Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00002  |
| Type | adaptor |
| Related T6SS (Type) | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Related structure protein | - |
| Related effector | PA0093 (Tse6) |
| Description | EagT6, a Tse6 specific chaperone |
| Reference | [1] Whitney JC, Quentin D, Sawai S, et al. An interbacterial NAD(P)(+) glycohydrolase toxin requires elongation factor Tu for delivery to target cells. Cell. 2015 Oct 22;163(3):607-19. doi: 10.1016/j.cell.2015.09.027. Epub 2015 Oct 8. PMID: 26456113 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA0094 (eagT6) |
| Coordinates (Strand) | 114611..115045 (-) |
| NCBI ID | 15595292 |
| RefSeq | NC_002516 |
| Uniprot ID | EAGT6_PSEAE |
| KEGG ID | pae:PA0094 |
| PDB ID | 1TU1, 6XRF |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DcrB | PF08786.13 | 2.1e-35 | 8..136 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-144 | VTLYRLHEADLEIPDAWQDQSINIFKLPASGPAREASFVISRDASQGDAPFADYVARQLENAEKQLPGFKLHKRWDINIHGHAAVLLDYQWQREGRDLMLRQVFIERRPAVLITTLTTTPADLPHHEPAWKQAMQTLVPRPTPS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9903 | - | - |
Protein sequence: 145 a.a.
>ACP00002 NC_002516:c115045-114611 [Pseudomonas aeruginosa PAO1]
VTLYRLHEADLEIPDAWQDQSINIFKLPASGPAREASFVISRDASQGDAPFADYVARQLENAEKQLPGFKLHKRWDINIH
GHAAVLLDYQWQREGRDLMLRQVFIERRPAVLITTLTTTPADLPHHEPAWKQAMQTLVPRPTPS*
VTLYRLHEADLEIPDAWQDQSINIFKLPASGPAREASFVISRDASQGDAPFADYVARQLENAEKQLPGFKLHKRWDINIH
GHAAVLLDYQWQREGRDLMLRQVFIERRPAVLITTLTTTPADLPHHEPAWKQAMQTLVPRPTPS*
Nucleotide sequence: 435 bp
>ACP00002 NC_002516:c115045-114611 [Pseudomonas aeruginosa PAO1]
GTGACGCTCTATCGCCTCCACGAAGCCGATCTCGAGATTCCCGATGCCTGGCAGGACCAGAGCATCAACATCTTCAAGCT
CCCCGCCAGCGGCCCCGCCCGCGAAGCCAGTTTCGTCATCAGCCGTGACGCCAGCCAGGGCGACGCGCCGTTCGCCGACT
ATGTCGCCCGCCAACTGGAAAACGCCGAGAAGCAATTGCCCGGCTTCAAGCTGCACAAGCGCTGGGACATCAACATCCAC
GGCCACGCCGCCGTGCTCCTCGACTACCAGTGGCAGCGCGAGGGCCGCGACCTGATGCTGCGCCAGGTATTCATCGAGCG
CCGCCCGGCGGTGCTGATCACCACCCTGACCACCACCCCGGCTGACCTGCCGCACCACGAGCCGGCCTGGAAGCAGGCGA
TGCAGACCCTGGTCCCGCGCCCGACCCCTAGCTGA
GTGACGCTCTATCGCCTCCACGAAGCCGATCTCGAGATTCCCGATGCCTGGCAGGACCAGAGCATCAACATCTTCAAGCT
CCCCGCCAGCGGCCCCGCCCGCGAAGCCAGTTTCGTCATCAGCCGTGACGCCAGCCAGGGCGACGCGCCGTTCGCCGACT
ATGTCGCCCGCCAACTGGAAAACGCCGAGAAGCAATTGCCCGGCTTCAAGCTGCACAAGCGCTGGGACATCAACATCCAC
GGCCACGCCGCCGTGCTCCTCGACTACCAGTGGCAGCGCGAGGGCCGCGACCTGATGCTGCGCCAGGTATTCATCGAGCG
CCGCCCGGCGGTGCTGATCACCACCCTGACCACCACCCCGGCTGACCTGCCGCACCACGAGCCGGCCTGGAAGCAGGCGA
TGCAGACCCTGGTCCCGCGCCCGACCCCTAGCTGA