Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00316  |
| Cognate effector | EFF01501 |
| Related T6SS (Type) | T6SS00929 (Type i3) |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon | chromosome |
| Sequence | Protein sequence (258 a.a.); Nucleotide sequence (774 bp) |
| Description | Immune toxin SMDB11_0927 (Slp,Secreted lipase-like protein) with SMDB11_0929. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | SMDB11_0928 |
| Coordinates (Strand) | 972883..973656 (+) |
| NCBI ID | 560172776 |
| GenBank | HG326223 |
| Uniprot ID | UPI0003257467 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-257 | MKKLILIITMVVSTLIMQGCEKGMDLQPQDYFDGQQLDIAKAIYDGDRPQLDKQLSSVNKEILNRPAKEEMTLLFWAINNAIYDKTTPERLKIITDLVKAGADPLQPQPNTPGSPAEFVMKADKGIWIQAMLEGGLSPNARDKVHNQPIIFNSIFAKNTETLEVMLEHGADINIRNSLGDTLLIDALDYHSYDHVILLLEKGADSDIRGNSGWTMGNQLQRLINRSQDGSEAKESLERIRDELIKRGGKWPPAPVSQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9989 | pos: 19-20. MQG-CE. | MKKLILIITMVVSTLIMQG |
Protein sequence: 258 a.a. .
>IMU00316 HG326223:972883-973656 [Serratia marcescens subsp. marcescens Db11]
MKKLILIITMVVSTLIMQGCEKGMDLQPQDYFDGQQLDIAKAIYDGDRPQLDKQLSSVNKEILNRPAKEEMTLLFWAINN
AIYDKTTPERLKIITDLVKAGADPLQPQPNTPGSPAEFVMKADKGIWIQAMLEGGLSPNARDKVHNQPIIFNSIFAKNTE
TLEVMLEHGADINIRNSLGDTLLIDALDYHSYDHVILLLEKGADSDIRGNSGWTMGNQLQRLINRSQDGSEAKESLERIR
DELIKRGGKWPPAPVSQ*
MKKLILIITMVVSTLIMQGCEKGMDLQPQDYFDGQQLDIAKAIYDGDRPQLDKQLSSVNKEILNRPAKEEMTLLFWAINN
AIYDKTTPERLKIITDLVKAGADPLQPQPNTPGSPAEFVMKADKGIWIQAMLEGGLSPNARDKVHNQPIIFNSIFAKNTE
TLEVMLEHGADINIRNSLGDTLLIDALDYHSYDHVILLLEKGADSDIRGNSGWTMGNQLQRLINRSQDGSEAKESLERIR
DELIKRGGKWPPAPVSQ*
Nucleotide sequence: 774 bp .
>IMU00316 HG326223:972883-973656 [Serratia marcescens subsp. marcescens Db11]
ATGAAAAAACTTATTCTCATTATCACGATGGTAGTGTCTACGCTCATCATGCAAGGGTGCGAAAAAGGTATGGATTTACA
ACCGCAGGATTATTTTGACGGTCAGCAATTGGACATTGCCAAAGCCATTTATGATGGTGATAGGCCGCAGTTGGATAAAC
AGTTATCATCAGTAAACAAAGAGATCTTAAATCGTCCAGCTAAGGAAGAAATGACCTTATTGTTCTGGGCAATAAACAAT
GCCATCTATGATAAAACCACACCTGAAAGGCTGAAAATTATCACTGATTTGGTAAAAGCCGGCGCAGATCCTTTACAGCC
TCAACCCAACACGCCAGGTAGCCCAGCTGAATTTGTTATGAAAGCGGACAAAGGCATCTGGATACAGGCTATGTTAGAGG
GCGGACTTTCTCCAAATGCTAGGGATAAGGTTCATAATCAACCCATTATATTTAATAGCATCTTTGCAAAAAACACAGAA
ACATTAGAGGTCATGTTGGAGCATGGTGCTGATATAAATATAAGAAATTCACTGGGTGATACGCTGCTCATCGATGCTTT
AGACTATCACTCCTATGACCACGTCATTCTTTTATTAGAAAAAGGCGCCGATAGCGATATAAGAGGCAACTCGGGTTGGA
CGATGGGCAATCAACTTCAACGGCTGATTAATAGGAGTCAGGACGGCAGTGAGGCGAAAGAGTCTCTTGAGCGCATCAGA
GACGAGCTAATTAAGCGTGGCGGGAAATGGCCGCCTGCTCCTGTCAGTCAATAG
ATGAAAAAACTTATTCTCATTATCACGATGGTAGTGTCTACGCTCATCATGCAAGGGTGCGAAAAAGGTATGGATTTACA
ACCGCAGGATTATTTTGACGGTCAGCAATTGGACATTGCCAAAGCCATTTATGATGGTGATAGGCCGCAGTTGGATAAAC
AGTTATCATCAGTAAACAAAGAGATCTTAAATCGTCCAGCTAAGGAAGAAATGACCTTATTGTTCTGGGCAATAAACAAT
GCCATCTATGATAAAACCACACCTGAAAGGCTGAAAATTATCACTGATTTGGTAAAAGCCGGCGCAGATCCTTTACAGCC
TCAACCCAACACGCCAGGTAGCCCAGCTGAATTTGTTATGAAAGCGGACAAAGGCATCTGGATACAGGCTATGTTAGAGG
GCGGACTTTCTCCAAATGCTAGGGATAAGGTTCATAATCAACCCATTATATTTAATAGCATCTTTGCAAAAAACACAGAA
ACATTAGAGGTCATGTTGGAGCATGGTGCTGATATAAATATAAGAAATTCACTGGGTGATACGCTGCTCATCGATGCTTT
AGACTATCACTCCTATGACCACGTCATTCTTTTATTAGAAAAAGGCGCCGATAGCGATATAAGAGGCAACTCGGGTTGGA
CGATGGGCAATCAACTTCAACGGCTGATTAATAGGAGTCAGGACGGCAGTGAGGCGAAAGAGTCTCTTGAGCGCATCAGA
GACGAGCTAATTAAGCGTGGCGGGAAATGGCCGCCTGCTCCTGTCAGTCAATAG