SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01500
Effector type	Tse
Cognate immunity protein	IMU00319
Related T6SS (Type)	T6SS00929 (Type i3)
Strain	Serratia marcescens subsp. marcescens Db11
Replicon	chromosome
Sequence	Protein sequence (1474 a.a.); Nucleotide sequence (4422 bp)
Description	Rhs1 represents a novel antibacterial toxin, active in the cytoplasm of target cells and neutralized by RhsI1
Reference	[1] Alcoforado Diniz J, Coulthurst SJ. Intraspecies Competition in Serratia marcescens Is Mediated by Type VI-Secreted Rhs Effectors and a Conserved Effector-Associated Accessory Protein. J Bacteriol. 2015 Jul;197(14):2350-60. doi: 10.1128/JB.00199-15. Epub 2015 May 4. PMID: 25939831 [2] Cianfanelli FR, Alcoforado Diniz J, Guo M, et al. VgrG and PAAR Proteins Define Distinct Versions of a Functional Type VI Secretion System. PLoS Pathog. 2016 Jun 28;12(6):e1005735. doi: 10.1371/journal.ppat.1005735. eCollection 2016 Jun. PMID: 27352036

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	SMDB11_2278 (Rhs1)
Coordinates (Strand)	2403168..2407589 (+)
NCBI ID	560174121
GenBank	HG326223
Uniprot ID	UPI0003247414
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DUF6531	PF20148.1	4.7e-19	295..373
RHS	PF03527.16	8.8e-10	1226..1259
RHS_repeat	PF05593.16	4.3e-09	720..755
PAAR_motif	PF05488.15	8.6e-08	180..221
RHS_repeat	PF05593.16	1.9e-07	783..817
RHS_repeat	PF05593.16	1e-05	923..959

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-21	MAEIWAARKDDELIHTSALAD
Transmembrane helix	22-44	IASAVAEVAVYAVATAAVSAAAA
Inside	45-50	AAAPAL
Transmembrane helix	51-73	AGVAVLGAAATAASGCVGAGLIV
