Detailed information of regulator
Summary
| Regulator ID | REG0016  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00929 (Type i3) |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon | chromosome |
| Sequence | Protein sequence (329 a.a.); Nucleotide sequence (987 bp) |
| Description | RtkS is an upstream activator of PpkA which stabilises the kinase through a direct interaction. |
| Reference |
External database links
| Locus tag (Gene) | SMDB11_2269 (RtkS) |
| Coordinates (Strand) | 2391034..2392020 (+) |
| NCBI ID | 560174112 |
| GenBank | HG326223 |
| Uniprot ID | UPI000321F8FA |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| SMDB11_2275 (PpKA) | ↓ | 560174118 |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-328 | MKYWMPGLVALMAVPTAQAMNYRLVYSPSQKLEVFIDDVKNSNPASWCGKSIPLRIVSAQSQDPAVLNDFLPRVGNLLEKQCPKAGHLPWTLTDKRGEKLASGEAAKARGWKPVVKQEAVEQPETPPQPAIPAAVAVTSPQADTAPPQRFSLPQGCHFRTFWGDEANGGALFIPAGEALRCGEDGWLQGSGAVTLQQGGQTLSPTLWFLQGYPLAKVNGGDRALTVVSANAQRLVLGGNPQAPGSFLLLSFEPKLHAWAFNGEAIVEMPRVDAADENKVKQHVQQAQTAWQPLLSAPAPLTFKLVEKLAADRVDPASGSYLSVNGATH |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9963 | pos: 19-20. AQA-MN. | MKYWMPGLVALMAVPTAQA |
Protein sequence of regulator: 329 a.a. .
>REG0016 HG326223:2391034-2392020 [Serratia marcescens subsp. marcescens Db11]
MKYWMPGLVALMAVPTAQAMNYRLVYSPSQKLEVFIDDVKNSNPASWCGKSIPLRIVSAQSQDPAVLNDFLPRVGNLLEK
QCPKAGHLPWTLTDKRGEKLASGEAAKARGWKPVVKQEAVEQPETPPQPAIPAAVAVTSPQADTAPPQRFSLPQGCHFRT
FWGDEANGGALFIPAGEALRCGEDGWLQGSGAVTLQQGGQTLSPTLWFLQGYPLAKVNGGDRALTVVSANAQRLVLGGNP
QAPGSFLLLSFEPKLHAWAFNGEAIVEMPRVDAADENKVKQHVQQAQTAWQPLLSAPAPLTFKLVEKLAADRVDPASGSY
LSVNGATH*
MKYWMPGLVALMAVPTAQAMNYRLVYSPSQKLEVFIDDVKNSNPASWCGKSIPLRIVSAQSQDPAVLNDFLPRVGNLLEK
QCPKAGHLPWTLTDKRGEKLASGEAAKARGWKPVVKQEAVEQPETPPQPAIPAAVAVTSPQADTAPPQRFSLPQGCHFRT
FWGDEANGGALFIPAGEALRCGEDGWLQGSGAVTLQQGGQTLSPTLWFLQGYPLAKVNGGDRALTVVSANAQRLVLGGNP
QAPGSFLLLSFEPKLHAWAFNGEAIVEMPRVDAADENKVKQHVQQAQTAWQPLLSAPAPLTFKLVEKLAADRVDPASGSY
LSVNGATH*
Nucleotide sequence of regulator: 987 bp .
