Detailed information of regulator


Summary

Regulator ID REG0046 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-RR
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (257 a.a.); Nucleotide sequence (771 bp)
Description The promoter activities of GacS and GacA were increased in the ΔprtNΔprtR mutant and western blot assay showed that the protein levels of GacS and GacA were more abundance in the ΔprtNΔprtR mutant compared to ΔprtN strain. Hcp1 were examined in the wild-type PAO1, ΔprtN, ΔprtNΔprtR,ΔprtNΔprtRΔgacA as well as the complemented strains. The deletion of gacA in the ΔprtNΔprtR strain restored the expression levels of ExoS to those in the ΔprtN mutant and wild-type parent but more fewer for Hcp1.
Reference
External database links

Locus tag (Gene) PA0611 (prtR)
Coordinates (Strand) 673191..673961 (-)
NCBI ID 15595808
RefSeq NC_002516
Uniprot ID PRTR_PSEAE
KEGG ID pae:PA0611
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
hcp1 (PA0085) expression and secretion↑-
PA2586 (gacA)↑33168590
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
Peptidase_S24PF00717.253.7e-24112..241
HTH_3PF01381.243.6e-1116..69
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-256MDKSTQIPPDSFAARLKQAMAMRNLKQETLAEAAGVSQNTIHKLTSGKAQSTRKLIEIAAALGVSPVWLQTGEGAPAARSAVSVADGSPLVLEPLHPWDSDTPLDEDEVELPLYKEVEMSAGAGRTAVREIEGRKLRFSYATLRASGVDPSAAICAQLTGNSMEPLIMDGSTIGVDTATTHITDGEIYALEHDGMLRVKFVYRLPGGGIRLRSFNREEYPDEEYSPEDMRSRQISMIGWVFWWSTVRHRRGPSLVR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9562--


  Protein sequence of regulator: 257 a.a.    .

>REG0046 NC_002516:c673961-673191 [Pseudomonas aeruginosa PAO1]
MDKSTQIPPDSFAARLKQAMAMRNLKQETLAEAAGVSQNTIHKLTSGKAQSTRKLIEIAAALGVSPVWLQTGEGAPAARS
AVSVADGSPLVLEPLHPWDSDTPLDEDEVELPLYKEVEMSAGAGRTAVREIEGRKLRFSYATLRASGVDPSAAICAQLTG
NSMEPLIMDGSTIGVDTATTHITDGEIYALEHDGMLRVKFVYRLPGGGIRLRSFNREEYPDEEYSPEDMRSRQISMIGWV
FWWSTVRHRRGPSLVR*

  Nucleotide sequence of regulator: 771 bp    .

>REG0046 NC_002516:c673961-673191 [Pseudomonas aeruginosa PAO1]
ATGGACAAGAGCACCCAGATCCCGCCCGACAGCTTCGCCGCTCGCCTCAAGCAGGCCATGGCGATGCGCAACCTGAAGCA
GGAAACCCTCGCCGAAGCGGCAGGGGTTTCGCAGAACACCATTCACAAGCTGACCTCGGGCAAGGCCCAGAGCACCCGCA
AGCTGATCGAGATCGCGGCGGCCCTGGGCGTCTCGCCGGTCTGGCTGCAGACCGGCGAAGGCGCTCCAGCCGCGCGCAGT
GCCGTGTCCGTGGCCGATGGCAGCCCATTGGTGCTGGAACCGCTGCATCCGTGGGACAGCGACACACCGCTGGACGAAGA
CGAAGTGGAACTGCCGCTGTACAAGGAAGTGGAGATGTCCGCCGGCGCCGGACGCACTGCGGTGCGCGAGATAGAGGGGC
GCAAGCTGCGTTTTTCCTACGCCACGCTGCGTGCCTCGGGCGTCGATCCGTCGGCGGCGATCTGCGCCCAACTCACCGGC
AACAGCATGGAACCGCTGATCATGGATGGCTCCACCATCGGCGTGGACACCGCCACCACCCATATCACCGATGGCGAGAT
CTACGCCCTCGAACATGACGGCATGCTGCGGGTGAAGTTCGTCTATCGCCTGCCCGGCGGCGGCATTCGCCTGCGCAGCT
TCAACCGCGAGGAATATCCGGACGAGGAGTACTCGCCGGAGGACATGCGCAGCCGCCAGATCAGCATGATCGGTTGGGTC
TTCTGGTGGTCCACCGTACGCCACCGGCGCGGCCCGTCCCTGGTGCGGTGA