Detailed information of component protein


Summary

Component ID T6CP012951
Component protein type TssM
T6SS ID (Type) T6SS00929 experimental (Type i3)
Strain Serratia marcescens subsp. marcescens Db11
Replicon chromosome I
Sequence Protein sequence (1212 a.a.); Nucleotide sequence (3636 bp)
Reference
[1] Srikannathasan V, English G, Bui NK, et al. Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2468-82. doi: 10.1107/S0907444913022725. Epub 2013 Nov 19. PMID: 24311588
[2] Heymann JB, Bartho JD, Rybakova D, et al. Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. J Biol Chem. 2013 Aug 30;288(35):25276-25284. doi: 10.1074/jbc.M113.456145. Epub 2013 Jul 15. PMID: 23857636
[3] Rao VA, Shepherd SM, English G, et al. The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system. Acta Crystallogr D Biol Crystallogr. 2011 Dec;67(Pt 12):1065-72. doi: 10.1107/S0907444911046300. Epub 2011 Nov 18. PMID: 22120744
[4] Murdoch SL, Trunk K, English G, et al. The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol. 2011 Nov;193(21):6057-69. doi: 10.1128/JB.05671-11. Epub 2011 Sep 2. PMID: 21890705
[5] Fritsch MJ, Trunk K, Diniz JA, et al. Proteomic identification of novel secreted antibacterial toxins of the Serratia marcescens type VI secretion system. Mol Cell Proteomics. 2013 Oct;12(10):2735-49. doi: 10.1074/mcp.M113.030502. Epub 2013 Jul 9. PMID: 23842002
[6] English G, Trunk K, Rao VA, et al. New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 2012 Nov;86(4):921-36. doi: 10.1111/mmi.12028. Epub 2012 Sep 27. PMID: 22957938
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) SMDB11_2255
Coordinate (Strand) 2377048..2380683 (+)
NCBI ID CDG12830.1
GenBank HG326223
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.6e-95 210..484
IcmF-related PF06761.14 5.7e-86 536..840
IcmF_C PF06744.14 3.8e-22 1095..1197
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MLSTLFSIITSR
Transmembrane helix 13-35 LLWGFVGITALAFIIWTIGPLVA
Outside 36-49 IGDYRPLEPELNRQ
Transmembrane helix 50-72 IAIGVIYLIWFLCRLIPRLYSAW
Inside 73-475 FNSRLLSNLRAAEAVPAEDGKPEVKQDDQLAQRFDEAAQLLKKARFAPGQGDGKHRWMTRFSRQYLYQLPWYVIIGAPGAGKTTALVNSGLHFPLADRFGKAALRGVGGTRNCDWWFTNDAVLLDTAGRYTTQESQREEDAGEWQSFVALLKKYRTRQPINGVMVTISVADLLSDSAEARAAQASALRKRLIELHEQLGIHFPVYVLVTKTDLLNGFMAYFGSFDKAQRDQIWGFTFPYERSRQPDFNLNAAFEQQYALLQQRLDAALPDTLLMEHDARQRAESYLFPQEFAALRPLLGQYLEQVFATSSFETRFTPRGIYFTSGTQEGLPFDRVMGELNRYLQLPTANSGVQANAAWDSVGQETPIPAAKGQSFFLKETLESVVFQESGLAGSNRWWEYRNR
Transmembrane helix 476-498 ALHWAGYIALAVILAVLAMLWFT
Outside 499-1211 SYGNNKAYLAEVAAKVPGVERQGQSLTQLGNGDMFSLLPFLNSLLHLPDSQNFSLDDPPFTYRMGLFRGDQVGDAGNALYQKALKELLLPQVAQQIATTLRNDNHGDADFSYEALKAYQMLYLPKQYDGKFLRAWVMLNLQRNLPQGSTQKQLQQIEWHLSQLLDAQIQASPYAKDEQLVAQAQAALNRAPLSQRVYGRLKRLLLKQTDIKPVSLVDLAGPQTELAFSRKSGKPVTDGVPGLFTSQGYWKAFNDNIDPVTDTLRKEDVWVLNSQTPEQKSADLTKTIRQLYMQDFISAWDELLGDIQLANIGNLTQRISSARLLSGNPSPMRNLLVNVSKNVTLRDEKNDADSRSLLAKTEDKLNQNANRTLEALFTNRPANADGDVSAQPEQLVMAHFAPLLELAQSQGEGNKAIPFDSVLKQVDELYSYLTAVQGAANSGMSAPPSDIIPRLQAESGRLPVPFKQMLLSLAIGASSDTQRKEMENVKKRISFEVGSFCRQAIAGRYPLVARARQEVTPDDLARMFAPNSGLMDSFFRDNLQGKVDTTRANWRFTPGVDGKTLPGGEGILRSFQQAQRIRDAFFANGTATPSYRVTVRPVRMDNDILNLTLDIDGQLFKYSHGPQVPLVVSWPGTRNTNQVHLQLALANGTTASLVTSGPWALNRLVDMAQSSGTGGLGRQATFNLDGHRVTLEFTPNSIRNPFQLPAFSCP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9964 - -


  Protein sequence: 1212 a.a.    

