Detailed information of regulator


Summary

Regulator ID REG0048 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (305 a.a.); Nucleotide sequence (915 bp)
Description Individual isogenic PAO1 mutants (ΔtagT, ΔtagS and ΔtagQ) were impaired in Hcp1 export and TagQ is an outer membrane lipoprotein that localizes independent of TagTS and is essential for OM localization of TagR. TagT, TagS and TagQ act upstream of PpkA in the TPP.
Reference
External database links

Locus tag (Gene) PA0070 (TagQ)
Coordinates (Strand) 82404..83318 (-)
NCBI ID 15595268
RefSeq NC_002516
Uniprot ID Q9I762_PSEAE
KEGG ID pae:PA0070
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
PA0071 (tagR) ↓ 15595269
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Gly-zipper_Omp PF13488.8 5.9e-06 70..116
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-304 MSQPSENRLITSARYALCLLTASGVLLSGCASSGVGSVAQTTRAEYYPSCYEPVSHLRSTDNAVRNSAITGAITGGLLGGLAGGLASDENRGRNAALAAAGGALAGGAAGYYMEKQKQISDDRARIGSYGTDVDRSTVEINRSVAYAKSAQSCYQSQFKALLDGRKNKSINEAEGRKRLAEIVSGLQETNALLVAANGRAGENISNYTQAYEKDLQQVGVPRAEVTKVAEAENRASTTKGGSKPKTGSNPKVPKEAVATEQTIRKAQDAQSEGNKVASQGQGMIREVCNSPDMGDWAPPSCAKA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9869 pos: 29-30. LSG-CA. MSQPSENRLITSARYALCLLTASGVLLSG


  Protein sequence of regulator: 305 a.a.    .

>REG0048 NC_002516:c83318-82404 [Pseudomonas aeruginosa PAO1]
MSQPSENRLITSARYALCLLTASGVLLSGCASSGVGSVAQTTRAEYYPSCYEPVSHLRSTDNAVRNSAITGAITGGLLGG
LAGGLASDENRGRNAALAAAGGALAGGAAGYYMEKQKQISDDRARIGSYGTDVDRSTVEINRSVAYAKSAQSCYQSQFKA
LLDGRKNKSINEAEGRKRLAEIVSGLQETNALLVAANGRAGENISNYTQAYEKDLQQVGVPRAEVTKVAEAENRASTTKG
GSKPKTGSNPKVPKEAVATEQTIRKAQDAQSEGNKVASQGQGMIREVCNSPDMGDWAPPSCAKA*

  Nucleotide sequence of regulator: 915 bp    .

>REG0048 NC_002516:c83318-82404 [Pseudomonas aeruginosa PAO1]
ATGAGCCAACCCAGCGAAAACCGTTTGATCACCTCGGCGCGCTACGCGCTCTGCCTGTTGACCGCCAGCGGCGTGCTGCT
CAGCGGCTGCGCCAGCAGCGGCGTCGGCTCGGTCGCCCAGACCACCCGCGCGGAATACTACCCGTCCTGCTACGAGCCGG
TGTCGCACCTGCGCAGCACCGATAACGCAGTGCGCAATTCGGCCATCACCGGCGCCATTACCGGCGGCCTCCTGGGCGGC
CTGGCCGGCGGCCTGGCCAGCGACGAGAACCGCGGCCGCAATGCCGCCCTCGCTGCCGCAGGCGGCGCCCTGGCCGGCGG
CGCGGCGGGCTACTACATGGAGAAGCAGAAGCAGATCAGCGATGACCGCGCGCGCATCGGCTCCTACGGTACCGACGTCG
ACCGCAGCACCGTCGAGATCAACCGTAGCGTGGCCTACGCCAAGTCGGCGCAAAGCTGCTACCAGAGCCAGTTCAAGGCT
CTGCTCGACGGTCGCAAGAACAAGTCGATCAACGAAGCCGAAGGGCGCAAGCGCCTGGCCGAGATCGTCAGCGGCCTGCA
GGAGACCAACGCCTTGCTGGTCGCCGCCAACGGCCGTGCCGGCGAGAACATCAGCAACTACACCCAGGCCTACGAGAAAG
ACCTGCAGCAGGTCGGCGTACCGCGCGCCGAGGTGACCAAGGTCGCCGAGGCCGAGAACCGCGCCAGCACTACGAAAGGC
GGCAGCAAGCCCAAGACCGGCAGCAATCCCAAGGTGCCGAAGGAAGCGGTCGCCACCGAGCAGACCATCCGCAAGGCCCA
GGACGCGCAAAGCGAAGGCAACAAGGTGGCCTCCCAGGGCCAGGGCATGATCCGGGAAGTCTGCAACAGCCCGGACATGG
GCGACTGGGCGCCGCCGAGCTGCGCCAAGGCCTGA