Detailed information of regulator


Summary

Regulator ID REG0043 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) Bacterial secretion system (map03070); Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (1033 a.a.); Nucleotide sequence (3099 bp)
Description When the appropriate environmental signal is sensed, PpkA becomes activated and autophosphorylates, leading to Fha1 binding. Fha1 binding promotes its phosphorylation and p-Fha1 levels sufficient to trigger H-T6S are reached.
Reference
External database links

Locus tag (Gene) PA0074 (PpkA)
Coordinates (Strand) 87000..90098 (-)
NCBI ID 15595272
RefSeq NC_002516
Uniprot ID Q9I758_PSEAE
KEGG ID pae:PA0074
KEGG pathway (determined by BlastKOALA) map03070; map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
PA0081 (Fha-1/TagH) ↓ 15595279
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Pkinase PF00069.27 4.2e-50 9..250
VWA PF00092.30 1.6e-05 616..780
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1032 MDIELPGFDIERELGEGAMATVYLATQRSLQRKVALKVMAAALAADPSFAERFLREGRTLARLSHPNTVTIHDIGNVGSCYYMAMEYLPNGTLKERIQQGLDPEQGLAYVRQVAAALGYAHSQGLVHRDVKPANILFRADGTAVLSDFGIAKSIEDNTQFTQVGFAVGTPSYMSPEQARGQEIDGRADLYALGVVLYEILTGKLPYNGKDSLSTALAHLTEPLPELPIEQGRYQDILRQLLAKDPAERFPTAGALIAALDRLAPSALEATQIRPLATPQGSPRASNPPPAEPAPLPPADLGGLQPVSIQLPPVTPSAGGATPPPPPPSQAAKPPSPPPPPLPPAKPRAGGSRTPLIAAAAAAVLLAIGGGAWWLLGSGDAPSAADPAPLGQTPADKPQQQTPVADAEQDGGERPLLMPGKKTLFQRVLTKPGAAISAEAGGPPRAGAVPAFSVLYVYARKQVGGSPWLRVGASSNGEGDGWLPASAVSDWKQSLVLKFTERSGRAPVMFLNSAEQVERLLGDTRAARDTLTQAVDHKGDPQVVAVEPNDRAIPQDQFYLLPIFDSKESFDADGQPAQLLEVASIDPGGSAAAPQAPGQAGQGGASGAASDTFRTGIVLVVDTSVSMQPYIDRVREVIDGLQRQIGERGDLDKVSFGLVGFRNNTDKTPGLEYVSKTLVPLQPGNDAQRFAELSSQVRATDVSSHSFNEDAFAGIMQAVDEMDWSPYAGRVVLLVTDAGALRKNDPLGRTQMNEAEVRQAALRKGVKIYALHLRTPAGKNNHGYAEQQYRSLTADANPKIADLYIPVAGGEVNAFGNTVKEIGTVFADLVHDAGNRKPQAPRFDAAPSVASKSAAIGYAMQMEFLGRRDPVRAPQVVTAWTADRDLTNPALPAFQVCVLLSKLQLNELQQSLKLIVDAAKRTQTSPKDFFQEIASASAYMSRDPAQLVKGSNLAQSGVLGEYLEGLPYRSKSLNMTQDLWLSLSVAEQQDFIDELESKIRLYETFHNDVANWVRFGDADAGDALYRVPLSTLP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.4984 - -


  Protein sequence of regulator: 1033 a.a.    .

