SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0017
Regulator type	protein
Regulatory pathway (from literatures)	Tpp
KEGG pathway (determined by BlastKOALA)	Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS	T6SS00929 (Type i3)
Strain	Serratia marcescens subsp. marcescens Db11
Replicon	chromosome
Sequence	Protein sequence (240 a.a.); Nucleotide sequence (720 bp)
Description	TagF negatively regulates assembly of a functional T6SS and that PpkA-mediated phosphorylation is required to overcome this repression.
Reference	[1] Ostrowski A, Cianfanelli FR, Porter M, et al. Killing with proficiency: Integrated post-translational regulation of an offensive Type VI secretion system. PLoS Pathog. 2018 Jul 27;14(7):e1007230. doi: 10.1371/journal.ppat.1007230. eCollection 2018 Jul. PMID: 30052683

External database links

Locus tag (Gene)	SMDB11_2256 (TagF)
Coordinates (Strand)	2380702..2381421 (+)
NCBI ID	560174099
GenBank	HG326223
Uniprot ID	UPI0003C9F165
KEGG ID	-
KEGG pathway (determined by BlastKOALA)	map02025
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
Unknown	↑	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
TagF_N	PF09867.11	8.6e-40	12..146

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-239	MSTDSSAPTSIGWYGKLPSAGDFLQRRLPDPVVNNWAHWFHNGLVNLQRDAQGPNGHPFSNAPVWNFVIPATLGSPYVQMGCLLPARDRVGRRYPICALRLFSLQEWRPQQLNMAASWYQQLGHTLLNGVRNGFSAEQIDRTLQAIPALPSPPAEADSEILSIIGFQHPDVPGLGWQQAADCFDPAQYTSFWWTNQADGHPLYTHVHSGNLTVQLFSLLFEPNGWARPGRGGQYPQMFD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9784	-	-

Protein sequence of regulator: 240 a.a. .

                                    >REG0017 HG326223:2380702-2381421 [Serratia marcescens subsp. marcescens Db11]
MSTDSSAPTSIGWYGKLPSAGDFLQRRLPDPVVNNWAHWFHNGLVNLQRDAQGPNGHPFSNAPVWNFVIPATLGSPYVQM
GCLLPARDRVGRRYPICALRLFSLQEWRPQQLNMAASWYQQLGHTLLNGVRNGFSAEQIDRTLQAIPALPSPPAEADSEI
LSIIGFQHPDVPGLGWQQAADCFDPAQYTSFWWTNQADGHPLYTHVHSGNLTVQLFSLLFEPNGWARPGRGGQYPQMFD*

                                 

Nucleotide sequence of regulator: 720 bp .

                                    >REG0017 HG326223:2380702-2381421 [Serratia marcescens subsp. marcescens Db11]
ATGAGCACTGATTCAAGCGCCCCGACGAGCATCGGCTGGTACGGCAAACTTCCTTCCGCCGGTGACTTCCTGCAACGCCG
TTTGCCTGACCCGGTGGTCAACAACTGGGCCCACTGGTTCCATAACGGGCTGGTGAATTTACAGCGCGATGCACAGGGGC
CGAACGGCCACCCGTTCAGCAACGCACCGGTGTGGAACTTCGTGATCCCCGCCACGCTCGGCAGCCCGTACGTGCAGATG
GGTTGCCTGTTGCCGGCGCGCGATCGCGTCGGGCGGCGCTACCCCATCTGCGCACTGCGCCTGTTCAGCCTGCAAGAATG
GCGCCCGCAACAGCTGAACATGGCGGCGAGCTGGTATCAACAGCTCGGCCATACCCTGCTGAACGGCGTGCGCAACGGTT
TCTCCGCCGAGCAGATCGATCGCACGTTGCAGGCGATCCCGGCGCTGCCCAGCCCACCGGCGGAAGCGGATTCCGAGATA
TTGTCGATTATCGGTTTCCAGCATCCGGACGTACCGGGCCTCGGCTGGCAACAGGCCGCCGACTGCTTCGATCCGGCGCA
GTACACCAGCTTCTGGTGGACCAATCAGGCCGACGGACATCCGCTCTACACCCACGTGCACAGCGGCAACCTCACCGTGC
AGCTGTTTTCACTGCTGTTCGAACCCAACGGCTGGGCGCGCCCCGGCCGCGGCGGCCAGTACCCGCAAATGTTTGATTAA

                                