Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00003 experimental
T6SS type Type i3
Strain Pseudomonas aeruginosa PAO1
Replicon (RefSeq) chromosome (H1-T6SS) (NC_002516)
Location 83380..123495
Function anti-bacterial; anti-eukaryotic; effector translocation; virulence
Comment Virulence in rat model of chronic respiratory infection; Ability to outcompete a susceptible mutant of P. aeruginosa; ability to outcompete P. putida; growth inhibition of E. coli.
References
[1] Zou T et al (2012) Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin. J Mol Biol. 417(4):351-61. [PudMed:22310046]
[2] Silverman JM et al (2011) Separate inputs modulate phosphorylation-dependent and -independent type VI secretion activation. Mol Microbiol. 82(5):1277-90. [PudMed:22017253]
[3] Hachani A et al (2014) The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869]
[4] Osipiuk J et al (2011) Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
[5] Hsu F et al (2009) TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa. Mol Microbiol. 72(5):1111-25. [PudMed:19400797]
[6] Leroux M et al (2012) Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword. Proc Natl Acad Sci U S A. 109(48):19804-9. [PudMed:23150540]
[7] Li M et al (2012) Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathog. 8(4):e1002613. [PudMed:22511866]
[8] Mougous JD et al (2007) Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa. Nat Cell Biol. 9(7):797-803. [PudMed:17558395]
[9] Kefala K et al (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 6):695-7. [PudMed:22684073]
[10] Lossi NS et al (2012) The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system. Mol Microbiol. 86(2):437-56. [PudMed:22906320]
[11] Sana TG et al (2012) The Second Type VI Secretion System of Pseudomonas aeruginosa Strain PAO1 Is Regulated by Quorum Sensing and Fur and Modulates Internalization in Epithelial Cells. J Biol Chem. 287(32):27095-105. [PudMed:22665491]
[12] Lu D et al (2013) Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 5):524-7. [PudMed:23695568]
[13] Ding J et al (2012) Structural Insights into the Pseudomonas aeruginosa Type VI Virulence Effector Tse1 Bacteriolysis and Self-protection Mechanisms. J Biol Chem. 287(32):26911-20. [PudMed:22700987]
[14] Bleves S et al (2010) Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
[15] Robb CS et al (2013) Structure of the T6SS lipoprotein TssJ1 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 6):607-10. [PudMed:23722835]
[16] Forster A et al (2014) Coevolution of the ATPase ClpV, the Sheath Proteins TssB and TssC and the Accessory Protein TagJ/HsiE1 Distinguishes Type VI Secretion Classes. J Biol Chem. 289(47):33032-43. [PudMed:25305017]
[17] Chou S et al (2012) Structure of a Peptidoglycan Amidase Effector Targeted to Gram-Negative Bacteria by the Type VI Secretion System. Cell Rep. 1(6):656-64. [PudMed:22813741]
[18] Zhang H et al (2012) Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa. FEBS Lett. 586(19):3193-9. [PudMed:22750141]
[19] Hood RD et al (2010) A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe. 7(1):25-37. [PudMed:20114026]
[20] Basler M et al (2013) Tit-for-Tat: Type VI Secretion System Counterattack during Bacterial Cell-Cell Interactions. Cell. 152(4):884-94. [PudMed:23415234]
[21] Russell AB et al (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature. 475(7356):343-7. [PudMed:21776080]
[22] Whitney JC et al (2014) Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 92(3):529-42. [PudMed:24589350]
[23] Casabona MG et al (2012) An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa. Environ Microbiol. 15(2):471-86. [PudMed:22765374]
[24] Goldova J et al (2011) A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence. BMC Genomics. 0.803472222. [PudMed:21880152]
[25] Lossi NS et al (2011) Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa. Microbiology. 157(Pt 12):3292-305. [PudMed:21873404]
[26] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
[27] Hachani A et al (2011) Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275]
[28] Mougous JD et al (2006) A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science. 312(5779):1526-30. [PudMed:16763151]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHAPpkAPppATagFTagJTagQTagRTagSTagTTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number1111111111111111131111


