Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00003 experimental
T6SS type Type i3
Strain Pseudomonas aeruginosa PAO1
Replicon (RefSeq) chromosome (H1-T6SS) (NC_002516)
Location 83380..123495
Function anti-bacterial; anti-eukaryotic; effector translocation; virulence
Comment Virulence in rat model of chronic respiratory infection; Ability to outcompete a susceptible mutant of P. aeruginosa; ability to outcompete P. putida; growth inhibition of E. coli.
References
[1] Leroux M et al (2012) Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword. Proc Natl Acad Sci U S A. 109(48):19804-9. [PudMed:23150540]
[2] Lossi NS et al (2012) The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system. Mol Microbiol. 86(2):437-56. [PudMed:22906320]
[3] Chou S et al (2012) Structure of a Peptidoglycan Amidase Effector Targeted to Gram-Negative Bacteria by the Type VI Secretion System. Cell Rep. 1(6):656-64. [PudMed:22813741]
[4] Casabona MG et al (2012) An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa. Environ Microbiol. 15(2):471-86. [PudMed:22765374]
[5] Zhang H et al (2012) Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa. FEBS Lett. 586(19):3193-9. [PudMed:22750141]
[6] Ding J et al (2012) Structural Insights into the Pseudomonas aeruginosa Type VI Virulence Effector Tse1 Bacteriolysis and Self-protection Mechanisms. J Biol Chem. 287(32):26911-20. [PudMed:22700987]
[7] Kefala K et al (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 6):695-7. [PudMed:22684073]
[8] Sana TG et al (2012) The Second Type VI Secretion System of Pseudomonas aeruginosa Strain PAO1 Is Regulated by Quorum Sensing and Fur and Modulates Internalization in Epithelial Cells. J Biol Chem. 287(32):27095-105. [PudMed:22665491]
[9] Li M et al (2012) Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathog. 8(4):e1002613. [PudMed:22511866]
[10] Zou T et al (2012) Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin. J Mol Biol. 417(4):351-61. [PudMed:22310046]
[11] Silverman JM et al (2011) Separate inputs modulate phosphorylation-dependent and -independent type VI secretion activation. Mol Microbiol. 82(5):1277-90. [PudMed:22017253]
[12] Goldova J et al (2011) A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence. BMC Genomics. 0.803472222. [PudMed:21880152]
[13] Lossi NS et al (2011) Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa. Microbiology. 157(Pt 12):3292-305. [PudMed:21873404]
[14] Russell AB et al (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature. 475(7356):343-7. [PudMed:21776080]
[15] Osipiuk J et al (2011) Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
[16] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
[17] Hachani A et al (2011) Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275]
[18] Bleves S et al (2010) Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
[19] Mougous JD et al (2006) A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science. 312(5779):1526-30. [PudMed:16763151]
[20] Robb CS et al (2013) Structure of the T6SS lipoprotein TssJ1 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 6):607-10. [PudMed:23722835]
[21] Lu D et al (2013) Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 5):524-7. [PudMed:23695568]
[22] Basler M et al (2013) Tit-for-Tat: Type VI Secretion System Counterattack during Bacterial Cell-Cell Interactions. Cell. 152(4):884-94. [PudMed:23415234]
[23] Hood RD et al (2010) A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe. 7(1):25-37. [PudMed:20114026]
[24] Whitney JC et al (2014) Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 92(3):529-42. [PudMed:24589350]
[25] Hachani A et al (2014) The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869]
[26] Mougous JD et al (2007) Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa. Nat Cell Biol. 9(7):797-803. [PudMed:17558395]
