SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01499
Effector type	Tde
Cognate immunity protein	IMU00320
Related T6SS (Type)	T6SS00929 (Type i3)
Strain	Serratia marcescens subsp. marcescens Db11
Replicon	chromosome
Sequence	Protein sequence (1431 a.a.); Nucleotide sequence (4293 bp)
Description	The Rhs2 C-terminal domain is a DNase toxin and exhibits DNase activity
Reference	[1] Alcoforado Diniz J, Coulthurst SJ. Intraspecies Competition in Serratia marcescens Is Mediated by Type VI-Secreted Rhs Effectors and a Conserved Effector-Associated Accessory Protein. J Bacteriol. 2015 Jul;197(14):2350-60. doi: 10.1128/JB.00199-15. Epub 2015 May 4. PMID: 25939831 [2] Cianfanelli FR, Alcoforado Diniz J, Guo M, et al. VgrG and PAAR Proteins Define Distinct Versions of a Functional Type VI Secretion System. PLoS Pathog. 2016 Jun 28;12(6):e1005735. doi: 10.1371/journal.ppat.1005735. eCollection 2016 Jun. PMID: 27352036

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	SMDB11_1610 (Rhs2)
Coordinates (Strand)	1689650..1693942 (+)
NCBI ID	560173453
GenBank	HG326223
Uniprot ID	UPI000321CA9D
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
PAAR_motif	PF05488.15	8.2e-17	88..154
DUF6531	PF20148.1	1.5e-15	242..316
RHS	PF03527.16	2.2e-11	1178..1211
RHS_repeat	PF05593.16	2.5e-11	640..675
RHS_repeat	PF05593.16	1.5e-06	553..590
RHS_repeat	PF05593.16	8.2e-06	703..735
RHS_repeat	PF05593.16	2.3e-05	402..441

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-14	MTEAARVGDTIGHS
Transmembrane helix	15-37	HALAGMIAGTLVGGLIAAAGALA
Inside	38-41	AGAL
Transmembrane helix	42-64	FVAGLAASCIGVGVLLIGASLAV
Outside	65-1430	GYLTGEAATAARDGIADAGAGSLTPKGNIVTGSPNVFINGKPAALATNSQVACSDDGPSMQMAQGSDKVSINGQPASRVGDKTNCDAQVMEGSPNVFIGGGTVTTLPIKPEVPDWLYKVSDLTLLFAGLVGGVGGAAGKLGALGKLLGKLPGINKLARIACRAGTLMTATAAVGIIARPVDIVSGQKFLDGEDDLDFVLPSRLPVAWQRYWRSGNPGDSVLGRGWNLFWESSLQPYQDGLVWRAPSGDFVAFPMVPRGHKTYCEAEKCWLMHNDDGSWQLFDVGEQIFHYPPLAGEQPSRLSMITDAIGNATSLFYDDEGLLSELVDSAGQRLMCRYAQGRLREVALQTAEGERTLARYGYDEQGQLTTVSNRAGEVTRRFGWRDGLMISHQDAAGLLNEYQWQEIDGVPRVTAYRNSAGESLEFGYDFAGGRRSAVRGDGKRAEWRLDDDDNVAQYTDFDQRRYGFIYQRGELCSVLLPGGAQRQSEWDPYGRLLSETDPLGRVTRYQYSRNSGRLFAVAYPDGSSEAQHWDTLGRPTRYVDALGNTTLYRYPDDEESLPASVIDALGGEVKLEWDARGQLTRYTDCSGSVTAYTYDALGQLTAQTDAEGHQTRYRWDNGGRLHTLIHPDGGEERFNWNAHGQLAEHQDALGSLTRWQYNALGLPVSITDRINRTRRYHYSPQGWLTRLENGNGGDYRFSYDAVGRVVAEERPDDTRHYYRYGAAGLLEEHREVGLPGGAGELTQREQRFRFDEAGQLVWRSNASAEWHYRFDAMGRLRELNRLPTASGVALGIEPDSVQMRYDAAGRLLGEQGVNGELQYQWDALANLQALTLPQGDRLQWLYYGSGHASAIKFNQQVVSEFTRDRLHRETGRSQGALQQQRRYDAMGRRSWQSSAFGHDKLTRPEDGVLWRAYRYTGRGELAGVSDALRGEVHYGYDAEGRLLQHREPNQGKPGARLVYDLADNLLGERSPQSDIDAHLPLAPIADNRLTHWQKLFYRYDAWGNLISRRNGLYEQHYRYDADNRLVQAHGRGPQGEFEAQYHYDALGRRSRKTVHYKGKTEQTTHFLWQGYRLLQEQRDDGSRRSWSYDPASPWSPLAALEQAGDSRSADIYWYHTDLNSAPLEVTDAAGNLCWSGQYDTFGKLQGQTVAGAAKRQGAQYQQPLRYAGQYQDDESGLHYNLFRYYEPEVGRFTTQDPIGLEGGLNLYAYGPNPLTWIDPFGLSNCSTLDRIIGDANKVASRGGAITAKQAQILRDNLPVVQRRSVFQNQMARKEFVRDQHYLMSQWEANTGRTWPTGATPHHIIPLESGGANKWWNLMPTHGTLPNHSLPGVPGPHAAGGVLRTTVQQSRKALPPGTITDLRL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9300	-	-

Protein sequence: 1431 a.a. .

