Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00929  |
| T6SS type | Type i3 |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon (GenBank) | chromosome I (HG326223) |
| Location | 2365124..2402670 |
| Function | anti-bacterial |
| Comment | Killing of E. coli, P. fluorescens, Enterobacter cloacae, another strain of S. marcescens and susceptible mutants of Db10. |
| References | [1] Heymann JB et al (2013) Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. J Biol Chem. 288(35):25276-84. [PudMed:23857636] [2] Srikannathasan V et al (2013) Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallogr D Biol Crystallogr. 69(Pt 12):2468-82. [PudMed:24311588] [3] Rao VA et al (2011) The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system. Acta Crystallogr D Biol Crystallogr. 67(Pt 12):1065-72. [PudMed:22120744] [4] Fritsch MJ et al (2013) Proteomic identification of novel secreted anti-bacterial toxins of the Serratia marcescens Type VI secretion system. Mol Cell Proteomics. 12(10):2735-2749. [PudMed:23842002] [5] English G et al (2012) New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 86(4):921-36. [PudMed:22957938] [6] Murdoch SL et al (2011) The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol. 193(21):6057-69. [PudMed:21890705] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| SMDB11_2239 (cnu) | 2360950..2361165 | + | 216 | CDG12814.1 | OriC-binding nucleoid-associated protein | ||
| SMDB11_2240 (uspC) | 2361363..2361782 | + | 420 | CDG12815.1 | universal stress protein | ||
| SMDB11_2241 | 2361868..2362899 | + | 1032 | CDG12816.1 | putative zinc-binding alcohol dehydrogenase | ||
| SMDB11_2242 (pcp) | 2362934..2363581 | - | 648 | CDG12817.1 | pyrrolidone-carboxylate peptidase | ||
| SMDB11_2243 | 2363679..2364611 | + | 933 | CDG12818.1 | LysR-family transcriptional regulator | ||
| T6CP012947 | TssI | SMDB11_2244 | 2365103..2367463 | + | 2361 | CDG12819.1 | type VI secretion system, effector protein VgrG-like |
| SMDB11_2245 | 2367474..2367863 | + | 390 | CDG12820.1 | hypothetical protein | ||
| SMDB11_2246 | 2367965..2368465 | + | 501 | CDG12821.1 | hypothetical protein | ||
| SMDB11_2247 | 2368637..2371189 | + | 2553 | CDG12822.1 | pentapeptide repeat-containing protein | ||
| SMDB11_2248 | 2371186..2372253 | + | 1068 | CDG12823.1 | pentapeptide repeat-containing protein | ||
| SMDB11_2249 | 2372284..2372919 | + | 636 | CDG12824.1 | hypothetical protein | ||
| SMDB11_2250 | 2372936..2373328 | + | 393 | CDG12825.1 | hypothetical protein | ||
| SMDB11_2251 | 2373377..2373877 | + | 501 | CDG12826.1 | hypothetical protein | ||
| T6CP012948 | TssJ | SMDB11_2252 | 2373907..2374437 | + | 531 | CDG12827.1 | type VI secretion system-associated protein,SciN-like |
| T6CP012949 | TssK | SMDB11_2253 | 2374478..2375824 | + | 1347 | CDG12828.1 | type VI secretion system-associated protein |
| T6CP012950 | TssL | SMDB11_2254 | 2375828..2377048 | + | 1221 | CDG12829.1 | type VI secretion system-associated protein |
| T6CP012951 | TssM | SMDB11_2255 | 2377048..2380683 | + | 3636 | CDG12830.1 | type VI secretion system-associated protein,IcmF-like |
| SMDB11_2256 | 2380702..2381421 | + | 720 | CDG12831.1 | type VI secretion system-associated protein | ||
| T6CP012952 | TssA | SMDB11_2257 | 2381445..2382476 | + | 1032 | CDG12832.1 | type VI secretion system-associated protein |
| T6CP012953 | TssB | SMDB11_2258 | 2382597..2383133 | + | 537 | CDG12833.1 | type VI secretion system-associated protein |
| T6CP012954 | TssC | SMDB11_2259 | 2383137..2384642 | + | 1506 | CDG12834.1 | type VI secretion system-associated protein |
| SMDB11_2260 | 2384710..2385093 | + | 384 | CDG12835.1 | hypothetical protein | ||