>REG0043 NC_002516:c90098-87000 [Pseudomonas aeruginosa PAO1]
MDIELPGFDIERELGEGAMATVYLATQRSLQRKVALKVMAAALAADPSFAERFLREGRTLARLSHPNTVTIHDIGNVGSC
YYMAMEYLPNGTLKERIQQGLDPEQGLAYVRQVAAALGYAHSQGLVHRDVKPANILFRADGTAVLSDFGIAKSIEDNTQF
TQVGFAVGTPSYMSPEQARGQEIDGRADLYALGVVLYEILTGKLPYNGKDSLSTALAHLTEPLPELPIEQGRYQDILRQL
LAKDPAERFPTAGALIAALDRLAPSALEATQIRPLATPQGSPRASNPPPAEPAPLPPADLGGLQPVSIQLPPVTPSAGGA
TPPPPPPSQAAKPPSPPPPPLPPAKPRAGGSRTPLIAAAAAAVLLAIGGGAWWLLGSGDAPSAADPAPLGQTPADKPQQQ
TPVADAEQDGGERPLLMPGKKTLFQRVLTKPGAAISAEAGGPPRAGAVPAFSVLYVYARKQVGGSPWLRVGASSNGEGDG
WLPASAVSDWKQSLVLKFTERSGRAPVMFLNSAEQVERLLGDTRAARDTLTQAVDHKGDPQVVAVEPNDRAIPQDQFYLL
PIFDSKESFDADGQPAQLLEVASIDPGGSAAAPQAPGQAGQGGASGAASDTFRTGIVLVVDTSVSMQPYIDRVREVIDGL
QRQIGERGDLDKVSFGLVGFRNNTDKTPGLEYVSKTLVPLQPGNDAQRFAELSSQVRATDVSSHSFNEDAFAGIMQAVDE
MDWSPYAGRVVLLVTDAGALRKNDPLGRTQMNEAEVRQAALRKGVKIYALHLRTPAGKNNHGYAEQQYRSLTADANPKIA
DLYIPVAGGEVNAFGNTVKEIGTVFADLVHDAGNRKPQAPRFDAAPSVASKSAAIGYAMQMEFLGRRDPVRAPQVVTAWT
ADRDLTNPALPAFQVCVLLSKLQLNELQQSLKLIVDAAKRTQTSPKDFFQEIASASAYMSRDPAQLVKGSNLAQSGVLGE
YLEGLPYRSKSLNMTQDLWLSLSVAEQQDFIDELESKIRLYETFHNDVANWVRFGDADAGDALYRVPLSTLP*

  Nucleotide sequence of regulator: 3099 bp    .