                                    >EFF01499 HG326223:1689650-1693942 Type VI DNase and RNase Effector (Tde) [Serratia marcescens subsp. marcescens Db11]
MTEAARVGDTIGHSHALAGMIAGTLVGGLIAAAGALAAGALFVAGLAASCIGVGVLLIGASLAVGYLTGEAATAARDGIA
DAGAGSLTPKGNIVTGSPNVFINGKPAALATNSQVACSDDGPSMQMAQGSDKVSINGQPASRVGDKTNCDAQVMEGSPNV
FIGGGTVTTLPIKPEVPDWLYKVSDLTLLFAGLVGGVGGAAGKLGALGKLLGKLPGINKLARIACRAGTLMTATAAVGII
ARPVDIVSGQKFLDGEDDLDFVLPSRLPVAWQRYWRSGNPGDSVLGRGWNLFWESSLQPYQDGLVWRAPSGDFVAFPMVP
RGHKTYCEAEKCWLMHNDDGSWQLFDVGEQIFHYPPLAGEQPSRLSMITDAIGNATSLFYDDEGLLSELVDSAGQRLMCR
YAQGRLREVALQTAEGERTLARYGYDEQGQLTTVSNRAGEVTRRFGWRDGLMISHQDAAGLLNEYQWQEIDGVPRVTAYR
NSAGESLEFGYDFAGGRRSAVRGDGKRAEWRLDDDDNVAQYTDFDQRRYGFIYQRGELCSVLLPGGAQRQSEWDPYGRLL
SETDPLGRVTRYQYSRNSGRLFAVAYPDGSSEAQHWDTLGRPTRYVDALGNTTLYRYPDDEESLPASVIDALGGEVKLEW
DARGQLTRYTDCSGSVTAYTYDALGQLTAQTDAEGHQTRYRWDNGGRLHTLIHPDGGEERFNWNAHGQLAEHQDALGSLT
RWQYNALGLPVSITDRINRTRRYHYSPQGWLTRLENGNGGDYRFSYDAVGRVVAEERPDDTRHYYRYGAAGLLEEHREVG
LPGGAGELTQREQRFRFDEAGQLVWRSNASAEWHYRFDAMGRLRELNRLPTASGVALGIEPDSVQMRYDAAGRLLGEQGV
NGELQYQWDALANLQALTLPQGDRLQWLYYGSGHASAIKFNQQVVSEFTRDRLHRETGRSQGALQQQRRYDAMGRRSWQS
SAFGHDKLTRPEDGVLWRAYRYTGRGELAGVSDALRGEVHYGYDAEGRLLQHREPNQGKPGARLVYDLADNLLGERSPQS
DIDAHLPLAPIADNRLTHWQKLFYRYDAWGNLISRRNGLYEQHYRYDADNRLVQAHGRGPQGEFEAQYHYDALGRRSRKT
VHYKGKTEQTTHFLWQGYRLLQEQRDDGSRRSWSYDPASPWSPLAALEQAGDSRSADIYWYHTDLNSAPLEVTDAAGNLC
WSGQYDTFGKLQGQTVAGAAKRQGAQYQQPLRYAGQYQDDESGLHYNLFRYYEPEVGRFTTQDPIGLEGGLNLYAYGPNP
LTWIDPFGLSNCSTLDRIIGDANKVASRGGAITAKQAQILRDNLPVVQRRSVFQNQMARKEFVRDQHYLMSQWEANTGRT
WPTGATPHHIIPLESGGANKWWNLMPTHGTLPNHSLPGVPGPHAAGGVLRTTVQQSRKALPPGTITDLRL*

                                

Nucleotide sequence: 4293 bp .

