SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00318
Cognate effector	EFF01501
Related T6SS (Type)	T6SS00929 (Type i3)
Strain	Serratia marcescens subsp. marcescens Db11
Replicon	chromosome
Sequence	Protein sequence (248 a.a.); Nucleotide sequence (744 bp)
Description	Immune toxin SMDB11_0927 (Slp,Secreted lipase-like protein) with SMDB11_0928
Reference	[1] Cianfanelli FR, Alcoforado Diniz J, Guo M, et al. VgrG and PAAR Proteins Define Distinct Versions of a Functional Type VI Secretion System. PLoS Pathog. 2016 Jun 28;12(6):e1005735. doi: 10.1371/journal.ppat.1005735. eCollection 2016 Jun. PMID: 27352036

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	SMDB11_0929
Coordinates (Strand)	973746..974489 (+)
NCBI ID	560172777
GenBank	HG326223
Uniprot ID	UPI0003C9E66A
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Ank_2	PF12796.9	4.1e-05	147..209

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-247	MRHSALIIIMLLSMLMMQGCEQGRDLQPQDYFEGKQLDIANVIYEGDRQKLDKVLPTVSKETLNRPAKAEMTLLFWAINNAIFDKNTPERLKIITDLVKAGADPLQPRAEGGSSPAEFVMKANKGVWIQAMLEGGLSPNARDKVHNQPIIFESFDAANTETLKILIAYKADVNIKGAMNRTPLINALYNSCPEHIEVLLAHGANPLAKDDFNDSFLSLISAEIDKGDKSNAYIKKLIKIKEKIKQVN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9983	pos: 19-20. MQG-CE.	MRHSALIIIMLLSMLMMQG

Protein sequence: 248 a.a. .

                                    >IMU00318 HG326223:973746-974489 [Serratia marcescens subsp. marcescens Db11]
MRHSALIIIMLLSMLMMQGCEQGRDLQPQDYFEGKQLDIANVIYEGDRQKLDKVLPTVSKETLNRPAKAEMTLLFWAINN
AIFDKNTPERLKIITDLVKAGADPLQPRAEGGSSPAEFVMKANKGVWIQAMLEGGLSPNARDKVHNQPIIFESFDAANTE
TLKILIAYKADVNIKGAMNRTPLINALYNSCPEHIEVLLAHGANPLAKDDFNDSFLSLISAEIDKGDKSNAYIKKLIKIK
EKIKQVN*

                                

Nucleotide sequence: 744 bp .

                                    >IMU00318 HG326223:973746-974489 [Serratia marcescens subsp. marcescens Db11]
ATGAGGCACAGCGCTCTTATCATTATCATGTTGCTGTCAATGCTCATGATGCAGGGATGCGAACAAGGTAGGGATCTACA
ACCACAGGATTACTTTGAAGGGAAGCAGCTGGACATCGCCAACGTCATCTACGAGGGTGACAGGCAAAAGCTGGATAAGG
TTTTACCGACGGTAAGCAAAGAAACGTTAAATCGGCCGGCCAAGGCGGAGATGACGTTATTATTCTGGGCGATAAATAAC
GCTATCTTTGACAAGAACACGCCAGAGCGGCTCAAGATCATTACGGATCTGGTCAAGGCGGGAGCAGACCCACTGCAGCC
AAGAGCTGAGGGGGGGAGTAGCCCCGCTGAATTTGTTATGAAGGCGAATAAAGGCGTATGGATACAAGCTATGTTAGAGG
GCGGGCTCTCTCCAAATGCCAGAGATAAGGTTCATAATCAACCCATTATATTTGAAAGTTTTGATGCGGCGAACACCGAA
ACATTAAAGATATTAATTGCATACAAAGCTGATGTTAATATCAAAGGGGCTATGAACAGAACGCCATTAATTAATGCGCT
GTATAACAGTTGCCCAGAACACATTGAGGTTCTTTTGGCTCATGGCGCTAACCCCTTAGCCAAAGATGATTTTAATGACA
GCTTCCTCTCTCTGATTTCTGCGGAGATAGATAAAGGGGATAAGAGCAATGCGTATATAAAAAAGCTAATTAAAATTAAA
GAAAAAATAAAACAGGTGAATTAG

                                