Detailed information of regulator
Summary
| Regulator ID | REG0045  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS-HK |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (105 a.a.); Nucleotide sequence (315 bp) |
| Description | The promoter activities of GacS and GacA were increased in the ΔprtNΔprtR mutant and western blot assay showed that the protein levels of GacS and GacA were more abundance in the ΔprtNΔprtR mutant compared to ΔprtN strain. Hcp1 were examined in the wild-type PAO1, ΔprtN, ΔprtNΔprtR,ΔprtNΔprtRΔgacA as well as the complemented strains. The deletion of gacA in the ΔprtNΔprtR strain restored the expression levels of ExoS to those in the ΔprtN mutant and wild-type parent but more fewer for Hcp1. |
| Reference |
External database links
| Locus tag (Gene) | PA0610 (prtN) |
| Coordinates (Strand) | 672777..673091 (-) |
| NCBI ID | 15595807 |
| RefSeq | NC_002516 |
| Uniprot ID | PRTN_PSEAE |
| KEGG ID | pae:PA0610 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| PA0611 (prtR) | ↑ | 33168590 |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| PyocinActivator | PF11112.10 | 1.9e-30 | 16..89 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-104 | MQPSIAPSTPIPRQETVELVYRIFGDVLVPLEQVRERWFRNLNKENFSKALACGRIALPVTTLDDSHKAMQFVALDHLAAYVDQRANQAGSARRAQPGADSIAS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9898 | - | - |
Protein sequence of regulator: 105 a.a. .
>REG0045 NC_002516:c673091-672777 [Pseudomonas aeruginosa PAO1]
MQPSIAPSTPIPRQETVELVYRIFGDVLVPLEQVRERWFRNLNKENFSKALACGRIALPVTTLDDSHKAMQFVALDHLAA
YVDQRANQAGSARRAQPGADSIAS*
MQPSIAPSTPIPRQETVELVYRIFGDVLVPLEQVRERWFRNLNKENFSKALACGRIALPVTTLDDSHKAMQFVALDHLAA
YVDQRANQAGSARRAQPGADSIAS*
Nucleotide sequence of regulator: 315 bp .
>REG0045 NC_002516:c673091-672777 [Pseudomonas aeruginosa PAO1]
ATGCAGCCATCCATCGCCCCTAGCACTCCGATTCCACGCCAGGAAACCGTGGAATTGGTCTACCGCATCTTCGGCGACGT
CCTGGTGCCGCTGGAGCAGGTTCGCGAACGCTGGTTTCGCAACCTGAACAAGGAAAACTTCAGCAAGGCCCTGGCATGCG
GGCGGATCGCCCTGCCGGTGACAACCCTCGACGATAGTCACAAGGCCATGCAGTTCGTGGCCCTCGATCACCTCGCCGCC
TACGTCGACCAGCGCGCGAACCAAGCCGGAAGCGCCCGTCGAGCACAGCCGGGCGCTGACAGCATCGCATCCTGA
ATGCAGCCATCCATCGCCCCTAGCACTCCGATTCCACGCCAGGAAACCGTGGAATTGGTCTACCGCATCTTCGGCGACGT
CCTGGTGCCGCTGGAGCAGGTTCGCGAACGCTGGTTTCGCAACCTGAACAAGGAAAACTTCAGCAAGGCCCTGGCATGCG
GGCGGATCGCCCTGCCGGTGACAACCCTCGACGATAGTCACAAGGCCATGCAGTTCGTGGCCCTCGATCACCTCGCCGCC
TACGTCGACCAGCGCGCGAACCAAGCCGGAAGCGCCCGTCGAGCACAGCCGGGCGCTGACAGCATCGCATCCTGA