Outside	74-1473	GFATSLTGVGEHISNGCDAVANWLFPPSVSGVILTGSPNVFTNAKPAARAAGRLLTPAEIKALPAPQPPQSFWDYGAELLAAGGNILSQLWQPGVAGPSQPSQPLEEDKISCDKHPLPQYLAEGSSKVFINGQPAVRSNDRSTCEAKVSTDVSKNVQIGGGTVVVRDIKSGKLPGIGLALTVLPLLLGNPAKLVKNLPCMIGGGLAAAGVNALGEAIHAAFNPVHAATGAKVLNDEEDLDLLLPARFPLIWQRNYNSRNGYQGLFGHGWRTPFEIWVDLNDRDGVIECCLHDETGRELRFPLPEVNQPSFSAGEGLMFRRGEEGQLLIADTDGSVWRLFVPLPQTPSRLRLVSLSDEYDNALLLNYDERQRLSHIVDSAGTMTLRLEYGDPRFDDRVTALRHNDEPRVRYRYDGGGDLHQVIDAGGLVTREFHYNAQHLLTRHQQAGGPSFAYRWAMFDDWRVVEHRDSAGGGCTIDYDLAAGLTTVTEYDGLRHQHRWNAQRLIVEYTDERGEVWHYQWNDEDMLIATRDPLGHGWHFRYDEAGNLVEEEDPLGHIRLTGWLPQRALPISVKEPNGATQHFYYDERHALIAEIDALGHTQRYLRDGYGQVVQAVDANGGISHFAYDEHGQLLRARDCSGKQTLYKYTAQGWLSEVTDAAGERVHYRYDAAGRLTQLQRAEGWQETLSWNAQGQLDRYQGADGRLTHYRYDAAGRLLATRNPLDGEVVRHYDARGRLTALRNENAESYLFHWTACNLLQEEIGLDGVSTRYRYDECRRVVERRFAANTSAAFSHYFSHDGAGQLVSKQTPDGETRYRYSPLGQLLHAASYRGNAWHDEPTTAQSEVSFDYDLLGRVTREQGANGTLRYDYDPMGNRTQTTLPDGRTLRALYYGSGHLLQLALDEITLSEFERDNLHRELSRTQGRLTSRVHYDRLGRKDRREIFNDNRQRPAPASSSRQWSYDYQNNLTREEHNANPFSYQRYQYDATGRLLSRDGTRPDTERYRYDPASNLLDEGQFRQRHNRVLHYQGIDYRYDVFGRTVEKRKGHYRWQYLYDADHRLCEVVRYSQHRSEAQQHVHFHYDPLGRRTQKRVWQQSQDLRQPAGKCQTTTFLWEGFRLLQETRDGMPLTYVYADQGSYEPLARIDGHAPAQVFYFHTAPNGEPESLTDSDGTLRWQSHSSAWGRIKYEENQQDLDYSQNLRLQGQYLDRETGLHYNLFRYYDPDIGRFTQHDPIGLAGGINLYQYAPNPLGWVDPWGWKPRCAATKANDHNQAAFGRQWQGRGIYKGRDSWSNIMLKEGDIVYGGAPGQSGFYFNKATLDAAGGSRAKLWESLQVLPHEKFGYRSKIQAYRVKRETIAGTGKAISQDPTRFGEGGGTQFFLSNYKTVLEPIDKPFEIGL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.4744	-	-

Protein sequence: 1474 a.a. .

                                    >EFF01500 HG326223:2403168-2407589 Type VI Secretion System Effector (Tse) [Serratia marcescens subsp. marcescens Db11]
MAEIWAARKDDELIHTSALADIASAVAEVAVYAVATAAVSAAAAAAAPALAGVAVLGAAATAASGCVGAGLIVGFATSLT
GVGEHISNGCDAVANWLFPPSVSGVILTGSPNVFTNAKPAARAAGRLLTPAEIKALPAPQPPQSFWDYGAELLAAGGNIL
SQLWQPGVAGPSQPSQPLEEDKISCDKHPLPQYLAEGSSKVFINGQPAVRSNDRSTCEAKVSTDVSKNVQIGGGTVVVRD
IKSGKLPGIGLALTVLPLLLGNPAKLVKNLPCMIGGGLAAAGVNALGEAIHAAFNPVHAATGAKVLNDEEDLDLLLPARF
PLIWQRNYNSRNGYQGLFGHGWRTPFEIWVDLNDRDGVIECCLHDETGRELRFPLPEVNQPSFSAGEGLMFRRGEEGQLL
IADTDGSVWRLFVPLPQTPSRLRLVSLSDEYDNALLLNYDERQRLSHIVDSAGTMTLRLEYGDPRFDDRVTALRHNDEPR