                                    >EFF01499 HG326223:1689650-1693942 Type VI DNase and RNase Effector (Tde) [Serratia marcescens subsp. marcescens Db11]
ATGACCGAAGCGGCCCGCGTCGGCGATACCATCGGGCATTCCCATGCCCTGGCCGGCATGATTGCCGGCACCCTTGTCGG
CGGCCTGATCGCCGCCGCAGGCGCACTGGCGGCGGGTGCGCTGTTCGTCGCCGGCCTGGCGGCTTCCTGTATCGGCGTCG
GCGTGCTGCTTATCGGCGCCAGCCTGGCGGTGGGGTATCTCACCGGGGAGGCGGCCACCGCGGCGCGCGACGGCATTGCC
GACGCCGGTGCCGGTAGCTTGACCCCCAAAGGCAATATCGTCACCGGCTCCCCCAACGTCTTCATCAACGGCAAACCCGC
CGCGCTCGCCACCAACAGCCAGGTGGCCTGCAGCGACGACGGCCCGAGCATGCAGATGGCGCAGGGCTCCGACAAGGTCA
GCATCAACGGCCAGCCCGCCTCGCGTGTGGGGGACAAAACCAACTGCGACGCGCAGGTGATGGAAGGTTCGCCCAACGTG
TTTATCGGCGGCGGCACCGTCACCACCTTGCCGATCAAGCCCGAAGTGCCGGATTGGCTGTATAAGGTCTCCGATCTGAC
GCTGCTGTTCGCCGGCCTGGTGGGCGGCGTGGGCGGCGCCGCCGGCAAGCTGGGGGCGCTCGGCAAACTGCTGGGCAAGC
TGCCCGGCATCAACAAACTGGCGCGCATCGCCTGCCGTGCCGGCACCCTGATGACCGCGACCGCCGCGGTTGGCATTATC
GCCCGGCCGGTGGATATCGTCAGCGGCCAGAAATTCCTCGACGGCGAAGACGATCTCGATTTCGTGCTGCCATCGCGCCT
GCCGGTCGCCTGGCAGCGCTATTGGCGCAGCGGCAACCCCGGCGACAGCGTACTGGGCCGCGGCTGGAACCTGTTCTGGG
AGAGCAGCCTGCAGCCGTATCAGGACGGCCTGGTGTGGCGCGCGCCTTCCGGCGACTTCGTCGCTTTCCCCATGGTGCCG
CGCGGTCACAAAACTTACTGCGAAGCCGAAAAATGCTGGCTGATGCACAACGACGACGGCAGTTGGCAGCTGTTTGACGT
CGGCGAACAGATTTTCCACTACCCGCCGCTGGCGGGCGAGCAGCCGAGCCGGCTCAGCATGATCACCGACGCCATCGGCA
ACGCCACCTCGCTGTTTTACGACGACGAGGGGCTGCTGAGCGAACTGGTGGACAGCGCCGGGCAGCGCCTGATGTGCCGC
TATGCGCAGGGCCGCCTGCGCGAAGTGGCGCTGCAAACCGCCGAAGGCGAACGAACGCTGGCGCGCTACGGTTATGATGA
GCAGGGCCAACTGACGACGGTGAGCAACCGCGCCGGTGAGGTGACGCGCCGCTTTGGCTGGCGCGACGGCCTGATGATCA
GCCACCAGGACGCCGCCGGGCTGCTGAACGAATACCAATGGCAAGAGATCGACGGCGTGCCGCGCGTGACGGCCTATCGC
AACAGCGCCGGAGAATCCCTTGAGTTCGGCTATGACTTCGCCGGCGGGCGCCGCAGCGCGGTGCGCGGCGACGGCAAGCG
CGCGGAGTGGCGGCTGGATGACGACGACAACGTGGCGCAGTACACCGATTTCGACCAGCGCCGCTACGGTTTTATCTACC
AACGCGGCGAGCTGTGCAGCGTGCTGCTGCCCGGCGGCGCGCAGCGCCAGAGCGAATGGGACCCTTACGGCCGCCTGTTG
TCGGAGACCGACCCGCTCGGCCGCGTGACGCGCTATCAATATTCGCGCAACAGCGGCCGGCTGTTCGCCGTCGCGTACCC
GGACGGCAGCAGCGAGGCGCAGCACTGGGACACGCTGGGGCGCCCGACGCGTTATGTCGATGCGCTGGGCAATACCACGC
TGTACCGCTATCCGGATGACGAAGAGAGCCTGCCGGCTAGTGTGATCGACGCGCTGGGCGGGGAAGTGAAGCTGGAGTGG
GACGCCCGCGGCCAGCTGACGCGCTACACCGACTGTTCCGGCAGCGTCACGGCTTATACCTACGATGCGCTGGGGCAACT
GACGGCGCAGACCGATGCCGAAGGCCACCAGACGCGCTACCGGTGGGATAACGGCGGGCGCCTGCACACCCTGATCCACC