[27] Hsu F et al (2009) TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa. Mol Microbiol. 72(5):1111-25. [PudMed:19400797]
[28] Forster A et al (2014) Coevolution of the ATPase ClpV, the Sheath Proteins TssB and TssC and the Accessory Protein TagJ/HsiE1 Distinguishes Type VI Secretion Classes. J Biol Chem. 289(47):33032-43. [PudMed:25305017]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHAPpkAPppATagFTagJTagQTagRTagSTagTTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number1111111111111111131111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
PA0067 (prlC) 78710..80755 + 2046 NP_248757.1 oligopeptidase A
PA0068 80752..81027 + 276 NP_248758.1 hypothetical protein
PA0069 81116..82174 + 1059 NP_248759.1 hypothetical protein
T6CP012414 TagQ PA0070 82404..83318 - 915 NP_248760.1 hypothetical protein
T6CP012415 TagR PA0071 83380..85092 - 1713 NP_248761.1 hypothetical protein
T6CP012416 TagS PA0072 85085..86284 - 1200 NP_248762.1 hypothetical protein
T6CP012417 TagT PA0073 86284..87003 - 720 NP_248763.1 ABC transporter ATP-binding protein
T6CP012418 PpkA PA0074 (ppkA) 87000..90098 - 3099 NP_248764.1 serine/threonine protein kinase PpkA
T6CP012419 PppA PA0075 (pppA) 90106..90834 - 729 NP_248765.1 serine/threonine phosphatase
T6CP012420 TagF PA0076 90844..91524 - 681 NP_248766.1 hypothetical protein
T6CP005094 TssM PA0077 (icmF1) 91521..94826 - 3306 NP_248767.1 type VI secretion protein IcmF
T6CP005095 TssL PA0078 95048..96397 - 1350 NP_248768.1 hypothetical protein
T6CP005096 TssK PA0079 96404..97738 - 1335 NP_248769.1 hypothetical protein
T6CP005097 TssJ PA0080 97754..98218 - 465 NP_248770.1 hypothetical protein
T6CP011390 FHA PA0081 (fha1) 98263..99756 - 1494 NP_248771.1 Fha domain-containing protein
T6CP005098 TssA PA0082 100124..101158 + 1035 NP_248772.1 hypothetical protein
T6CP005099 TssB PA0083 101247..101765 + 519 NP_248773.1 hypothetical protein
T6CP005100 TssC PA0084 101778..103274 + 1497 NP_248774.1 hypothetical protein
T6CP005101 TssD PA0085 (hcp1) 103350..103838 + 489 NP_248775.1 protein secretion apparatus assembly protein
T6CP012694 TagJ PA0086 104006..104851 + 846 NP_248776.1 hypothetical protein
T6CP005102 TssE PA0087 104853..105362 + 510 NP_248777.1 hypothetical protein
T6CP005103 TssF PA0088 105359..107218 + 1860 NP_248778.1 hypothetical protein
T6CP005104 TssG PA0089 107182..108228 + 1047 NP_248779.1 hypothetical protein
T6CP005105 TssH PA0090 (clpV1) 108221..110929 + 2709 NP_248780.1 secretion protein ClpV1
T6CP005106 TssI PA0091 (vgrG1) 110976..112907 + 1932 NP_248781.1 type VI secretion system protein VgrG
PA0092 113022..113306 - 285 NP_248782.1 hypothetical protein
PA0093 113303..114595 - 1293 NP_248783.1 hypothetical protein
PA0094 114611..115045 - 435 NP_248784.1 hypothetical protein
T6CP005107 TssI PA0095 115299..117524 + 2226 NP_248785.1 hypothetical protein
PA0096 117552..118001 + 450 NP_248786.1 hypothetical protein
PA0097 117931..119130 + 1200 NP_248787.1 hypothetical protein
PA0098 119127..120164 + 1038 NP_248788.1 3-oxoacyl-ACP synthase
PA0099 120164..121324 + 1161 NP_248789.1 hypothetical protein
PA0100 121346..122266 + 921 NP_248790.1 hypothetical protein
PA0101 122248..123495 + 1248 NP_248791.1 hypothetical protein
PA0102 123871..124599 + 729 NP_248792.1 carbonic anhydrase
PA0103 124810..126381 + 1572 NP_248793.1 sulfate transporter
PA0103a 126368..127006 - 639 YP_008719730.1 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0018 experimental PA4462 (RpoN) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4992870..4994363 (+) 15599658 PA1512 (hcpA) promotor (↓); PA5267 (hcpB) promotor (↓)
REG0040 experimental PA0592 (RsmA) protein RsmA regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 650575..651381 (-) 15595789 FHA (PA0081) (↑)
REG0041 experimental PA5183a (RsmN) protein RsmA regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5836470..5836685 (-) 554753081 FHA (PA0081) (↑); PA3487 (pldA) expression (↑)
