Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00191  |
| Cognate effector | EFF00017 |
| Related T6SS (Type) | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (146 a.a.); Nucleotide sequence (438 bp) |
| Description | Tsi3 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA3485 (Tsi3,Tgi1) |
| Coordinates (Strand) | 3899501..3899938 (+) |
| NCBI ID | 15598681 |
| RefSeq | NC_002516 |
| Uniprot ID | TSI3_PSEAE |
| KEGG ID | pae:PA3485 |
| PDB ID | 4N88, 3WA5, 4LUQ, 4N7S, 4N80, 4M5F |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-145 | MKTVALILASLALLACTAESGVDFDKTLTHPNGLVVERPVGFDARRSAEGFRFDEGGKLRNPRQLEVQRQDAPPPPDLASRRLGDGEARYKVEEDDGGSAGSEYRLWAAKPAGARWIVVSASEQSEDGEPTFALAWALLERARLQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9873 | pos: 15-16. LLA-CT. | MKTVALILASLALLA |
Protein sequence: 146 a.a. .
>IMU00191 NC_002516:3899501-3899938 [Pseudomonas aeruginosa PAO1]
MKTVALILASLALLACTAESGVDFDKTLTHPNGLVVERPVGFDARRSAEGFRFDEGGKLRNPRQLEVQRQDAPPPPDLAS
RRLGDGEARYKVEEDDGGSAGSEYRLWAAKPAGARWIVVSASEQSEDGEPTFALAWALLERARLQ*
MKTVALILASLALLACTAESGVDFDKTLTHPNGLVVERPVGFDARRSAEGFRFDEGGKLRNPRQLEVQRQDAPPPPDLAS
RRLGDGEARYKVEEDDGGSAGSEYRLWAAKPAGARWIVVSASEQSEDGEPTFALAWALLERARLQ*
Nucleotide sequence: 438 bp .
>IMU00191 NC_002516:3899501-3899938 [Pseudomonas aeruginosa PAO1]
ATGAAGACAGTCGCCCTGATTCTCGCCAGCCTCGCCCTGCTGGCCTGCACCGCGGAGTCCGGGGTGGACTTCGACAAGAC
CCTGACCCACCCGAACGGCCTGGTGGTGGAGCGCCCCGTCGGCTTCGACGCGCGGCGCAGCGCCGAGGGCTTCCGCTTCG
ACGAAGGCGGCAAGCTGCGCAACCCACGCCAACTGGAGGTCCAGCGCCAGGACGCGCCGCCCCCGCCCGACCTGGCGAGC
AGGCGCCTGGGCGACGGCGAGGCGCGCTACAAGGTAGAGGAAGACGACGGCGGCTCCGCCGGCAGCGAATACCGCCTGTG
GGCAGCCAAGCCGGCGGGCGCGCGGTGGATCGTGGTCAGCGCCAGCGAGCAGAGCGAGGATGGCGAACCGACCTTCGCCC
TGGCCTGGGCGCTGCTGGAGCGGGCCAGGTTGCAATAG
ATGAAGACAGTCGCCCTGATTCTCGCCAGCCTCGCCCTGCTGGCCTGCACCGCGGAGTCCGGGGTGGACTTCGACAAGAC
CCTGACCCACCCGAACGGCCTGGTGGTGGAGCGCCCCGTCGGCTTCGACGCGCGGCGCAGCGCCGAGGGCTTCCGCTTCG
ACGAAGGCGGCAAGCTGCGCAACCCACGCCAACTGGAGGTCCAGCGCCAGGACGCGCCGCCCCCGCCCGACCTGGCGAGC
AGGCGCCTGGGCGACGGCGAGGCGCGCTACAAGGTAGAGGAAGACGACGGCGGCTCCGCCGGCAGCGAATACCGCCTGTG
GGCAGCCAAGCCGGCGGGCGCGCGGTGGATCGTGGTCAGCGCCAGCGAGCAGAGCGAGGATGGCGAACCGACCTTCGCCC
TGGCCTGGGCGCTGCTGGAGCGGGCCAGGTTGCAATAG