VRYRYDGGGDLHQVIDAGGLVTREFHYNAQHLLTRHQQAGGPSFAYRWAMFDDWRVVEHRDSAGGGCTIDYDLAAGLTTV
TEYDGLRHQHRWNAQRLIVEYTDERGEVWHYQWNDEDMLIATRDPLGHGWHFRYDEAGNLVEEEDPLGHIRLTGWLPQRA
LPISVKEPNGATQHFYYDERHALIAEIDALGHTQRYLRDGYGQVVQAVDANGGISHFAYDEHGQLLRARDCSGKQTLYKY
TAQGWLSEVTDAAGERVHYRYDAAGRLTQLQRAEGWQETLSWNAQGQLDRYQGADGRLTHYRYDAAGRLLATRNPLDGEV
VRHYDARGRLTALRNENAESYLFHWTACNLLQEEIGLDGVSTRYRYDECRRVVERRFAANTSAAFSHYFSHDGAGQLVSK
QTPDGETRYRYSPLGQLLHAASYRGNAWHDEPTTAQSEVSFDYDLLGRVTREQGANGTLRYDYDPMGNRTQTTLPDGRTL
RALYYGSGHLLQLALDEITLSEFERDNLHRELSRTQGRLTSRVHYDRLGRKDRREIFNDNRQRPAPASSSRQWSYDYQNN
LTREEHNANPFSYQRYQYDATGRLLSRDGTRPDTERYRYDPASNLLDEGQFRQRHNRVLHYQGIDYRYDVFGRTVEKRKG
HYRWQYLYDADHRLCEVVRYSQHRSEAQQHVHFHYDPLGRRTQKRVWQQSQDLRQPAGKCQTTTFLWEGFRLLQETRDGM
PLTYVYADQGSYEPLARIDGHAPAQVFYFHTAPNGEPESLTDSDGTLRWQSHSSAWGRIKYEENQQDLDYSQNLRLQGQY
LDRETGLHYNLFRYYDPDIGRFTQHDPIGLAGGINLYQYAPNPLGWVDPWGWKPRCAATKANDHNQAAFGRQWQGRGIYK
GRDSWSNIMLKEGDIVYGGAPGQSGFYFNKATLDAAGGSRAKLWESLQVLPHEKFGYRSKIQAYRVKRETIAGTGKAISQ
DPTRFGEGGGTQFFLSNYKTVLEPIDKPFEIGL*

                                

Nucleotide sequence: 4422 bp .

                                    >EFF01500 HG326223:2403168-2407589 Type VI Secretion System Effector (Tse) [Serratia marcescens subsp. marcescens Db11]
ATGGCTGAGATCTGGGCAGCAAGAAAGGATGATGAACTGATCCACACCAGCGCGTTGGCGGACATCGCCAGCGCCGTCGC
CGAAGTCGCGGTGTATGCGGTGGCGACCGCCGCCGTGTCCGCAGCAGCCGCCGCCGCAGCGCCGGCATTGGCAGGGGTGG
CGGTGCTCGGCGCAGCGGCGACGGCGGCCTCCGGCTGCGTCGGCGCCGGGCTGATCGTCGGTTTTGCCACCAGCCTGACC
GGCGTCGGAGAACATATCAGCAACGGCTGCGACGCCGTGGCCAACTGGCTCTTCCCGCCTTCCGTCTCCGGCGTCATTCT
CACGGGCTCCCCCAACGTCTTCACCAACGCCAAACCGGCGGCACGCGCCGCAGGTCGTTTACTCACGCCCGCAGAGATCA
AAGCGTTACCGGCGCCACAGCCGCCGCAAAGCTTCTGGGATTATGGCGCCGAGCTGCTGGCCGCCGGCGGTAATATCCTC
TCGCAGCTATGGCAGCCCGGCGTCGCCGGCCCCAGCCAGCCCAGCCAACCGCTGGAAGAAGATAAAATCAGCTGCGACAA
ACACCCGCTTCCGCAATACCTGGCGGAAGGCTCCAGTAAAGTATTTATCAACGGCCAACCGGCCGTACGCAGCAATGACC
GTTCAACCTGCGAGGCGAAAGTCTCGACCGACGTCTCCAAAAATGTGCAGATCGGCGGCGGCACGGTGGTGGTACGCGAC