| SMDB11_2261 | 2385090..2385581 | + | 492 | CDG12836.1 | hypothetical protein | ||
| SMDB11_2262 | 2385581..2386036 | + | 456 | CDG12837.1 | hypothetical protein | ||
| T6CP012955 | TssD | SMDB11_2263 | 2386232..2386714 | + | 483 | CDG12838.1 | type VI secretion system, effector protein Hcp-like |
| SMDB11_2264 | 2386769..2387245 | + | 477 | CDG12839.1 | hypothetical protein | ||
| SMDB11_2265 | 2387230..2387604 | + | 375 | CDG12840.1 | hypothetical protein | ||
| SMDB11_2266 | 2387650..2388009 | + | 360 | CDG12841.1 | hypothetical protein | ||
| SMDB11_2267 | 2388490..2390232 | + | 1743 | CDG12842.1 | type VI secretion system-associated protein,containing FHA domain | ||
| SMDB11_2268 | 2390232..2391005 | + | 774 | CDG12843.1 | type VI secretion system, serine/threonine protein phosphatase, PppA-like | ||
| SMDB11_2269 | 2391034..2392020 | + | 987 | CDG12844.1 | hypothetical protein | ||
| SMDB11_2270 | 2392036..2392857 | + | 822 | CDG12845.1 | type VI secretion system-associated protein,SciE-like | ||
| T6CP012956 | TssE | SMDB11_2271 | 2392860..2393435 | + | 576 | CDG12846.1 | type VI secretion system,lysozyme-relatedprotein |
| T6CP012957 | TssF | SMDB11_2272 | 2393440..2395326 | + | 1887 | CDG12847.1 | type VI secretion system-associated protein |
| T6CP012958 | TssG | SMDB11_2273 | 2395323..2396393 | + | 1071 | CDG12848.1 | type VI secretion system-associated protein |
| T6CP012959 | TssH | SMDB11_2274 | 2396450..2399104 | + | 2655 | CDG12849.1 | type VI secretion system ATPase, ClpV1-like |
| SMDB11_2275 | 2399154..2400599 | + | 1446 | CDG12850.1 | type VI secretion system, serine/threonine protein kinase | ||
| T6CP012960 | TssI | SMDB11_2276 | 2400742..2402670 | + | 1929 | CDG12851.1 | type VI secretion system, effector protein VgrG-like |
| SMDB11_2277 | 2402725..2403165 | + | 441 | CDG12852.1 | hypothetical protein | ||
| SMDB11_2278 | 2403168..2407589 | + | 4422 | CDG12853.1 | Rhs-family protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed. Experimental investigation has been performed. Bioinformatics investigation has been performed. Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00316 |
SMDB11_0928 | EFF01501 |
Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 972883..973656 (+) | 560172776 |
| IMU00318 |
SMDB11_0929 | EFF01501 |
Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 973746..974489 (+) | 560172777 |
| IMU00319 |
SMDB11_2278A (RhsI1) | EFF01500 |
Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 2407591..2408079 (+) | 560174122 |
| IMU00320 |
SMDB11_1611 (RhsI2) | EFF01499 |
Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 1693944..1694432 (+) | 560173454 |
| IMU00354 |
SMDB11_0809 | EFF01836 |
Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 838572..838787 (-) | 560172662 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|---|---|---|---|---|---|---|---|
| ACP00028 |
SMDB11_4101 (dsbA1) | chaperone | Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 4377737..4378360 (-) | 560175924 | SMDB11_2255 (TssM); SMDB11_2251; SMDB11_2269 | SMDB11_3980 (Ssp4); SMDB11_2264 (Ssp2) |
| ACP00029 |
SMDB11_0239 (dsbA2) | chaperone | Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 265001..265642 (+) | 560172104 | SMDB11_2255 (TssM); SMDB11_2251; SMDB11_2269 | SMDB11_3980 (Ssp4); SMDB11_2264 (Ssp2) |
| ACP00030 |
SMDB11_1609 (eagR2) | adaptor | Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 1689187..1689618 (+) | 560173452 | SMDB11_2276 (VgrG2b) | SMDB11_1610 (Rhs2) |
| ACP00031 |
SMDB11_2245 (eagR1) | adaptor | Serratia marcescens subsp. marcescens Db11 | chromosome (HG326223) | 2367474..2367863 (+) | 560174088 | SMDB11_2276 (VgrG2b) | SMDB11_2278 (Rhs1) |