Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00031  |
| Type | adaptor |
| Related T6SS (Type) | T6SS00929 (Type i3) |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon | chromosome |
| Related structure protein | SMDB11_2276 (VgrG2b) |
| Related effector | SMDB11_2278 (Rhs1) |
| Description | - |
| Reference | [1] Cianfanelli FR, Alcoforado Diniz J, Guo M, et al. VgrG and PAAR Proteins Define Distinct Versions of a Functional Type VI Secretion System. PLoS Pathog. 2016 Jun 28;12(6):e1005735. doi: 10.1371/journal.ppat.1005735. eCollection 2016 Jun. PMID: 27352036 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | SMDB11_2245 (eagR1) |
| Coordinates (Strand) | 2367474..2367863 (+) |
| NCBI ID | 560174088 |
| GenBank | HG326223 |
| Uniprot ID | UPI000322B1F1 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF3592 | PF12158.10 | 1.3e-05 | 15..124 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MSKAKN |
| Transmembrane helix | 7-29 | VFTVIGLILLAPILSLVGFFKFI |
| Outside | 30-129 | TVDIWGYFKHIGSSLSVMENGISASGQIVSIRQTNLWDGNRPVCEVEVKYIAQDGKNHTAVAKGPISVVDLPRYQPGHFTAIKYDPKNPKKAVIEELSRL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9885 | - | - |
Protein sequence: 130 a.a.
>ACP00031 HG326223:2367474-2367863 [Serratia marcescens subsp. marcescens Db11]
MSKAKNVFTVIGLILLAPILSLVGFFKFITVDIWGYFKHIGSSLSVMENGISASGQIVSIRQTNLWDGNRPVCEVEVKYI
AQDGKNHTAVAKGPISVVDLPRYQPGHFTAIKYDPKNPKKAVIEELSRL*
MSKAKNVFTVIGLILLAPILSLVGFFKFITVDIWGYFKHIGSSLSVMENGISASGQIVSIRQTNLWDGNRPVCEVEVKYI
AQDGKNHTAVAKGPISVVDLPRYQPGHFTAIKYDPKNPKKAVIEELSRL*
Nucleotide sequence: 390 bp
>ACP00031 HG326223:2367474-2367863 [Serratia marcescens subsp. marcescens Db11]
ATGTCGAAGGCCAAAAATGTTTTCACCGTTATCGGGTTGATTTTATTGGCTCCCATCCTCAGTCTGGTGGGTTTTTTCAA
ATTCATCACGGTCGATATCTGGGGTTATTTCAAACATATCGGTTCTTCCCTCAGCGTGATGGAGAACGGTATTTCCGCTT
CCGGGCAAATCGTCAGCATTCGCCAGACCAACCTGTGGGACGGAAATCGGCCGGTCTGCGAAGTCGAAGTCAAGTACATC
GCCCAAGACGGAAAAAACCATACGGCCGTGGCGAAAGGCCCGATCAGCGTCGTGGATCTGCCGCGTTATCAGCCGGGCCA
TTTCACCGCGATAAAGTACGATCCGAAGAACCCCAAAAAAGCGGTGATCGAAGAGCTTTCCCGGCTTTGA
ATGTCGAAGGCCAAAAATGTTTTCACCGTTATCGGGTTGATTTTATTGGCTCCCATCCTCAGTCTGGTGGGTTTTTTCAA
ATTCATCACGGTCGATATCTGGGGTTATTTCAAACATATCGGTTCTTCCCTCAGCGTGATGGAGAACGGTATTTCCGCTT
CCGGGCAAATCGTCAGCATTCGCCAGACCAACCTGTGGGACGGAAATCGGCCGGTCTGCGAAGTCGAAGTCAAGTACATC
GCCCAAGACGGAAAAAACCATACGGCCGTGGCGAAAGGCCCGATCAGCGTCGTGGATCTGCCGCGTTATCAGCCGGGCCA
TTTCACCGCGATAAAGTACGATCCGAAGAACCCCAAAAAAGCGGTGATCGAAGAGCTTTCCCGGCTTTGA