ATCAAGAGCGGCAAGCTGCCGGGTATCGGTTTGGCGCTGACCGTGCTGCCGCTGCTGCTCGGTAATCCGGCCAAGCTGGT
TAAGAACCTGCCTTGCATGATCGGCGGCGGTCTGGCGGCGGCGGGCGTTAACGCCCTCGGCGAAGCGATCCACGCCGCCT
TTAACCCGGTGCATGCCGCCACCGGCGCCAAGGTATTGAACGACGAAGAAGATCTGGACCTGTTGCTGCCGGCGCGCTTT
CCGCTGATCTGGCAGCGCAATTACAACAGCCGCAATGGCTATCAGGGACTGTTTGGCCACGGCTGGCGCACGCCTTTTGA
AATTTGGGTCGATCTTAACGACCGGGACGGCGTCATTGAGTGTTGCCTGCATGATGAAACCGGTCGTGAGCTACGCTTCC
CGCTGCCTGAGGTGAACCAGCCGAGCTTCAGCGCCGGTGAAGGGCTGATGTTCCGCCGCGGTGAAGAGGGACAGCTGCTG
ATCGCCGACACCGACGGCAGCGTCTGGCGGCTGTTCGTGCCGCTGCCGCAGACCCCTTCCCGCCTGAGGCTGGTGTCGTT
GAGCGATGAGTACGATAATGCGCTGCTGCTGAACTACGACGAACGACAGCGCCTGAGCCACATCGTCGACAGCGCCGGCA
CCATGACATTGCGGCTGGAATACGGCGATCCGCGCTTCGACGATCGCGTCACCGCCTTGCGGCATAACGACGAACCACGC
GTGCGCTACCGTTATGACGGCGGCGGCGATCTGCATCAGGTGATCGACGCCGGCGGCTTGGTCACGCGTGAATTTCACTA
TAACGCCCAACATCTATTGACCCGCCACCAACAGGCCGGTGGGCCCAGCTTCGCGTATCGCTGGGCGATGTTCGACGACT
GGCGCGTGGTGGAACACCGCGACAGCGCCGGCGGCGGCTGTACAATCGACTACGATTTGGCCGCCGGGTTAACGACGGTC
ACCGAGTATGACGGCTTGCGGCACCAGCACCGCTGGAACGCCCAACGGCTGATCGTCGAATATACCGACGAGCGTGGTGA
GGTCTGGCATTACCAATGGAATGACGAAGACATGCTGATCGCCACGCGCGATCCGCTCGGCCACGGCTGGCATTTCCGCT
ACGATGAAGCCGGTAATCTGGTCGAGGAAGAGGATCCGCTCGGCCATATCCGCCTCACCGGTTGGCTACCGCAGCGCGCA
CTGCCGATCAGCGTCAAGGAGCCCAACGGCGCCACACAGCATTTCTACTACGACGAGCGCCATGCCTTGATTGCCGAGAT
CGATGCGCTCGGCCATACCCAGCGCTACCTGCGCGATGGCTATGGCCAGGTGGTGCAAGCCGTCGACGCCAACGGCGGCA
TCAGCCATTTCGCTTATGATGAGCACGGGCAATTATTGCGCGCCCGCGATTGCTCCGGCAAACAAACCCTCTACAAATAT
ACGGCTCAAGGCTGGCTCAGCGAGGTGACCGACGCCGCCGGCGAACGCGTCCATTACCGCTACGATGCCGCCGGCCGCCT
GACACAGCTGCAGCGCGCCGAGGGCTGGCAAGAGACGCTGAGCTGGAACGCGCAGGGCCAGCTGGATCGCTATCAGGGGG
CCGATGGGCGCCTGACGCATTATCGCTATGACGCAGCCGGCCGCCTGCTGGCCACGCGCAATCCGCTCGATGGCGAGGTG
GTGCGCCACTACGACGCCCGCGGCAGATTAACGGCGCTGCGCAATGAGAATGCCGAAAGCTACCTGTTCCATTGGACGGC
CTGCAATCTGTTGCAGGAAGAAATCGGGTTGGACGGCGTCAGCACCCGCTATCGTTACGACGAATGCCGCCGGGTCGTCG
