11_2273 2395323..2396393 + 1071 CDG12848.1 type VI secretion system-associated protein
T6CP012959 TssH SMDB11_2274 2396450..2399104 + 2655 CDG12849.1 type VI secretion system ATPase, ClpV1-like
SMDB11_2275 2399154..2400599 + 1446 CDG12850.1 type VI secretion system, serine/threonine protein kinase
T6CP012960 TssI SMDB11_2276 2400742..2402670 + 1929 CDG12851.1 type VI secretion system, effector protein VgrG-like
SMDB11_2277 2402725..2403165 + 441 CDG12852.1 hypothetical protein
SMDB11_2278 2403168..2407589 + 4422 CDG12853.1 Rhs-family protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0014 experimental SMDB11_2275 (PpKA) protein Tpp Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2399154..2400599 (+) 560174118 SMDB11_2267 (FHA) (↓)
REG0015 experimental SMDB11_2268 (PppA) protein Tpp Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2390232..2391005 (+) 560174111 SMDB11_2267 (FHA) (↑)
REG0016 experimental SMDB11_2269 (RtkS) protein Tpp Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2391034..2392020 (+) 560174112 SMDB11_2275 (PpKA) (↓)
REG0017 experimental SMDB11_2256 (TagF) protein Tpp Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2380702..2381421 (+) 560174099 Unknown (↑)
REG0348 experimental SMDB11_2275 (PpkA) protein Tpp Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2399154..2400599 (+) 560174118 SMDB11_2267/SMA2267 (Fha) (↓); SMDB11_2267/SMA2267 (Fha) (↑)
REG0349 experimental SMDB11_2267 (Fha) protein Tpp Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2388490..2390232 (+) 560174110 Unknown (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01503 experimental SMDB11_1112 (Ssp3) Tme - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 1178412..1178963 (+) 560172958
EFF01506 experimental SMDB11_4673 (Ssp6) Tme - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 5022150..5022797 (-) 560176492
EFF01514 experimental SMDB11_1083 (Tfe2) Tme - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 1147856..1148536 (+) 560172929
EFF01836 experimental SMDB11_0810 Tme IMU00354 experimental Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 838784..839398 (-) 560172663
EFF01502 experimental SMDB11_2261 (Ssp1) Tae - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2385090..2385581 (+) 560174104
EFF01510 experimental SMDB11_2264 (ssp2) Tae - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2386769..2387245 (+) 560174107
EFF01505 experimental SMDB11_4628 (Ssp5) Tse - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 4979889..4980716 (+) 560176449
EFF01499 experimental SMDB11_1610 (Rhs2) Tde IMU00320 experimental Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 1689650..1693942 (+) 560173453
EFF01515 experimental SMDB11_1610 (Rhs2) Tse - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 1693944..1694432 (+) 560173454
EFF01501 experimental SMDB11_0927 (Slp) Tle Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 971234..972886 (+) 560172775
EFF01516 experimental SMDB11_2278 (Rhs1) Tse - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2407591..2408079 (+) 560174122
EFF01500 experimental SMDB11_2278 (Rhs1) Tse IMU00319 experimental Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2403168..2407589 (+) 560174121
EFF01504 experimental SMDB11_3980 (Ssp4) Tse - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 4242848..4243843 (-) 560175802
EFF01507 experimental SMDB11_3455 (Hcp) - - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 3668310..3668792 (+) 560175282
EFF01508 experimental SMDB11_3456 (Hcp) - - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 3668840..3669322 (+) 560175283
EFF01509 experimental SMDB11_2263 (Hcp) - - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2386232..2386714 (+) 560174106
EFF01512 experimental SMDB11_2244 (VgrG) - - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2365103..2367463 (+) 560174087
EFF01513 experimental SMDB11_2276 (VgrG) - - Serratia marcescens subsp. marcescens Db11 chromosome (HG326223) 2400742..2402670 (+) 560174119