>T6CP012951 HG326223:2377048-2380683 [Serratia marcescens subsp. marcescens Db11] [TssM]
MLSTLFSIITSRLLWGFVGITALAFIIWTIGPLVAIGDYRPLEPELNRQIAIGVIYLIWFLCRLIPRLYSAWFNSRLLSN
LRAAEAVPAEDGKPEVKQDDQLAQRFDEAAQLLKKARFAPGQGDGKHRWMTRFSRQYLYQLPWYVIIGAPGAGKTTALVN
SGLHFPLADRFGKAALRGVGGTRNCDWWFTNDAVLLDTAGRYTTQESQREEDAGEWQSFVALLKKYRTRQPINGVMVTIS
VADLLSDSAEARAAQASALRKRLIELHEQLGIHFPVYVLVTKTDLLNGFMAYFGSFDKAQRDQIWGFTFPYERSRQPDFN
LNAAFEQQYALLQQRLDAALPDTLLMEHDARQRAESYLFPQEFAALRPLLGQYLEQVFATSSFETRFTPRGIYFTSGTQE
GLPFDRVMGELNRYLQLPTANSGVQANAAWDSVGQETPIPAAKGQSFFLKETLESVVFQESGLAGSNRWWEYRNRALHWA
GYIALAVILAVLAMLWFTSYGNNKAYLAEVAAKVPGVERQGQSLTQLGNGDMFSLLPFLNSLLHLPDSQNFSLDDPPFTY
RMGLFRGDQVGDAGNALYQKALKELLLPQVAQQIATTLRNDNHGDADFSYEALKAYQMLYLPKQYDGKFLRAWVMLNLQR
NLPQGSTQKQLQQIEWHLSQLLDAQIQASPYAKDEQLVAQAQAALNRAPLSQRVYGRLKRLLLKQTDIKPVSLVDLAGPQ
TELAFSRKSGKPVTDGVPGLFTSQGYWKAFNDNIDPVTDTLRKEDVWVLNSQTPEQKSADLTKTIRQLYMQDFISAWDEL
LGDIQLANIGNLTQRISSARLLSGNPSPMRNLLVNVSKNVTLRDEKNDADSRSLLAKTEDKLNQNANRTLEALFTNRPAN
ADGDVSAQPEQLVMAHFAPLLELAQSQGEGNKAIPFDSVLKQVDELYSYLTAVQGAANSGMSAPPSDIIPRLQAESGRLP
VPFKQMLLSLAIGASSDTQRKEMENVKKRISFEVGSFCRQAIAGRYPLVARARQEVTPDDLARMFAPNSGLMDSFFRDNL
QGKVDTTRANWRFTPGVDGKTLPGGEGILRSFQQAQRIRDAFFANGTATPSYRVTVRPVRMDNDILNLTLDIDGQLFKYS
HGPQVPLVVSWPGTRNTNQVHLQLALANGTTASLVTSGPWALNRLVDMAQSSGTGGLGRQATFNLDGHRVTLEFTPNSIR
NPFQLPAFSCP*

  Nucleotide sequence: 3636 bp    

>T6CP012951 HG326223:2377048-2380683 [Serratia marcescens subsp. marcescens Db11] [TssM]
ATGCTCAGTACGTTATTCTCCATCATCACCAGCCGCCTGCTGTGGGGCTTTGTCGGCATCACCGCGCTGGCGTTCATCAT
CTGGACGATCGGCCCGCTGGTGGCCATCGGCGACTATCGCCCGCTGGAGCCGGAACTCAACCGCCAGATCGCCATCGGCG
TGATTTACTTGATCTGGTTCCTGTGCCGCCTGATCCCGCGCCTCTACAGTGCCTGGTTCAACAGCCGCCTGCTGAGCAAT
