Detailed information of effector
Summary
| Effector ID | EFF01514  |
| Effector type | Tme |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00929 (Type i3) |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon | chromosome |
| Sequence | Protein sequence (227 a.a.); Nucleotide sequence (681 bp) |
| Description | Tfe2 intoxication leads to extensive Tfe2 intoxication leads to extensive nutrient imbalance, interference with plasma membrane transporters and induction of autophagy |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | SMDB11_1083 (Tfe2) |
| Coordinates (Strand) | 1147856..1148536 (+) |
| NCBI ID | 560172929 |
| GenBank | HG326223 |
| Uniprot ID | UPI0003C97954 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-226 | MPLEVDGIIRGDRGSEPSRWQHASTKPLITLTWHHTIPWNCLRNVWNGLVAGQHWNALDEFMNLIGVPNRAEVITQIKNKNLQDRDGLHTLVTWQGWNIVEGPGNEYRAQGDDPGENFDGWSGKGMSTNQQATLQQVNVLYQVMAPLGSRALDAARQAPNISAEEASVLQRTIKQTRPTLRGKEPIRWQEDMWHQVQPGKEAKHFARWDTKPVWRKRLHSDLAQAG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9892 | - | - |
Protein sequence: 227 a.a. .
>EFF01514 HG326223:1147856-1148536 Type VI Membrane-disrupting Effector (Tme) [Serratia marcescens subsp. marcescens Db11]
MPLEVDGIIRGDRGSEPSRWQHASTKPLITLTWHHTIPWNCLRNVWNGLVAGQHWNALDEFMNLIGVPNRAEVITQIKNK
NLQDRDGLHTLVTWQGWNIVEGPGNEYRAQGDDPGENFDGWSGKGMSTNQQATLQQVNVLYQVMAPLGSRALDAARQAPN
ISAEEASVLQRTIKQTRPTLRGKEPIRWQEDMWHQVQPGKEAKHFARWDTKPVWRKRLHSDLAQAG*
MPLEVDGIIRGDRGSEPSRWQHASTKPLITLTWHHTIPWNCLRNVWNGLVAGQHWNALDEFMNLIGVPNRAEVITQIKNK
NLQDRDGLHTLVTWQGWNIVEGPGNEYRAQGDDPGENFDGWSGKGMSTNQQATLQQVNVLYQVMAPLGSRALDAARQAPN
ISAEEASVLQRTIKQTRPTLRGKEPIRWQEDMWHQVQPGKEAKHFARWDTKPVWRKRLHSDLAQAG*
Nucleotide sequence: 681 bp .
>EFF01514 HG326223:1147856-1148536 Type VI Membrane-disrupting Effector (Tme) [Serratia marcescens subsp. marcescens Db11]
ATGCCATTAGAAGTCGATGGGATCATTAGAGGCGATCGGGGCAGTGAACCGAGCCGTTGGCAACATGCGTCAACCAAACC
GCTTATCACGCTGACCTGGCATCACACCATTCCTTGGAACTGTTTGCGTAATGTCTGGAATGGTCTGGTGGCGGGGCAGC
ATTGGAACGCGCTGGACGAGTTTATGAACCTGATCGGTGTGCCAAACCGTGCCGAGGTGATCACACAGATCAAGAATAAA
AACCTGCAGGATCGCGATGGATTACACACTCTGGTGACCTGGCAAGGATGGAACATCGTCGAGGGGCCTGGTAATGAGTA
TCGTGCGCAGGGGGACGATCCCGGTGAAAACTTCGACGGTTGGTCGGGTAAGGGGATGAGCACTAATCAGCAAGCGACGC
TGCAGCAGGTCAACGTGTTGTATCAGGTGATGGCGCCGCTGGGAAGCCGTGCGCTGGACGCCGCCAGACAGGCGCCGAAC
ATTTCTGCGGAAGAAGCGAGCGTCTTGCAACGGACGATTAAACAGACCCGTCCAACGTTGCGAGGAAAGGAACCGATCCG
GTGGCAAGAAGACATGTGGCATCAGGTGCAGCCGGGTAAAGAGGCGAAACACTTCGCCCGTTGGGACACCAAACCGGTGT
GGCGCAAGCGCCTGCACAGCGATCTGGCTCAGGCTGGATAA
ATGCCATTAGAAGTCGATGGGATCATTAGAGGCGATCGGGGCAGTGAACCGAGCCGTTGGCAACATGCGTCAACCAAACC
GCTTATCACGCTGACCTGGCATCACACCATTCCTTGGAACTGTTTGCGTAATGTCTGGAATGGTCTGGTGGCGGGGCAGC
ATTGGAACGCGCTGGACGAGTTTATGAACCTGATCGGTGTGCCAAACCGTGCCGAGGTGATCACACAGATCAAGAATAAA
AACCTGCAGGATCGCGATGGATTACACACTCTGGTGACCTGGCAAGGATGGAACATCGTCGAGGGGCCTGGTAATGAGTA
TCGTGCGCAGGGGGACGATCCCGGTGAAAACTTCGACGGTTGGTCGGGTAAGGGGATGAGCACTAATCAGCAAGCGACGC
TGCAGCAGGTCAACGTGTTGTATCAGGTGATGGCGCCGCTGGGAAGCCGTGCGCTGGACGCCGCCAGACAGGCGCCGAAC
ATTTCTGCGGAAGAAGCGAGCGTCTTGCAACGGACGATTAAACAGACCCGTCCAACGTTGCGAGGAAAGGAACCGATCCG
GTGGCAAGAAGACATGTGGCATCAGGTGCAGCCGGGTAAAGAGGCGAAACACTTCGCCCGTTGGGACACCAAACCGGTGT
GGCGCAAGCGCCTGCACAGCGATCTGGCTCAGGCTGGATAA