Loading, please wait
Component ID
Component
Locus tag (Gene)
Coordinate
Strand
Size (bp)
NCBI ID
Product
PA0067 (prlC)78710..80755+2046NP_248757.1oligopeptidase A
PA006880752..81027+276NP_248758.1hypothetical protein
PA006981116..82174+1059NP_248759.1hypothetical protein
T6CP012414TagQPA007082404..83318-915NP_248760.1hypothetical protein
T6CP012415TagRPA007183380..85092-1713NP_248761.1hypothetical protein
T6CP012416TagSPA007285085..86284-1200NP_248762.1hypothetical protein
T6CP012417TagTPA007386284..87003-720NP_248763.1ABC transporter ATP-binding protein
T6CP012418PpkAPA0074 (ppkA)87000..90098-3099NP_248764.1serine/threonine protein kinase PpkA
T6CP012419PppAPA0075 (pppA)90106..90834-729NP_248765.1serine/threonine phosphatase
T6CP012420TagFPA007690844..91524-681NP_248766.1hypothetical protein
Showing 1 to 10 of 38 rows rows per page
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Loading, please wait
Regulator ID
Locus tag (Gene)
Regulator type
Regulatory pathway
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Target (Regulation)
REG0018 experimentalPA4462 (RpoN)proteinGlobal regulatorPseudomonas aeruginosa PAO1chromosome (NC_002516)4992870..4994363 (+)15599658PA1512 (hcpA) promotor (↓); PA5267 (hcpB) promotor (↓)
REG0040 experimentalPA0592 (RsmA)proteinRsmA regulatory pathwayPseudomonas aeruginosa PAO1chromosome (NC_002516)650575..651381 (-)15595789FHA (PA0081) (↑)
REG0041 experimentalPA5183a (RsmN)proteinRsmA regulatory pathwayPseudomonas aeruginosa PAO1chromosome (NC_002516)5836470..5836685 (-)554753081FHA (PA0081) (↑); PA3487 (pldA) expression (↑)
REG0042 experimentalPA0075 (PppA)proteinTppPseudomonas aeruginosa PAO1chromosome (NC_002516)90106..90834 (-)15595273PA0081 (Fha-1/TagH) (↑)
REG0043 experimentalPA0074 (PpkA)proteinTppPseudomonas aeruginosa PAO1chromosome (NC_002516)87000..90098 (-)15595272PA0081 (Fha-1/TagH) (↓)
REG0044 experimentalPA2586 (gacA)proteinTCS-RRPseudomonas aeruginosa PAO1chromosome (NC_002516)2925788..2926432 (-)15597782hcp1 (PA0085) expression and secretion (↓)
REG0045 experimentalPA0610 (prtN)proteinTCS-HKPseudomonas aeruginosa PAO1chromosome (NC_002516)672777..673091 (-)15595807PA0611 (prtR) (↑)
REG0046 experimentalPA0611 (prtR)proteinTCS-RRPseudomonas aeruginosa PAO1chromosome (NC_002516)673191..673961 (-)15595808hcp1 (PA0085) expression and secretion (↑); PA2586 (gacA) (↑)
REG0047 experimentalPA0072 (TagS)proteinTppPseudomonas aeruginosa PAO1chromosome (NC_002516)85085..86284 (-)15595270PA0073 (TagT) (↓)
REG0048 experimentalPA0070 (TagQ)proteinTppPseudomonas aeruginosa PAO1chromosome (NC_002516)82404..83318 (-)15595268PA0071 (tagR) (↓)
Showing 1 to 10 of 25 rows rows per page

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.

experimental Experimental investigation has been performed.
Loading, please wait
Accessory protein ID
Locus tag (Gene)
Type            
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Related structure protein
Related effector
ACP00002 experimentalPA0094 (eagT6)adaptorPseudomonas aeruginosa PAO1chromosome (NC_002516)114611..115045 (-)15595292-PA0093 (Tse6)
ACP00003 experimentalPA0086 (tagJ)structure affiliated proteinPseudomonas aeruginosa PAO1chromosome (NC_002516)104006..104851 (+)15595284PA0082 (TssA)-
ACP00004 experimentalPA0086 (tagJ)structure affiliated proteinPseudomonas aeruginosa PAO1chromosome (NC_002516)104006..104851 (+)15595284PA0083 (HisB1/TssB1)-
Showing 1 to 3 of 3 rows