CGGACGGCGGCGAAGAGCGTTTTAACTGGAATGCGCACGGCCAGCTGGCCGAACATCAGGACGCGCTGGGCAGCCTGACC
CGCTGGCAGTACAACGCCCTGGGGCTGCCGGTCAGCATCACCGATCGCATCAACCGCACCCGGCGCTATCACTACAGCCC
GCAGGGCTGGCTGACGCGGCTGGAGAACGGCAACGGCGGCGACTACCGCTTCAGCTACGATGCGGTGGGCCGTGTGGTGG
CCGAAGAGCGCCCGGACGACACCCGCCACTATTATCGTTACGGCGCGGCCGGGCTGCTGGAAGAGCACCGTGAGGTCGGC
CTGCCGGGCGGCGCGGGCGAGCTGACGCAGCGCGAACAGCGCTTCCGCTTCGACGAGGCCGGGCAGCTGGTCTGGCGCAG
TAACGCCAGTGCGGAATGGCATTACCGTTTCGACGCCATGGGGCGGCTGCGTGAGCTGAACCGCCTGCCGACGGCGAGCG
GCGTGGCGCTGGGCATCGAGCCGGACAGCGTGCAGATGCGCTATGACGCCGCCGGGCGGCTGCTCGGCGAGCAAGGCGTC
AACGGCGAGCTGCAATACCAATGGGATGCATTGGCCAACCTGCAGGCGCTGACGCTGCCGCAGGGCGATCGCCTGCAGTG
GCTGTATTACGGCTCCGGCCACGCCAGCGCCATCAAATTCAACCAGCAGGTGGTGAGCGAGTTCACCCGCGATCGCCTGC
ACCGGGAAACCGGCCGCAGTCAGGGCGCGCTGCAGCAGCAGCGGCGTTACGACGCCATGGGCCGCCGCAGCTGGCAGAGC
AGCGCCTTCGGCCACGACAAACTGACCCGGCCGGAAGACGGCGTGCTGTGGCGCGCTTATCGCTACACCGGCCGCGGCGA
GCTGGCCGGGGTGAGCGACGCGCTGCGCGGCGAAGTGCACTACGGTTACGACGCCGAGGGCCGCCTGCTGCAACACCGCG
AACCCAATCAGGGCAAACCGGGCGCGCGGCTGGTGTACGACCTGGCGGATAACCTGCTGGGCGAGCGCAGCCCGCAGAGC
GATATCGACGCGCACCTGCCGCTGGCGCCGATCGCCGACAACCGCCTGACGCACTGGCAAAAACTGTTTTACCGTTACGA
TGCCTGGGGCAACCTGATCAGCCGGCGCAACGGTCTGTACGAACAGCACTACCGCTACGACGCCGACAACCGGCTGGTGC
AGGCGCACGGCCGCGGCCCGCAGGGCGAGTTCGAGGCGCAGTACCATTACGACGCGCTGGGACGCCGCAGCCGCAAAACG
GTGCACTACAAGGGCAAAACCGAACAGACGACCCATTTCCTGTGGCAGGGTTATCGGTTGCTGCAGGAGCAGCGCGACGA
CGGCAGCCGCCGCAGCTGGAGCTACGATCCGGCCAGCCCGTGGAGCCCGTTGGCGGCGCTGGAGCAGGCAGGCGACAGCC
GCTCGGCGGATATTTACTGGTATCACACCGATCTGAACAGCGCGCCGCTGGAGGTGACCGACGCGGCGGGCAACCTGTGC
TGGTCCGGGCAATACGACACCTTCGGCAAGCTGCAGGGCCAGACGGTGGCCGGCGCGGCGAAGCGGCAGGGCGCGCAATA
CCAGCAGCCGCTGCGCTACGCCGGGCAATACCAGGATGACGAAAGTGGCCTGCACTACAACCTGTTCCGCTACTACGAAC
CCGAGGTGGGGCGTTTCACCACGCAGGATCCGATAGGGTTGGAAGGCGGGCTGAATTTATATGCTTATGGGCCAAATCCT
TTAACTTGGATAGATCCATTTGGGTTAAGTAATTGTTCGACTCTTGACCGTATTATTGGTGATGCGAACAAAGTCGCTTC
GCGTGGTGGAGCTATAACAGCAAAACAAGCTCAGATACTGAGAGATAATTTACCGGTAGTTCAGAGACGGAGTGTTTTCC
AAAATCAGATGGCTCGCAAAGAGTTCGTCAGAGATCAGCATTATCTGATGAGTCAGTGGGAAGCCAATACAGGTAGAACC
TGGCCGACAGGAGCTACCCCGCACCACATAATCCCACTAGAAAGTGGGGGGGCGAATAAATGGTGGAATTTGATGCCTAC
TCATGGCACTTTGCCTAATCATTCTTTACCTGGTGTACCTGGCCCACATGCCGCCGGTGGAGTGCTTCGAACAACTGTTC
AACAAAGCAGAAAAGCACTGCCTCCTGGAACTATAACTGATTTGAGATTGTGA

                                