Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
T6SS ID | T6SS00003 |
T6SS type | Type i3 |
Strain | Pseudomonas aeruginosa PAO1 |
Replicon (RefSeq) | chromosome (H1-T6SS) (NC_002516) |
Location | 83380..123495 |
Function | anti-bacterial; anti-eukaryotic; effector translocation; virulence |
Comment | Virulence in rat model of chronic respiratory infection; Ability to outcompete a susceptible mutant of P. aeruginosa; ability to outcompete P. putida; growth inhibition of E. coli. |
References | [1] Zou T et al (2012) Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin. J Mol Biol. 417(4):351-61. [PudMed:22310046] [2] Silverman JM et al (2011) Separate inputs modulate phosphorylation-dependent and -independent type VI secretion activation. Mol Microbiol. 82(5):1277-90. [PudMed:22017253] [3] Hachani A et al (2014) The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] [4] Osipiuk J et al (2011) Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004] [5] Hsu F et al (2009) TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa. Mol Microbiol. 72(5):1111-25. [PudMed:19400797] [6] Leroux M et al (2012) Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword. Proc Natl Acad Sci U S A. 109(48):19804-9. [PudMed:23150540] [7] Li M et al (2012) Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathog. 8(4):e1002613. [PudMed:22511866] [8] Mougous JD et al (2007) Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa. Nat Cell Biol. 9(7):797-803. [PudMed:17558395] [9] Kefala K et al (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 6):695-7. [PudMed:22684073] [10] Lossi NS et al (2012) The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system. Mol Microbiol. 86(2):437-56. [PudMed:22906320] [11] Sana TG et al (2012) The Second Type VI Secretion System of Pseudomonas aeruginosa Strain PAO1 Is Regulated by Quorum Sensing and Fur and Modulates Internalization in Epithelial Cells. J Biol Chem. 287(32):27095-105. [PudMed:22665491] [12] Lu D et al (2013) Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 5):524-7. [PudMed:23695568] [13] Ding J et al (2012) Structural Insights into the Pseudomonas aeruginosa Type VI Virulence Effector Tse1 Bacteriolysis and Self-protection Mechanisms. J Biol Chem. 287(32):26911-20. [PudMed:22700987] [14] Bleves S et al (2010) Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426] [15] Robb CS et al (2013) Structure of the T6SS lipoprotein TssJ1 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 6):607-10. [PudMed:23722835] [16] Forster A et al (2014) Coevolution of the ATPase ClpV, the Sheath Proteins TssB and TssC and the Accessory Protein TagJ/HsiE1 Distinguishes Type VI Secretion Classes. J Biol Chem. 289(47):33032-43. [PudMed:25305017] [17] Chou S et al (2012) Structure of a Peptidoglycan Amidase Effector Targeted to Gram-Negative Bacteria by the Type VI Secretion System. Cell Rep. 1(6):656-64. [PudMed:22813741] [18] Zhang H et al (2012) Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa. FEBS Lett. 586(19):3193-9. [PudMed:22750141] [19] Hood RD et al (2010) A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe. 7(1):25-37. [PudMed:20114026] [20] Basler M et al (2013) Tit-for-Tat: Type VI Secretion System Counterattack during Bacterial Cell-Cell Interactions. Cell. 152(4):884-94. [PudMed:23415234] [21] Russell AB et al (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature. 475(7356):343-7. [PudMed:21776080] [22] Whitney JC et al (2014) Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 92(3):529-42. [PudMed:24589350] [23] Casabona MG et al (2012) An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa. Environ Microbiol. 15(2):471-86. [PudMed:22765374] [24] Goldova J et al (2011) A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence. BMC Genomics. 0.803472222. [PudMed:21880152] [25] Lossi NS et al (2011) Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa. Microbiology. 157(Pt 12):3292-305. [PudMed:21873404] [26] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] [27] Hachani A et al (2011) Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275] [28] Mougous JD et al (2006) A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science. 312(5779):1526-30. [PudMed:16763151] |
T6SS components and genome coordinates
