SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00320
Cognate effector	EFF01499
Related T6SS (Type)	T6SS00929 (Type i3)
Strain	Serratia marcescens subsp. marcescens Db11
Replicon	chromosome
Sequence	Protein sequence (163 a.a.); Nucleotide sequence (489 bp)
Description	RhsI2 is a direct immunity function for Rhs2
Reference	[1] Alcoforado Diniz J, Coulthurst SJ. Intraspecies Competition in Serratia marcescens Is Mediated by Type VI-Secreted Rhs Effectors and a Conserved Effector-Associated Accessory Protein. J Bacteriol. 2015 Jul;197(14):2350-60. doi: 10.1128/JB.00199-15. Epub 2015 May 4. PMID: 25939831

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	SMDB11_1611 (RhsI2)
Coordinates (Strand)	1693944..1694432 (+)
NCBI ID	560173454
GenBank	HG326223
Uniprot ID	UPI0003C9C41B
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-162	MNEFDFDSLLQRIDSSCFFSRMGLPDVLDSRVILIENVEKVFVNPTDAEFKGYYDSVEWLPTSMTQEDPFYKVKEVLPKELTGLRIRVNKAVMNATKGLSKDKFNYGPHDFSLAARNGICFAFREYVSEQYLHLGNKWEEVVGIYFSGHWPVGIAKDKIVTI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9961	-	-

Protein sequence: 163 a.a. .

                                    >IMU00320 HG326223:1693944-1694432 [Serratia marcescens subsp. marcescens Db11]
MNEFDFDSLLQRIDSSCFFSRMGLPDVLDSRVILIENVEKVFVNPTDAEFKGYYDSVEWLPTSMTQEDPFYKVKEVLPKE
LTGLRIRVNKAVMNATKGLSKDKFNYGPHDFSLAARNGICFAFREYVSEQYLHLGNKWEEVVGIYFSGHWPVGIAKDKIV
TI*

                                

Nucleotide sequence: 489 bp .

                                    >IMU00320 HG326223:1693944-1694432 [Serratia marcescens subsp. marcescens Db11]
ATGAATGAATTTGACTTTGATAGTTTACTACAGCGCATCGATAGCTCATGCTTTTTCTCAAGAATGGGGCTCCCAGATGT
TCTGGATAGTCGCGTGATTTTAATTGAAAATGTTGAGAAGGTGTTTGTTAATCCCACTGATGCAGAGTTTAAGGGATACT
ATGATAGTGTGGAATGGTTGCCAACATCAATGACTCAGGAGGATCCTTTCTACAAAGTAAAGGAGGTATTACCTAAGGAA
CTTACTGGTTTACGTATTCGAGTAAACAAGGCTGTCATGAATGCAACAAAGGGATTGTCTAAGGATAAATTCAATTACGG
GCCTCATGACTTCAGCTTAGCAGCCAGAAATGGAATTTGTTTTGCGTTCAGAGAGTATGTTTCTGAACAGTATCTTCATT
TAGGGAATAAGTGGGAAGAAGTTGTGGGTATATACTTTTCTGGTCACTGGCCTGTGGGTATAGCAAAAGATAAGATAGTT
ACCATTTAA

                                