Detailed information of effector
Summary
| Effector ID | EFF01836  |
| Effector type | Tme |
| Cognate immunity protein | IMU00354 |
| Related T6SS (Type) | T6SS00929 (Type i3) |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon | chromosome |
| Sequence | Protein sequence (205 a.a.); Nucleotide sequence (615 bp) |
| Description | SMDB11_0810, a second homologue of Ssp6.SMDB11_0810 induces detectable depolarisation in a small fraction of the cells. This effect was recovered back to the level of the empty vector control by the co-expression of the putative immunity protein, SMDB11_0809. Expression of SMDB11_0810 also led to an increase in NPN uptake. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | SMDB11_0810 |
| Coordinates (Strand) | 838784..839398 (-) |
| NCBI ID | 560172663 |
| GenBank | HG326223 |
| Uniprot ID | UPI0003C9DF06 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-204 | MADAVNIDSNLVRGFAQGFISVPVDFYYLGYDYIDTENRWLNSQDKERFIRLIKSGLANKASIEKVAHIFIENFASKVKFEDVKDMTIRTSGKLIGKFAFNQLATANMGYIFSKQIIPRLVSGVAVGSILTAGASISRSVYTSRDLRARNSRMYDQLRRLGNLDLLYFLLENKVRPFEDAVSVWEKDRKKFSQICQYFFAKVNV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9962 | - | - |
Protein sequence: 205 a.a. .
>EFF01836 HG326223:c839398-838784 Type VI Membrane-disrupting Effector (Tme) [Serratia marcescens subsp. marcescens Db11]
MADAVNIDSNLVRGFAQGFISVPVDFYYLGYDYIDTENRWLNSQDKERFIRLIKSGLANKASIEKVAHIFIENFASKVKF
EDVKDMTIRTSGKLIGKFAFNQLATANMGYIFSKQIIPRLVSGVAVGSILTAGASISRSVYTSRDLRARNSRMYDQLRRL
GNLDLLYFLLENKVRPFEDAVSVWEKDRKKFSQICQYFFAKVNV*
MADAVNIDSNLVRGFAQGFISVPVDFYYLGYDYIDTENRWLNSQDKERFIRLIKSGLANKASIEKVAHIFIENFASKVKF
EDVKDMTIRTSGKLIGKFAFNQLATANMGYIFSKQIIPRLVSGVAVGSILTAGASISRSVYTSRDLRARNSRMYDQLRRL
GNLDLLYFLLENKVRPFEDAVSVWEKDRKKFSQICQYFFAKVNV*
Nucleotide sequence: 615 bp .
>EFF01836 HG326223:c839398-838784 Type VI Membrane-disrupting Effector (Tme) [Serratia marcescens subsp. marcescens Db11]
ATGGCGGATGCCGTAAACATTGACAGCAATTTAGTGCGCGGGTTTGCACAAGGTTTTATCAGCGTACCGGTCGATTTCTA
CTACCTGGGCTACGACTATATTGATACTGAAAACAGATGGTTAAACTCGCAAGATAAAGAGCGATTTATCAGACTCATCA
AGTCAGGCCTGGCCAATAAAGCAAGCATCGAGAAAGTGGCTCATATCTTCATCGAAAACTTCGCCAGTAAGGTCAAGTTT
GAAGATGTTAAGGACATGACCATAAGAACATCAGGCAAACTCATCGGCAAATTCGCCTTTAATCAGTTAGCCACCGCAAA
CATGGGATATATATTCTCTAAACAAATCATCCCACGGCTTGTCTCCGGTGTTGCCGTTGGCTCAATTCTCACCGCTGGGG
CAAGCATATCGCGCTCTGTCTATACATCACGCGACTTGAGGGCAAGAAACTCACGGATGTATGATCAACTCAGACGCCTG
GGAAACCTCGATCTGCTGTACTTTCTGTTAGAAAACAAAGTCCGACCATTCGAGGATGCCGTCTCGGTTTGGGAAAAAGA
TCGAAAGAAATTCAGCCAAATTTGTCAGTACTTTTTTGCAAAGGTTAACGTATGA
ATGGCGGATGCCGTAAACATTGACAGCAATTTAGTGCGCGGGTTTGCACAAGGTTTTATCAGCGTACCGGTCGATTTCTA
CTACCTGGGCTACGACTATATTGATACTGAAAACAGATGGTTAAACTCGCAAGATAAAGAGCGATTTATCAGACTCATCA
AGTCAGGCCTGGCCAATAAAGCAAGCATCGAGAAAGTGGCTCATATCTTCATCGAAAACTTCGCCAGTAAGGTCAAGTTT
GAAGATGTTAAGGACATGACCATAAGAACATCAGGCAAACTCATCGGCAAATTCGCCTTTAATCAGTTAGCCACCGCAAA
CATGGGATATATATTCTCTAAACAAATCATCCCACGGCTTGTCTCCGGTGTTGCCGTTGGCTCAATTCTCACCGCTGGGG
CAAGCATATCGCGCTCTGTCTATACATCACGCGACTTGAGGGCAAGAAACTCACGGATGTATGATCAACTCAGACGCCTG
GGAAACCTCGATCTGCTGTACTTTCTGTTAGAAAACAAAGTCCGACCATTCGAGGATGCCGTCTCGGTTTGGGAAAAAGA
TCGAAAGAAATTCAGCCAAATTTGTCAGTACTTTTTTGCAAAGGTTAACGTATGA