SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00354
Cognate effector	EFF01836
Related T6SS (Type)	T6SS00929 (Type i3)
Strain	Serratia marcescens subsp. marcescens Db11
Replicon	chromosome
Sequence	Protein sequence (72 a.a.); Nucleotide sequence (216 bp)
Description	SMDB11_0809, a homologue of Sip6. SMDB11_0810 induces detectable depolarisation in a small fraction of the cells. This effect was recovered back to the level of the empty vector control by the co-expression of the putative immunity protein, SMDB11_0809.
Reference	[1] Mariano G, Trunk K, Williams DJ, et al. A family of Type VI secretion system effector proteins that form ion-selective pores. Nat Commun. 2019 Dec 2;10(1):5484. doi: 10.1038/s41467-019-13439-0. PMID: 31792213

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	SMDB11_0809
Coordinates (Strand)	838572..838787 (-)
NCBI ID	560172662
GenBank	HG326223
Uniprot ID	UPI0003C97DC1
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-11	MKNFMLAALSR
Transmembrane helix	12-34	IIQGIGCGVVALSLLAIVWFMFY
Outside	35-38	SDDS
Transmembrane helix	39-58	FKYLWVATSIAGIFLGYFIF
Inside	59-71	RFAVKKIHDGSPD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9014	-	-

Protein sequence: 72 a.a. .

                                    >IMU00354 HG326223:c838787-838572 [Serratia marcescens subsp. marcescens Db11]
MKNFMLAALSRIIQGIGCGVVALSLLAIVWFMFYSDDSFKYLWVATSIAGIFLGYFIFRFAVKKIHDGSPD*

Nucleotide sequence: 216 bp .

                                    >IMU00354 HG326223:c838787-838572 [Serratia marcescens subsp. marcescens Db11]
ATGAAGAACTTTATGCTGGCGGCTTTATCCAGAATTATTCAGGGCATTGGGTGCGGCGTTGTGGCCCTCTCGCTGTTAGC
CATTGTCTGGTTCATGTTTTATTCCGACGACAGTTTTAAGTATCTGTGGGTTGCGACCTCCATAGCTGGAATTTTCTTAG
GGTATTTCATTTTCAGGTTTGCGGTTAAAAAAATACACGACGGAAGCCCTGATTAA

                                