Detailed information of effector
Summary
| Effector ID | EFF00017  |
| Effector type | Tge |
| Cognate immunity protein | IMU00191 |
| Related T6SS (Type) | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (409 a.a.); Nucleotide sequence (1227 bp) |
| Description | Tse3 (Tge1); muramidase |
| Reference | [1] Hood RD, Singh P, Hsu F, et al. A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe. 2010 Jan 21;7(1):25-37. doi: 10.1016/j.chom.2009.12.007. PMID: 20114026 [2] Russell AB, Hood RD, Bui NK, et al. Type VI secretion delivers bacteriolytic effectors to target cells. Nature. 2011 Jul 20;475(7356):343-7. doi: 10.1038/nature10244. PMID: 21776080 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA3484 (Tse3, Tge1) |
| Coordinates (Strand) | 3898278..3899504 (+) |
| NCBI ID | 15598680 |
| RefSeq | NC_002516 |
| Uniprot ID | TSE3_PSEAE |
| KEGG ID | pae:PA3484 |
| PDB ID | 4N88, 3WA5, 4LUQ, 4N7S, 4N80, 4M5E, 4M5F |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-408 | MTATSDLIESLISYSWDDWQVTRQEARRVIAAIRNDNVPDATIAALDKSGSLIKLFQRVGPPELARSLIASIAGRTTMQRYQARNALIRSLINNPLGTQTDNWIYFPTITFFDICADLADAAGRLGFAAAGATGVASQAIQGPFSGVGATGVNPTDLPSIAFGDQLKLLNKDPATVTKYSNPLGDLGAYLSQLSPQDKLNQAQTLVGQPISTLFPDAYPGNPPSRAKVMSAAARKYDLTPQLIGAIILAEQRDQTRDEDAKDYQAAVSIKSANTSIGLGQVVVSTAIKYELFTDLLGQPVRRGLSRKAVATLLASDEFNIFATARYIRYVANLASQQDLRKLPKTRGAFPSIDLRAYAGNPRNWPRDNVRALASEYTSRPWDDNLSPGWPMFVDDAYATFLDPGMRFP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9937 | - | - |
Protein sequence: 409 a.a. .
>EFF00017 NC_002516:3898278-3899504 Type VI Glycoside hydrolase Effector (Tge) [Pseudomonas aeruginosa PAO1]
MTATSDLIESLISYSWDDWQVTRQEARRVIAAIRNDNVPDATIAALDKSGSLIKLFQRVGPPELARSLIASIAGRTTMQR
YQARNALIRSLINNPLGTQTDNWIYFPTITFFDICADLADAAGRLGFAAAGATGVASQAIQGPFSGVGATGVNPTDLPSI
AFGDQLKLLNKDPATVTKYSNPLGDLGAYLSQLSPQDKLNQAQTLVGQPISTLFPDAYPGNPPSRAKVMSAAARKYDLTP
QLIGAIILAEQRDQTRDEDAKDYQAAVSIKSANTSIGLGQVVVSTAIKYELFTDLLGQPVRRGLSRKAVATLLASDEFNI
FATARYIRYVANLASQQDLRKLPKTRGAFPSIDLRAYAGNPRNWPRDNVRALASEYTSRPWDDNLSPGWPMFVDDAYATF
LDPGMRFP*
MTATSDLIESLISYSWDDWQVTRQEARRVIAAIRNDNVPDATIAALDKSGSLIKLFQRVGPPELARSLIASIAGRTTMQR
YQARNALIRSLINNPLGTQTDNWIYFPTITFFDICADLADAAGRLGFAAAGATGVASQAIQGPFSGVGATGVNPTDLPSI
AFGDQLKLLNKDPATVTKYSNPLGDLGAYLSQLSPQDKLNQAQTLVGQPISTLFPDAYPGNPPSRAKVMSAAARKYDLTP
QLIGAIILAEQRDQTRDEDAKDYQAAVSIKSANTSIGLGQVVVSTAIKYELFTDLLGQPVRRGLSRKAVATLLASDEFNI
FATARYIRYVANLASQQDLRKLPKTRGAFPSIDLRAYAGNPRNWPRDNVRALASEYTSRPWDDNLSPGWPMFVDDAYATF
LDPGMRFP*
Nucleotide sequence: 1227 bp .