REG0042 experimental PA0075 (PppA) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 90106..90834 (-) 15595273 PA0081 (Fha-1/TagH) (↑)
REG0043 experimental PA0074 (PpkA) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 87000..90098 (-) 15595272 PA0081 (Fha-1/TagH) (↓)
REG0044 experimental PA2586 (gacA) protein TCS-RR Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 2925788..2926432 (-) 15597782 hcp1 (PA0085) expression and secretion (↓)
REG0045 experimental PA0610 (prtN) protein TCS-HK Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 672777..673091 (-) 15595807 PA0611 (prtR) (↑)
REG0046 experimental PA0611 (prtR) protein TCS-RR Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 673191..673961 (-) 15595808 hcp1 (PA0085) expression and secretion (↑); PA2586 (gacA) (↑)
REG0047 experimental PA0072 (TagS) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 85085..86284 (-) 15595270 PA0073 (TagT) (↓)
REG0048 experimental PA0070 (TagQ) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 82404..83318 (-) 15595268 PA0071 (tagR) (↓)
REG0049 experimental PA0073 (TagT) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 86284..87003 (-) 15595271 PA0072 (TagS) (↓)
REG0050 experimental PA2492 (MexT) protein Unknown Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 2807469..2808512 (+) 15597688 T6SS transcripts (↑)
REG0051 experimental PA1006 protein Unknown Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1090606..1090857 (+) 15596203 T6SS transcripts (↓)
REG0052 experimental PA0071 (tagR) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 83380..85092 (-) 15595269 PA0074 (PpkA) (↓)
REG0053 experimental PA4856 (RetS) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5452150..5454978 (+) 15600049 hcp1 (PA0085) expression and secretion (↑)
REG0054 experimental PA3974 (LadS) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4453289..4455676 (-) 15599169 hcp1 (PA0085) expression and secretion (↓)
REG0055 experimental PA4109 (AmpR) protein Unknown Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4592990..4593880 (-) 15599304 T6SS transcripts (↑)
REG0056 experimental PA0076 (TagF) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 90844..91524 (-) 15595274 hcp1 (PA0086) secretion (↑)
REG0057 experimental PA0081 (Fha-1/TagH) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 98263..99756 (-) 15595279 Unknown (↓); clpV (↓)
REG0058 experimental PA2976 (RNase E) protein Unknown Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 3332881..3336054 (+) 15598172 PA0077 (icmF1) expression (↑)
REG0059 experimental PA4704.1 (prrF1) ncRNA QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5283960..5284110 (+) 4179187 T6SS transcripts (↓)
REG0060 experimental PA4704.2 (prrF2) ncRNA QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5284172..5284319 (+) 4179185 T6SS transcripts (↓)
REG0376 experimental PA3648 (bamA) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4085062..4087455 (-) NP_252338.1 ClpV-GFP foci accumulate; H1-T6SS transcript (↑)
REG0377 experimental PA0595 (lptD) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 653753..656527 (-) NP_249286.1 ClpV-GFP foci accumulate; H1-T6SS transcript (↑)
REG0378 experimental PA0972 (tolB) protein Tpp Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1056049..1057347 (+) NP_249663.1 ClpV-GFP foci accumulate; H1-T6SS transcript (↑)

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00002 experimental PA0094 (eagT6) adaptor Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 114611..115045 (-) 15595292 - PA0093 (Tse6)
ACP00003 experimental PA0086 (tagJ) structure affiliated protein Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 104006..104851 (+) 15595284 PA0082 (TssA) -
ACP00004 experimental PA0086 (tagJ) structure affiliated protein Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 104006..104851 (+) 15595284 PA0083 (HisB1/TssB1) -