Detailed information of effector
Summary
| Effector ID | EFF01506  |
| Effector type | Tme |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00929 (Type i3) |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon | chromosome |
| Sequence | Protein sequence (216 a.a.); Nucleotide sequence (648 bp) |
| Description | SMA4673 (Ssp6)Ssp6 forms ion-selective membrane pores in vitro and Ssp6 intoxication impairs the integrity of the outer membrane. |
| Reference | [1] Mariano G, Trunk K, Williams DJ, et al. A family of Type VI secretion system effector proteins that form ion-selective pores. Nat Commun. 2019 Dec 2;10(1):5484. doi: 10.1038/s41467-019-13439-0. PMID: 31792213 [2] Mariano G, Trunk K, Williams DJ, et al. A family of Type VI secretion system effector proteins that form ion-selective pores. Nat Commun. 2019 Dec 2;10(1):5484. doi: 10.1038/s41467-019-13439-0. PMID: 31792213 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | SMDB11_4673 (Ssp6) |
| Coordinates (Strand) | 5022150..5022797 (-) |
| NCBI ID | 560176492 |
| GenBank | HG326223 |
| Uniprot ID | UPI0003C9BF24 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-215 | MAKGAKEIAQEMANAVNSKSNFFGGFIEGAISFPVDIGYLAYDFINTDNRSINRYDTERMLRLIKAGLANQHSLTKIVKLVVDEYLKKVDVDKVKRWVEKGSGKIAGRFVSNQVLMVNLGAVLSERVVIRLATGYALTSLLTLGAMNSRAIHTSRQLRQRNPEIYDNLRRAGNLDLLYFLVEPKTKPFEQAIEIWRKNRGEFDRITELFFEKVSK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9883 | - | - |
Protein sequence: 216 a.a. .
>EFF01506 HG326223:c5022797-5022150 Type VI Membrane-disrupting Effector (Tme) [Serratia marcescens subsp. marcescens Db11]
MAKGAKEIAQEMANAVNSKSNFFGGFIEGAISFPVDIGYLAYDFINTDNRSINRYDTERMLRLIKAGLANQHSLTKIVKL
VVDEYLKKVDVDKVKRWVEKGSGKIAGRFVSNQVLMVNLGAVLSERVVIRLATGYALTSLLTLGAMNSRAIHTSRQLRQR
NPEIYDNLRRAGNLDLLYFLVEPKTKPFEQAIEIWRKNRGEFDRITELFFEKVSK*
MAKGAKEIAQEMANAVNSKSNFFGGFIEGAISFPVDIGYLAYDFINTDNRSINRYDTERMLRLIKAGLANQHSLTKIVKL
VVDEYLKKVDVDKVKRWVEKGSGKIAGRFVSNQVLMVNLGAVLSERVVIRLATGYALTSLLTLGAMNSRAIHTSRQLRQR
NPEIYDNLRRAGNLDLLYFLVEPKTKPFEQAIEIWRKNRGEFDRITELFFEKVSK*
Nucleotide sequence: 648 bp .
>EFF01506 HG326223:c5022797-5022150 Type VI Membrane-disrupting Effector (Tme) [Serratia marcescens subsp. marcescens Db11]
ATGGCAAAAGGTGCGAAGGAAATCGCCCAAGAGATGGCGAACGCTGTAAATTCAAAAAGTAATTTCTTTGGCGGTTTTAT
CGAAGGGGCTATCTCCTTTCCCGTCGATATAGGATACCTGGCATATGATTTTATCAATACAGATAACCGCTCTATAAATC
GATATGACACAGAAAGAATGCTTCGCCTCATTAAAGCTGGACTTGCTAACCAACACTCACTGACCAAAATCGTTAAACTT
GTCGTTGACGAATATCTGAAAAAAGTCGATGTAGATAAAGTCAAAAGATGGGTTGAAAAAGGATCCGGGAAAATTGCAGG
TAGGTTTGTGAGCAATCAGGTTCTGATGGTTAACTTAGGGGCGGTGCTTTCCGAACGGGTGGTTATTCGCCTCGCAACAG
GTTATGCCCTAACGTCACTGCTGACCCTCGGAGCTATGAACTCAAGAGCAATCCATACCTCACGCCAGTTACGACAGCGA
AACCCTGAAATTTACGATAACTTAAGGCGTGCGGGAAATTTGGATCTCTTATATTTTCTGGTAGAACCCAAAACAAAGCC
ATTTGAACAAGCGATAGAGATTTGGCGAAAAAACAGAGGCGAGTTTGACCGCATTACCGAGCTATTTTTTGAAAAGGTGT
CGAAATGA
ATGGCAAAAGGTGCGAAGGAAATCGCCCAAGAGATGGCGAACGCTGTAAATTCAAAAAGTAATTTCTTTGGCGGTTTTAT
CGAAGGGGCTATCTCCTTTCCCGTCGATATAGGATACCTGGCATATGATTTTATCAATACAGATAACCGCTCTATAAATC
GATATGACACAGAAAGAATGCTTCGCCTCATTAAAGCTGGACTTGCTAACCAACACTCACTGACCAAAATCGTTAAACTT
GTCGTTGACGAATATCTGAAAAAAGTCGATGTAGATAAAGTCAAAAGATGGGTTGAAAAAGGATCCGGGAAAATTGCAGG
TAGGTTTGTGAGCAATCAGGTTCTGATGGTTAACTTAGGGGCGGTGCTTTCCGAACGGGTGGTTATTCGCCTCGCAACAG
GTTATGCCCTAACGTCACTGCTGACCCTCGGAGCTATGAACTCAAGAGCAATCCATACCTCACGCCAGTTACGACAGCGA
AACCCTGAAATTTACGATAACTTAAGGCGTGCGGGAAATTTGGATCTCTTATATTTTCTGGTAGAACCCAAAACAAAGCC
ATTTGAACAAGCGATAGAGATTTGGCGAAAAAACAGAGGCGAGTTTGACCGCATTACCGAGCTATTTTTTGAAAAGGTGT
CGAAATGA