Detailed information of accessory protein


Summary

Accessory protein ID ACP00028 experimental
Type chaperone
Related T6SS (Type) T6SS00929 (Type i3)
Strain Serratia marcescens subsp. marcescens Db11
Replicon chromosome
Related structure protein SMDB11_2255 (TssM); SMDB11_2251; SMDB11_2269
Related effector SMDB11_3980 (Ssp4); SMDB11_2264 (Ssp2)
Description The presence of DsbA in target cells is essential for the activation of incoming periplasmic-acting effectors Ssp2 and Ssp4, and is required in the secreting cell for full T6SS function and acts on several components of the secretion machinery
Reference [1] Mariano G, Monlezun L, Coulthurst SJ. Dual Role for DsbA in Attacking and Targeted Bacterial Cells during Type VI Secretion System-Mediated Competition. Cell Rep. 2018 Jan 16;22(3):774-785. doi: 10.1016/j.celrep.2017.12.075. PMID: 29346773
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) SMDB11_4101 (dsbA1)
Coordinates (Strand) 4377737..4378360 (-)
NCBI ID 560175924
GenBank HG326223
Uniprot ID UPI0003254861
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DSBA PF01323.22 8.6e-29 40..186
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-207 MKKIWLALVGMVMAFSASAAQFSDGTQYVTLDKPVTGEPQVLEFFSFYCPHCYQFEQVYHVSENVKKALPAGTKMTKYHVEFLGPLGKQLTQAWAVAMALGVEDKVSPLMFEAVQKTQTVQTPDDIRNVFVKAGVTAADYDAAWNSFVVKSLVVQQEKAAEDLQLRGVPAVFVNGKYMVKNDGLDTSSMDAYVKQFADVVKFLSQQK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9976 pos: 19-20. ASA-AQ. MKKIWLALVGMVMAFSASA


  Protein sequence: 208 a.a.    

>ACP00028 HG326223:c4378360-4377737 [Serratia marcescens subsp. marcescens Db11]
MKKIWLALVGMVMAFSASAAQFSDGTQYVTLDKPVTGEPQVLEFFSFYCPHCYQFEQVYHVSENVKKALPAGTKMTKYHV
EFLGPLGKQLTQAWAVAMALGVEDKVSPLMFEAVQKTQTVQTPDDIRNVFVKAGVTAADYDAAWNSFVVKSLVVQQEKAA
EDLQLRGVPAVFVNGKYMVKNDGLDTSSMDAYVKQFADVVKFLSQQK*

  Nucleotide sequence: 624 bp    

>ACP00028 HG326223:c4378360-4377737 [Serratia marcescens subsp. marcescens Db11]
ATGAAAAAAATATGGTTGGCGCTCGTTGGCATGGTGATGGCATTCAGTGCCTCGGCGGCACAGTTTAGCGATGGCACTCA
GTATGTGACCTTGGACAAGCCGGTGACCGGCGAGCCGCAGGTACTGGAGTTCTTCTCCTTCTACTGCCCGCACTGCTACC
AATTTGAGCAGGTCTATCACGTGTCCGAAAACGTGAAAAAAGCGCTGCCTGCCGGCACCAAAATGACCAAGTACCACGTG
GAGTTCCTGGGGCCACTGGGCAAACAGCTGACTCAGGCGTGGGCGGTGGCGATGGCGCTGGGCGTGGAAGATAAAGTAAG
CCCGCTGATGTTTGAAGCGGTGCAGAAAACCCAGACCGTGCAAACGCCGGACGATATCCGTAACGTCTTCGTAAAAGCGG
GCGTGACCGCCGCAGATTATGACGCAGCTTGGAACAGCTTCGTGGTGAAATCTCTGGTGGTTCAACAGGAGAAAGCCGCG
GAAGATCTGCAGCTGCGCGGCGTGCCGGCGGTATTTGTGAACGGCAAATACATGGTTAAAAACGATGGCCTGGATACCAG
CTCGATGGACGCTTACGTGAAGCAGTTCGCCGACGTGGTTAAATTCCTCAGCCAGCAGAAATAA