Detailed information of accessory protein


Summary

Accessory protein ID ACP00029 experimental
Type chaperone
Related T6SS (Type) T6SS00929 (Type i3)
Strain Serratia marcescens subsp. marcescens Db11
Replicon chromosome
Related structure protein SMDB11_2255 (TssM); SMDB11_2251; SMDB11_2269
Related effector SMDB11_3980 (Ssp4); SMDB11_2264 (Ssp2)
Description The presence of DsbA in target cells is essential for the activation of incoming periplasmic-acting effectors Ssp2 and Ssp4, and is required in the secreting cell for full T6SS function and acts on several components of the secretion machinery
Reference [1] Mariano G, Monlezun L, Coulthurst SJ. Dual Role for DsbA in Attacking and Targeted Bacterial Cells during Type VI Secretion System-Mediated Competition. Cell Rep. 2018 Jan 16;22(3):774-785. doi: 10.1016/j.celrep.2017.12.075. PMID: 29346773
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) SMDB11_0239 (dsbA2)
Coordinates (Strand) 265001..265642 (+)
NCBI ID 560172104
GenBank HG326223
Uniprot ID UPI0003C9AE1C
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DSBA PF01323.22 4.2e-19 46..200
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-213 MLAKVKRSFAAAVVLMLALPAVQAADYRAGEQYTRLDKPVAAAPAVVEFFSFYCGPCYQFAETYRVGSTVAQALPAGEKVTKYHVSLMGKLGNELTEAWAVATVLGVEDKIEGAMFDAVQKQRAVNGADDIQRVFTAAGIDAATYENARHSLLVKGLIAKQNEAVKAFEVRGTPSFYVAGKYKIDNAGMASTSVEGYAKEYAAVVRYLLDKQP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9968 pos: 24-25. VQA-AD. MLAKVKRSFAAAVVLMLALPAVQA


  Protein sequence: 214 a.a.    

>ACP00029 HG326223:265001-265642 [Serratia marcescens subsp. marcescens Db11]
MLAKVKRSFAAAVVLMLALPAVQAADYRAGEQYTRLDKPVAAAPAVVEFFSFYCGPCYQFAETYRVGSTVAQALPAGEKV
TKYHVSLMGKLGNELTEAWAVATVLGVEDKIEGAMFDAVQKQRAVNGADDIQRVFTAAGIDAATYENARHSLLVKGLIAK
QNEAVKAFEVRGTPSFYVAGKYKIDNAGMASTSVEGYAKEYAAVVRYLLDKQP*

  Nucleotide sequence: 642 bp    

>ACP00029 HG326223:265001-265642 [Serratia marcescens subsp. marcescens Db11]
ATGTTGGCAAAAGTTAAGCGTTCGTTCGCCGCCGCCGTCGTATTGATGTTGGCGCTGCCTGCGGTTCAGGCCGCGGATTA
CCGTGCGGGCGAGCAATACACCCGGCTGGATAAGCCGGTGGCCGCCGCGCCGGCGGTGGTGGAGTTCTTCTCCTTCTACT
GCGGCCCTTGCTATCAGTTCGCCGAGACCTATCGCGTAGGCAGTACCGTGGCGCAGGCGCTGCCCGCCGGTGAGAAAGTC
ACCAAATATCACGTCAGCCTGATGGGCAAACTGGGCAATGAGCTGACCGAAGCCTGGGCGGTGGCGACGGTGCTGGGCGT
GGAAGACAAGATTGAAGGGGCGATGTTCGACGCGGTGCAGAAACAGCGCGCCGTCAACGGCGCCGACGATATTCAGCGGG
TGTTTACGGCGGCCGGCATCGATGCCGCTACCTATGAAAACGCGCGCCACAGCCTGCTGGTCAAAGGATTGATCGCCAAG
CAGAACGAAGCGGTGAAAGCATTCGAGGTGCGCGGCACCCCGTCGTTCTACGTGGCAGGCAAATACAAGATAGACAATGC
CGGCATGGCCAGCACCAGCGTTGAAGGCTACGCCAAAGAGTACGCCGCGGTGGTGCGTTACCTGTTGGACAAACAGCCGT
AA