>REG0043 NC_002516:c90098-87000 [Pseudomonas aeruginosa PAO1]
ATGGACATAGAACTTCCGGGATTCGACATCGAACGCGAGCTGGGCGAAGGCGCCATGGCCACGGTCTACCTGGCCACCCA
GCGCTCGCTGCAGCGCAAGGTGGCGCTGAAGGTGATGGCCGCGGCGCTGGCCGCCGACCCGAGCTTCGCCGAACGCTTCC
TGCGCGAAGGTCGGACCCTGGCGCGGCTGTCGCACCCGAACACGGTGACCATCCACGACATCGGCAACGTCGGCAGTTGC
TACTACATGGCCATGGAATACCTGCCCAACGGCACGCTCAAGGAGCGCATCCAGCAGGGCCTCGATCCCGAGCAGGGCCT
GGCCTACGTACGGCAGGTGGCCGCCGCGCTCGGCTACGCGCACTCCCAGGGCCTGGTGCATCGCGACGTGAAGCCGGCCA
ACATCCTGTTCCGCGCCGACGGCACCGCGGTGCTCTCGGACTTCGGCATCGCCAAGTCGATCGAGGACAATACCCAGTTC
ACCCAGGTCGGCTTCGCCGTCGGCACCCCCAGCTACATGAGCCCGGAGCAGGCCCGCGGGCAGGAGATCGACGGCCGCGC
CGACCTCTACGCGCTGGGCGTGGTGCTCTACGAGATCCTCACCGGCAAGCTGCCCTACAACGGCAAGGACTCGCTCTCCA
CCGCCCTGGCCCACCTCACCGAGCCGCTGCCGGAACTGCCGATCGAGCAGGGCCGCTACCAGGACATCCTGCGCCAGTTG
CTGGCCAAGGACCCGGCCGAGCGCTTCCCCACCGCCGGCGCGTTGATCGCCGCCCTCGACCGCCTGGCGCCGAGCGCCCT
GGAAGCCACCCAGATCCGTCCGCTGGCGACGCCGCAGGGCTCGCCGCGAGCGAGCAACCCGCCGCCGGCCGAACCCGCGC
CCCTGCCGCCCGCCGACCTCGGCGGCCTGCAACCGGTGTCGATCCAGTTGCCGCCGGTCACGCCCAGCGCCGGCGGCGCC
ACGCCGCCTCCGCCTCCGCCGTCGCAAGCGGCGAAACCGCCATCGCCCCCGCCGCCTCCGTTGCCGCCGGCGAAGCCACG
TGCCGGCGGCAGCCGCACGCCGTTGATCGCCGCCGCCGCGGCCGCCGTGCTCCTGGCGATCGGCGGCGGCGCCTGGTGGC
TGCTGGGCTCGGGCGACGCGCCGAGCGCTGCCGATCCAGCGCCGCTGGGACAGACGCCGGCGGACAAGCCGCAGCAGCAG
ACCCCGGTGGCCGATGCCGAACAGGACGGCGGCGAGCGTCCGCTGCTGATGCCAGGCAAGAAGACGCTGTTCCAGCGCGT
CCTGACCAAACCCGGCGCGGCCATCTCCGCCGAGGCTGGCGGCCCGCCCCGGGCCGGCGCGGTGCCGGCCTTCTCGGTGC
TCTACGTGTACGCCCGCAAGCAGGTCGGCGGCAGCCCCTGGCTGCGCGTCGGCGCCTCCAGCAACGGCGAGGGCGACGGC
TGGCTGCCGGCTAGCGCGGTGAGCGACTGGAAGCAGAGCCTGGTGCTGAAGTTCACCGAACGCTCCGGCCGCGCGCCGGT
GATGTTCCTCAACAGCGCCGAGCAGGTCGAGCGCCTGCTCGGCGATACCCGCGCAGCGCGCGACACCCTGACCCAGGCGG
TGGATCACAAGGGCGATCCGCAGGTGGTCGCGGTCGAGCCCAACGACCGGGCGATCCCCCAGGACCAGTTCTACCTGCTG
CCGATCTTCGACTCGAAGGAAAGCTTCGACGCCGACGGCCAGCCGGCGCAGTTGCTGGAGGTCGCCTCGATCGACCCCGG
CGGCAGCGCCGCGGCACCCCAGGCGCCGGGCCAGGCCGGGCAGGGCGGCGCCAGCGGCGCGGCGAGCGACACCTTCCGCA
CCGGCATCGTCCTGGTGGTGGACACCTCGGTGTCGATGCAGCCCTACATCGACCGCGTGCGCGAGGTGATCGACGGCCTG
CAGCGGCAGATCGGCGAGCGCGGCGACCTGGACAAGGTCAGCTTCGGCCTGGTCGGTTTCCGCAACAACACCGACAAGAC
CCCCGGCCTGGAATACGTCAGCAAGACCCTGGTGCCGCTGCAACCGGGCAACGACGCGCAGCGCTTCGCCGAGTTGAGCA
GCCAGGTGCGCGCCACCGACGTCTCCAGCCACTCGTTCAACGAAGACGCCTTCGCCGGGATCATGCAGGCGGTCGACGAA
ATGGACTGGTCGCCCTACGCCGGGCGCGTGGTGCTGCTGGTCACCGACGCCGGCGCGCTGCGCAAGAACGACCCGCTCGG
GCGCACCCAGATGAACGAGGCCGAGGTCCGCCAGGCGGCGCTGCGCAAGGGCGTGAAGATCTATGCCCTGCACCTGCGCA
CCCCGGCCGGGAAGAACAACCACGGCTACGCCGAGCAGCAGTACCGCAGCCTCACCGCCGACGCCAACCCGAAGATCGCC
GACCTCTACATCCCGGTGGCCGGCGGCGAGGTGAACGCCTTCGGCAACACGGTGAAGGAGATCGGCACGGTGTTCGCCGA
CCTGGTCCACGACGCCGGCAACCGCAAGCCGCAGGCGCCGCGCTTCGACGCCGCGCCAAGCGTGGCGAGCAAGTCGGCGG
CCATCGGCTACGCCATGCAGATGGAATTCCTCGGTCGCCGCGACCCGGTGCGCGCGCCGCAGGTCGTCACCGCCTGGACC
GCCGACCGTGACCTGACCAACCCGGCGCTGCCGGCGTTCCAGGTCTGCGTGCTGCTGAGCAAGCTGCAACTCAACGAACT
GCAACAGTCGCTGAAACTGATCGTCGACGCGGCCAAGCGCACCCAGACCTCGCCCAAGGACTTCTTCCAGGAAATCGCCA
GCGCCAGCGCCTACATGAGCCGCGACCCGGCGCAACTGGTGAAGGGCAGCAACCTGGCGCAGAGCGGCGTACTCGGCGAA
TACCTGGAAGGCCTGCCGTACCGCAGCAAGTCGCTGAACATGACCCAGGACCTCTGGCTGTCGCTGAGCGTCGCCGAACA
GCAGGACTTCATCGACGAACTGGAGTCGAAGATCCGCCTCTACGAAACCTTCCACAACGACGTCGCCAACTGGGTCCGGT
TCGGCGACGCCGACGCCGGCGACGCGCTGTACCGCGTGCCGCTGTCGACGCTGCCATGA