>EFF00017 NC_002516:3898278-3899504 Type VI Glycoside hydrolase Effector (Tge) [Pseudomonas aeruginosa PAO1]
ATGACCGCCACCAGCGACCTGATCGAGTCACTGATTTCCTATAGCTGGGACGACTGGCAGGTGACCCGCCAGGAAGCCCG
CCGGGTGATCGCCGCGATCCGCAACGACAACGTGCCCGATGCGACCATCGCCGCACTCGACAAGAGCGGCTCGCTGATCA
AGCTGTTCCAGCGGGTGGGCCCGCCGGAACTGGCGCGCTCGCTGATCGCCAGCATCGCCGGGCGCACCACCATGCAGCGC
TACCAGGCACGCAATGCCTTGATCCGCAGCCTGATCAATAATCCCCTGGGCACCCAGACCGACAACTGGATCTACTTCCC
CACCATCACCTTCTTCGACATCTGCGCCGACCTCGCCGACGCCGCTGGCCGCCTGGGCTTCGCCGCGGCCGGCGCCACCG
GGGTGGCCAGCCAGGCGATCCAGGGTCCGTTCAGCGGGGTCGGCGCCACCGGCGTCAATCCGACCGACCTGCCGTCCATT
GCCTTCGGCGACCAGCTCAAGCTGCTCAACAAGGACCCGGCGACCGTCACCAAGTACAGCAACCCGCTCGGCGACCTGGG
CGCCTACCTGAGCCAGCTTTCGCCGCAAGACAAGCTGAACCAGGCACAGACGCTGGTCGGCCAGCCGATCAGCACGCTGT
TCCCCGACGCCTATCCGGGCAACCCGCCGTCGCGGGCCAAGGTCATGTCCGCGGCCGCGCGCAAGTACGACCTGACGCCG
CAACTGATCGGCGCGATCATCCTCGCCGAGCAGCGTGACCAGACCCGCGACGAAGACGCCAAGGACTATCAGGCGGCAGT
CAGCATCAAGAGCGCCAACACCTCCATCGGCCTCGGCCAGGTGGTGGTCTCCACCGCGATCAAGTACGAGCTGTTCACCG
ACCTGCTCGGCCAGCCGGTGCGCCGCGGTCTGTCGCGCAAGGCGGTCGCCACCCTGCTGGCTTCCGACGAATTCAACATC
TTCGCCACCGCCCGTTACATCCGCTACGTCGCCAACCTCGCGTCGCAACAGGACCTGCGCAAGTTGCCGAAGACCCGCGG
CGCATTTCCCTCCATCGATCTCCGCGCCTACGCCGGCAATCCGCGCAACTGGCCGCGGGACAATGTCCGCGCGCTGGCCT
CGGAATACACCTCGCGGCCCTGGGACGACAACCTGTCGCCGGGCTGGCCGATGTTCGTCGACGATGCCTACGCCACCTTC
CTCGACCCTGGAATGAGGTTCCCATGA
ATGACCGCCACCAGCGACCTGATCGAGTCACTGATTTCCTATAGCTGGGACGACTGGCAGGTGACCCGCCAGGAAGCCCG
CCGGGTGATCGCCGCGATCCGCAACGACAACGTGCCCGATGCGACCATCGCCGCACTCGACAAGAGCGGCTCGCTGATCA
AGCTGTTCCAGCGGGTGGGCCCGCCGGAACTGGCGCGCTCGCTGATCGCCAGCATCGCCGGGCGCACCACCATGCAGCGC
TACCAGGCACGCAATGCCTTGATCCGCAGCCTGATCAATAATCCCCTGGGCACCCAGACCGACAACTGGATCTACTTCCC
CACCATCACCTTCTTCGACATCTGCGCCGACCTCGCCGACGCCGCTGGCCGCCTGGGCTTCGCCGCGGCCGGCGCCACCG
GGGTGGCCAGCCAGGCGATCCAGGGTCCGTTCAGCGGGGTCGGCGCCACCGGCGTCAATCCGACCGACCTGCCGTCCATT
GCCTTCGGCGACCAGCTCAAGCTGCTCAACAAGGACCCGGCGACCGTCACCAAGTACAGCAACCCGCTCGGCGACCTGGG
CGCCTACCTGAGCCAGCTTTCGCCGCAAGACAAGCTGAACCAGGCACAGACGCTGGTCGGCCAGCCGATCAGCACGCTGT
TCCCCGACGCCTATCCGGGCAACCCGCCGTCGCGGGCCAAGGTCATGTCCGCGGCCGCGCGCAAGTACGACCTGACGCCG
CAACTGATCGGCGCGATCATCCTCGCCGAGCAGCGTGACCAGACCCGCGACGAAGACGCCAAGGACTATCAGGCGGCAGT
CAGCATCAAGAGCGCCAACACCTCCATCGGCCTCGGCCAGGTGGTGGTCTCCACCGCGATCAAGTACGAGCTGTTCACCG
ACCTGCTCGGCCAGCCGGTGCGCCGCGGTCTGTCGCGCAAGGCGGTCGCCACCCTGCTGGCTTCCGACGAATTCAACATC
TTCGCCACCGCCCGTTACATCCGCTACGTCGCCAACCTCGCGTCGCAACAGGACCTGCGCAAGTTGCCGAAGACCCGCGG
CGCATTTCCCTCCATCGATCTCCGCGCCTACGCCGGCAATCCGCGCAACTGGCCGCGGGACAATGTCCGCGCGCTGGCCT
CGGAATACACCTCGCGGCCCTGGGACGACAACCTGTCGCCGGGCTGGCCGATGTTCGTCGACGATGCCTACGCCACCTTC
CTCGACCCTGGAATGAGGTTCCCATGA