>REG0016 HG326223:2391034-2392020 [Serratia marcescens subsp. marcescens Db11]
ATGAAATATTGGATGCCGGGGCTCGTCGCCCTGATGGCGGTGCCGACCGCGCAGGCGATGAACTATCGCCTGGTTTACTC
CCCCAGCCAGAAGCTGGAAGTGTTTATCGATGACGTCAAAAACAGCAATCCGGCAAGCTGGTGCGGCAAGAGCATCCCGC
TGCGCATCGTCTCGGCGCAAAGCCAGGATCCGGCGGTGCTGAACGACTTCCTGCCGCGCGTCGGCAATCTGTTGGAAAAG
CAGTGCCCGAAAGCCGGCCACCTGCCCTGGACGCTGACCGACAAGCGCGGTGAGAAGCTGGCCAGCGGCGAAGCCGCCAA
AGCGCGCGGCTGGAAACCGGTGGTCAAACAGGAGGCCGTCGAGCAGCCGGAAACCCCGCCGCAGCCGGCTATCCCTGCTG
CAGTGGCCGTGACTTCTCCGCAGGCGGACACCGCGCCGCCTCAGCGTTTCAGCCTGCCGCAGGGCTGCCACTTCCGCACC
TTCTGGGGTGACGAAGCCAACGGCGGCGCGCTATTCATTCCCGCTGGTGAGGCGCTGCGCTGCGGCGAGGACGGCTGGCT
GCAGGGCAGCGGCGCGGTCACGCTGCAACAGGGCGGCCAAACCCTCTCGCCGACGCTGTGGTTCCTGCAAGGTTACCCGC
TGGCGAAGGTGAACGGCGGCGATCGCGCGCTGACCGTCGTCAGCGCCAACGCCCAACGGCTGGTTTTGGGCGGCAACCCG
CAGGCGCCGGGCAGCTTCCTGCTGCTGAGCTTCGAACCGAAGCTGCACGCCTGGGCGTTCAACGGTGAAGCGATCGTTGA
AATGCCGCGCGTCGATGCCGCCGACGAGAACAAGGTCAAACAGCACGTACAGCAGGCGCAAACCGCCTGGCAGCCGCTGC
TAAGCGCCCCCGCCCCGCTGACCTTCAAGCTGGTGGAAAAGCTGGCCGCAGACCGCGTCGATCCTGCCAGCGGCAGCTAC
CTGTCGGTTAACGGCGCCACTCACTGA
ATGAAATATTGGATGCCGGGGCTCGTCGCCCTGATGGCGGTGCCGACCGCGCAGGCGATGAACTATCGCCTGGTTTACTC
CCCCAGCCAGAAGCTGGAAGTGTTTATCGATGACGTCAAAAACAGCAATCCGGCAAGCTGGTGCGGCAAGAGCATCCCGC
TGCGCATCGTCTCGGCGCAAAGCCAGGATCCGGCGGTGCTGAACGACTTCCTGCCGCGCGTCGGCAATCTGTTGGAAAAG
CAGTGCCCGAAAGCCGGCCACCTGCCCTGGACGCTGACCGACAAGCGCGGTGAGAAGCTGGCCAGCGGCGAAGCCGCCAA
AGCGCGCGGCTGGAAACCGGTGGTCAAACAGGAGGCCGTCGAGCAGCCGGAAACCCCGCCGCAGCCGGCTATCCCTGCTG
CAGTGGCCGTGACTTCTCCGCAGGCGGACACCGCGCCGCCTCAGCGTTTCAGCCTGCCGCAGGGCTGCCACTTCCGCACC
TTCTGGGGTGACGAAGCCAACGGCGGCGCGCTATTCATTCCCGCTGGTGAGGCGCTGCGCTGCGGCGAGGACGGCTGGCT
GCAGGGCAGCGGCGCGGTCACGCTGCAACAGGGCGGCCAAACCCTCTCGCCGACGCTGTGGTTCCTGCAAGGTTACCCGC
TGGCGAAGGTGAACGGCGGCGATCGCGCGCTGACCGTCGTCAGCGCCAACGCCCAACGGCTGGTTTTGGGCGGCAACCCG
CAGGCGCCGGGCAGCTTCCTGCTGCTGAGCTTCGAACCGAAGCTGCACGCCTGGGCGTTCAACGGTGAAGCGATCGTTGA
AATGCCGCGCGTCGATGCCGCCGACGAGAACAAGGTCAAACAGCACGTACAGCAGGCGCAAACCGCCTGGCAGCCGCTGC
TAAGCGCCCCCGCCCCGCTGACCTTCAAGCTGGTGGAAAAGCTGGCCGCAGACCGCGTCGATCCTGCCAGCGGCAGCTAC
CTGTCGGTTAACGGCGCCACTCACTGA