SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01502
Effector type	Tae
Cognate immunity protein	-
Related T6SS (Type)	T6SS00929 (Type i3)
Strain	Serratia marcescens subsp. marcescens Db11
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Description	SMA2261 (Ssp1)Periplasmic Ssp1 was toxic and its effect alleviated by co-production of Rap1ab. However Ssp1 toxicity was only observed at higher expression levels and on minimal media.Ssp1 and Ssp2 are distinctive peptidoglycan dl-endopeptidases in terms of specificity, with the former being more promiscuous regarding the chemical structure that it is able to recognize and then cleave
Reference	[1] English G, Trunk K, Rao VA, et al. New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 2012 Nov;86(4):921-36. doi: 10.1111/mmi.12028. Epub 2012 Sep 27. PMID: 22957938 [2] Srikannathasan V, English G, Bui NK, et al. Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2468-82. doi: 10.1107/S0907444913022725. Epub 2013 Nov 19. PMID: 24311588

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	SMDB11_2261 (Ssp1)
Coordinates (Strand)	2385090..2385581 (+)
NCBI ID	560174104
GenBank	HG326223
Uniprot ID	UPI000325B5E7
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Tae4	PF14113.8	1.3e-16	46..160

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-163	MKPLYRQLKSSHYSSDYSSPGYLAAEAVYAEIGYELDTLLKQNPGYANTCAVRMSLALLKTGISFKGRLPIKKGAYKGKTIEPGAKLLADQLHRSSSFGKAKIFFNAPDAEKGIGNKKGVVFFNKITNYDGGHIDLIEPENSLLTCHSHCYFNCKEVWFWELS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9766	-	-

Protein sequence: 164 a.a. .

                                    >EFF01502 HG326223:2385090-2385581 Type VI Amidase Effector (Tae) [Serratia marcescens subsp. marcescens Db11]
MKPLYRQLKSSHYSSDYSSPGYLAAEAVYAEIGYELDTLLKQNPGYANTCAVRMSLALLKTGISFKGRLPIKKGAYKGKT
IEPGAKLLADQLHRSSSFGKAKIFFNAPDAEKGIGNKKGVVFFNKITNYDGGHIDLIEPENSLLTCHSHCYFNCKEVWFW
ELS*

                                

Nucleotide sequence: 492 bp .

                                    >EFF01502 HG326223:2385090-2385581 Type VI Amidase Effector (Tae) [Serratia marcescens subsp. marcescens Db11]
ATGAAACCTTTATATCGTCAGCTAAAAAGCAGCCATTACTCCTCTGATTACTCTAGCCCGGGATATTTGGCAGCAGAAGC
CGTTTACGCTGAGATTGGCTATGAGCTTGATACGCTTCTAAAGCAAAATCCTGGTTATGCCAATACCTGTGCCGTCCGCA
TGAGCCTGGCGCTGTTGAAAACCGGTATCAGCTTCAAAGGCCGCCTGCCAATCAAGAAAGGCGCCTATAAGGGTAAAACG
ATTGAACCGGGAGCCAAATTGCTGGCCGATCAGTTGCATCGCTCCAGTAGTTTTGGCAAAGCCAAAATTTTCTTTAATGC
TCCAGATGCAGAGAAAGGTATTGGCAATAAAAAAGGAGTCGTGTTCTTCAATAAGATCACCAACTATGATGGCGGACACA
TTGATCTTATCGAACCAGAAAACTCGCTATTAACCTGCCATTCCCATTGCTATTTTAACTGCAAAGAGGTCTGGTTTTGG
GAGTTGAGCTAG

                                