CTGCGCGCCGCCGAAGCGGTGCCGGCGGAAGACGGCAAGCCCGAGGTCAAACAAGACGATCAGTTGGCGCAGCGTTTCGA
CGAAGCGGCGCAGCTGCTGAAAAAGGCGCGCTTCGCACCGGGCCAGGGCGACGGTAAACATCGCTGGATGACCCGCTTCA
GCCGTCAATATCTGTACCAGTTGCCGTGGTATGTGATCATCGGCGCACCCGGCGCCGGCAAAACCACCGCGCTGGTCAAC
TCCGGCCTGCACTTCCCACTGGCGGATCGCTTCGGCAAAGCCGCGCTGCGCGGCGTGGGCGGCACCCGCAACTGCGACTG
GTGGTTTACCAACGACGCCGTGCTGCTCGACACCGCCGGCCGCTACACCACCCAGGAAAGCCAGCGCGAAGAAGACGCCG
GTGAATGGCAAAGCTTCGTCGCGCTGCTGAAAAAATACCGCACCCGCCAGCCGATCAACGGCGTGATGGTGACCATCAGC
GTCGCCGATCTGCTGAGCGACAGCGCCGAAGCACGCGCCGCCCAGGCCAGCGCACTGCGCAAACGCCTGATTGAACTGCA
CGAACAGTTGGGCATTCACTTCCCGGTCTATGTGCTGGTGACCAAAACCGACCTGCTCAACGGCTTTATGGCCTACTTCG
GCAGCTTCGACAAAGCGCAGCGCGACCAGATCTGGGGATTCACCTTCCCTTACGAGCGCTCACGGCAGCCGGACTTCAAC
CTGAACGCCGCCTTCGAACAACAATACGCCCTGCTGCAACAACGGCTGGATGCGGCGCTGCCGGATACGCTGCTGATGGA
GCACGACGCGCGCCAACGCGCCGAAAGCTACCTGTTCCCGCAGGAGTTCGCCGCGCTGCGCCCGCTGCTCGGCCAATATC
TGGAACAGGTGTTCGCCACCTCCAGTTTTGAAACCCGCTTTACCCCGCGCGGCATCTATTTCACCAGCGGCACGCAGGAA
GGTCTGCCGTTTGACCGGGTGATGGGCGAACTGAACCGCTACCTGCAGCTGCCGACGGCCAACTCAGGGGTGCAGGCCAA
CGCGGCCTGGGACAGCGTGGGGCAGGAAACGCCGATCCCGGCAGCCAAAGGCCAGAGCTTCTTCCTGAAAGAGACGCTGG
AATCGGTGGTCTTCCAGGAGTCCGGGCTGGCGGGCAGCAACCGCTGGTGGGAATACCGCAACCGCGCCCTGCACTGGGCA
GGGTACATCGCGCTGGCGGTGATCCTGGCGGTGCTGGCGATGTTGTGGTTCACCAGCTACGGCAACAACAAGGCCTATCT
GGCGGAAGTGGCCGCCAAGGTGCCCGGCGTCGAGCGACAGGGGCAAAGCCTGACCCAGTTGGGCAACGGCGACATGTTCT
CGCTGCTGCCGTTCCTCAACAGCCTGCTGCATCTGCCGGACAGCCAGAACTTTTCGCTGGACGATCCGCCGTTCACCTAC
CGCATGGGGCTGTTCCGCGGCGATCAGGTGGGCGACGCCGGCAATGCGTTGTACCAAAAAGCGCTGAAAGAGCTGCTGCT
GCCGCAGGTGGCGCAACAGATCGCCACCACGCTGCGCAACGACAACCACGGCGACGCGGACTTCAGCTACGAAGCGCTGA
AGGCCTATCAGATGCTGTATCTGCCAAAGCAGTACGACGGTAAATTCCTGCGCGCCTGGGTGATGCTCAACCTGCAACGC