Component | FHA | PpkA | PppA | TagF | TagJ | TagQ | TagR | TagS | TagT | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 3 | 1 | 1 | 1 | 1 |
Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
---|---|---|---|---|---|---|---|
PA0067 (prlC) | 78710..80755 | + | 2046 | NP_248757.1 | oligopeptidase A | ||
PA0068 | 80752..81027 | + | 276 | NP_248758.1 | hypothetical protein | ||
PA0069 | 81116..82174 | + | 1059 | NP_248759.1 | hypothetical protein | ||
T6CP012414 | TagQ | PA0070 | 82404..83318 | - | 915 | NP_248760.1 | hypothetical protein |
T6CP012415 | TagR | PA0071 | 83380..85092 | - | 1713 | NP_248761.1 | hypothetical protein |
T6CP012416 | TagS | PA0072 | 85085..86284 | - | 1200 | NP_248762.1 | hypothetical protein |
T6CP012417 | TagT | PA0073 | 86284..87003 | - | 720 | NP_248763.1 | ABC transporter ATP-binding protein |
T6CP012418 | PpkA | PA0074 (ppkA) | 87000..90098 | - | 3099 | NP_248764.1 | serine/threonine protein kinase PpkA |
T6CP012419 | PppA | PA0075 (pppA) | 90106..90834 | - | 729 | NP_248765.1 | serine/threonine phosphatase |
T6CP012420 | TagF | PA0076 | 90844..91524 | - | 681 | NP_248766.1 | hypothetical protein |
T6CP005094 | TssM | PA0077 (icmF1) | 91521..94826 | - | 3306 | NP_248767.1 | type VI secretion protein IcmF |
T6CP005095 | TssL | PA0078 | 95048..96397 | - | 1350 | NP_248768.1 | hypothetical protein |
T6CP005096 | TssK | PA0079 | 96404..97738 | - | 1335 | NP_248769.1 | hypothetical protein |
T6CP005097 | TssJ | PA0080 | 97754..98218 | - | 465 | NP_248770.1 | hypothetical protein |
T6CP011390 | FHA | PA0081 (fha1) | 98263..99756 | - | 1494 | NP_248771.1 | Fha domain-containing protein |
T6CP005098 | TssA | PA0082 | 100124..101158 | + | 1035 | NP_248772.1 | hypothetical protein |
T6CP005099 | TssB | PA0083 | 101247..101765 | + | 519 | NP_248773.1 | hypothetical protein |
T6CP005100 | TssC | PA0084 | 101778..103274 | + | 1497 | NP_248774.1 | hypothetical protein |
T6CP005101 | TssD | PA0085 (hcp1) | 103350..103838 | + | 489 | NP_248775.1 | protein secretion apparatus assembly protein |
T6CP012694 | TagJ | PA0086 | 104006..104851 | + | 846 | NP_248776.1 | hypothetical protein |
T6CP005102 | TssE | PA0087 | 104853..105362 | + | 510 | NP_248777.1 | hypothetical protein |
T6CP005103 | TssF | PA0088 | 105359..107218 | + | 1860 | NP_248778.1 | hypothetical protein |
T6CP005104 | TssG | PA0089 | 107182..108228 | + | 1047 | NP_248779.1 | hypothetical protein |
T6CP005105 | TssH | PA0090 (clpV1) | 108221..110929 | + | 2709 | NP_248780.1 | secretion protein ClpV1 |
T6CP005106 | TssI | PA0091 (vgrG1) | 110976..112907 | + | 1932 | NP_248781.1 | type VI secretion system protein VgrG |
PA0092 | 113022..113306 | - | 285 | NP_248782.1 | hypothetical protein | ||
PA0093 | 113303..114595 | - | 1293 | NP_248783.1 | hypothetical protein | ||
PA0094 | 114611..115045 | - | 435 | NP_248784.1 | hypothetical protein | ||
T6CP005107 | TssI | PA0095 | 115299..117524 | + | 2226 | NP_248785.1 | hypothetical protein |
PA0096 | 117552..118001 | + | 450 | NP_248786.1 | hypothetical protein | ||
PA0097 | 117931..119130 | + | 1200 | NP_248787.1 | hypothetical protein | ||
PA0098 | 119127..120164 | + | 1038 | NP_248788.1 | 3-oxoacyl-ACP synthase | ||
PA0099 | 120164..121324 | + | 1161 | NP_248789.1 | hypothetical protein | ||
PA0100 | 121346..122266 | + | 921 | NP_248790.1 | hypothetical protein | ||
PA0101 | 122248..123495 | + | 1248 | NP_248791.1 | hypothetical protein | ||
PA0102 | 123871..124599 | + | 729 | NP_248792.1 | carbonic anhydrase | ||
PA0103 | 124810..126381 | + | 1572 | NP_248793.1 | sulfate transporter | ||
PA0103a | 126368..127006 | - | 639 | YP_008719730.1 | hypothetical protein |
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Experimental investigation has been performed.
Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
---|---|---|---|---|---|---|---|---|
ACP00002 | PA0094 (eagT6) | adaptor | Pseudomonas aeruginosa PAO1 | chromosome (NC_002516) | 114611..115045 (-) | 15595292 | - | PA0093 (Tse6) |
ACP00003 | PA0086 (tagJ) | structure affiliated protein | Pseudomonas aeruginosa PAO1 | chromosome (NC_002516) | 104006..104851 (+) | 15595284 | PA0082 (TssA) | - |
ACP00004 | PA0086 (tagJ) | structure affiliated protein | Pseudomonas aeruginosa PAO1 | chromosome (NC_002516) | 104006..104851 (+) | 15595284 | PA0083 (HisB1/TssB1) | - |