AGCGCCGCTTCGCCGCCAATACCTCCGCCGCCTTCTCGCACTATTTCAGCCATGACGGCGCCGGCCAACTGGTGAGCAAA
CAGACGCCCGATGGAGAAACCCGCTACCGCTACAGTCCGCTCGGCCAACTGCTGCATGCCGCCAGCTACCGCGGCAACGC
TTGGCATGACGAGCCGACAACGGCTCAGAGCGAGGTCAGTTTTGACTATGACTTGCTGGGGCGGGTGACCCGTGAGCAAG
GTGCCAACGGTACGCTACGCTATGATTACGATCCGATGGGTAACCGCACGCAAACCACCTTGCCCGACGGACGCACGCTG
CGCGCACTGTACTACGGTAGCGGCCACCTGCTGCAGCTGGCGCTGGACGAGATCACCCTCAGCGAGTTCGAGCGCGACAA
TCTGCACCGCGAACTGAGCCGTACGCAGGGTCGCCTGACCAGCCGAGTGCATTATGATCGGCTGGGGCGCAAAGACCGGC
GGGAGATCTTCAATGACAACCGCCAGCGCCCGGCGCCGGCCAGCAGCTCGCGCCAGTGGTCTTACGACTATCAAAACAAC
CTGACGCGCGAAGAACACAACGCCAACCCGTTCTCCTACCAGCGTTATCAGTACGATGCCACCGGTCGATTGCTGAGCCG
GGACGGCACGCGGCCGGATACCGAGCGCTACCGGTACGATCCGGCATCCAACCTGCTGGATGAGGGGCAATTCCGCCAGC
GGCACAACCGCGTACTGCATTATCAGGGAATTGATTACCGGTACGATGTCTTTGGCCGCACGGTGGAAAAACGCAAAGGC
CACTACCGTTGGCAATATCTCTACGACGCCGATCATCGCCTCTGCGAGGTGGTGCGCTACAGCCAGCATCGCAGCGAAGC
CCAGCAGCATGTGCATTTCCACTATGATCCGCTCGGGCGGCGCACGCAAAAGCGGGTGTGGCAGCAAAGTCAGGATCTGC
GCCAACCGGCCGGCAAATGCCAAACCACCACGTTTTTGTGGGAAGGTTTCCGGCTGCTGCAGGAAACTCGCGACGGCATG
CCCCTCACCTATGTCTATGCCGATCAGGGCAGCTATGAACCTTTGGCGCGCATCGACGGCCACGCGCCGGCCCAAGTATT
TTACTTCCACACGGCGCCGAACGGCGAACCGGAAAGCCTGACCGACAGCGACGGAACGCTGCGCTGGCAGAGCCACAGCA
GCGCCTGGGGCCGCATAAAGTATGAGGAAAATCAGCAAGATCTGGATTACTCCCAAAACCTGCGCCTGCAGGGGCAATAC
CTGGATCGGGAAACGGGATTGCACTACAATTTGTTCCGCTATTACGATCCGGATATCGGCAGATTTACCCAGCACGATCC
GATAGGTTTGGCCGGCGGGATCAACCTGTACCAATACGCGCCGAATCCGCTGGGTTGGGTGGATCCTTGGGGATGGAAGC
CACGATGCGCAGCAACCAAAGCCAACGATCATAATCAGGCAGCTTTTGGTCGACAATGGCAAGGTCGAGGTATCTACAAA
GGACGAGACTCTTGGTCAAATATCATGTTGAAAGAAGGTGACATTGTTTATGGCGGCGCTCCAGGGCAATCTGGTTTTTA
CTTCAACAAGGCGACGCTAGATGCTGCAGGTGGCAGTAGAGCCAAGCTATGGGAAAGTTTGCAGGTGCTTCCACATGAAA
AATTTGGTTATAGATCTAAAATACAAGCATATAGAGTAAAAAGAGAAACGATTGCAGGAACAGGTAAAGCGATATCTCAA
GACCCAACGAGATTCGGCGAAGGCGGAGGAACTCAATTTTTCCTTTCTAACTATAAAACTGTTCTTGAGCCAATTGATAA
GCCTTTTGAAATTGGATTGTAA

                                