AACCTGCCGCAGGGCAGCACGCAGAAACAGCTGCAGCAGATCGAATGGCACCTCAGCCAACTGCTGGATGCGCAGATCCA
GGCCTCGCCTTACGCCAAAGACGAACAGCTGGTGGCGCAGGCGCAGGCCGCACTCAATCGGGCGCCGCTGTCGCAGCGCG
TCTATGGCCGCCTCAAGCGCCTGCTGCTGAAACAGACCGACATCAAGCCGGTCAGCCTGGTCGATTTGGCCGGCCCGCAG
ACCGAACTGGCGTTCAGCCGCAAAAGCGGCAAACCGGTCACCGACGGCGTACCGGGCCTGTTCACCTCACAAGGGTACTG
GAAAGCGTTCAACGACAACATCGACCCGGTGACCGACACCTTGCGCAAGGAAGACGTCTGGGTGTTGAACAGCCAGACGC
CGGAGCAGAAAAGCGCCGATCTGACCAAGACCATCCGCCAGCTGTACATGCAAGATTTCATCAGCGCCTGGGATGAGCTG
CTGGGGGATATCCAACTGGCCAACATCGGCAACCTGACCCAGCGCATCAGCAGCGCGCGCCTGCTGTCCGGCAACCCGTC
GCCAATGCGCAATCTGCTGGTCAACGTCAGCAAAAACGTCACCCTGCGCGACGAAAAAAACGACGCCGATTCGCGCTCGC
TGCTGGCAAAAACCGAAGATAAGCTGAACCAAAATGCCAACCGCACGCTGGAAGCGCTGTTCACCAACCGGCCGGCCAAC
GCCGACGGTGACGTGTCGGCCCAGCCGGAACAGCTGGTGATGGCGCACTTCGCGCCGCTGCTGGAGCTGGCGCAAAGCCA
GGGCGAAGGCAATAAGGCGATCCCTTTCGACAGCGTGCTGAAACAGGTCGATGAACTGTACAGTTACCTGACGGCGGTGC
AGGGCGCGGCCAACAGCGGCATGTCGGCGCCGCCGAGCGACATTATCCCGCGTCTGCAGGCCGAATCCGGCCGCTTGCCG
GTGCCGTTCAAACAGATGCTGCTGTCGCTGGCGATCGGCGCCAGCAGCGATACCCAGCGCAAAGAGATGGAGAACGTGAA
AAAGCGCATCAGCTTCGAGGTCGGTAGCTTCTGCCGCCAGGCGATCGCCGGCCGTTATCCGCTGGTGGCCCGGGCGCGGC
AGGAAGTGACGCCGGACGATCTGGCGCGCATGTTCGCGCCGAACAGCGGGCTGATGGACAGCTTCTTCCGCGACAATCTG
CAGGGCAAGGTCGATACCACCCGCGCCAACTGGCGTTTCACGCCCGGCGTGGACGGCAAGACGCTGCCCGGCGGCGAAGG
CATCCTGCGGTCGTTCCAGCAGGCGCAGCGCATCCGCGACGCCTTTTTCGCCAACGGCACGGCGACGCCCTCCTACCGCG
TGACCGTGCGGCCGGTGCGGATGGATAACGACATCCTCAACCTGACGCTGGATATCGACGGGCAGCTGTTCAAATACAGC
CACGGCCCACAGGTACCGCTGGTGGTCAGTTGGCCGGGCACCCGCAATACCAACCAGGTGCATCTGCAGCTGGCGCTGGC
CAACGGCACCACCGCCAGCCTGGTGACCAGCGGACCATGGGCGCTGAATCGCCTGGTCGACATGGCGCAATCGTCGGGCA
CCGGCGGCCTCGGCCGCCAGGCGACGTTCAATCTCGACGGCCACCGCGTCACGCTGGAGTTCACGCCCAACAGCATCCGC
AATCCGTTCCAACTGCCGGCCTTCTCGTGCCCGTAG