Detailed information of regulator


Summary

Regulator ID REG0044 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-RR
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Biofilm formation - Escherichia coli (map02026)
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (215 a.a.); Nucleotide sequence (645 bp)
Description The promoter activities of GacS and GacA were increased in the ΔprtNΔprtR mutant and western blot assay showed that the protein levels of GacS and GacA were more abundance in the ΔprtNΔprtR mutant compared to ΔprtN strain. Hcp1 were examined in the wild-type PAO1, ΔprtN, ΔprtNΔprtR,ΔprtNΔprtRΔgacA as well as the complemented strains. The deletion of gacA in the ΔprtNΔprtR strain restored the expression levels of ExoS to those in the ΔprtN mutant and wild-type parent but more fewer for Hcp1.
Reference
External database links

Locus tag (Gene) PA2586 (gacA)
Coordinates (Strand) 2925788..2926432 (-)
NCBI ID 15597782
RefSeq NC_002516
Uniprot ID GACA_PSEAE
KEGG ID pae:PA2586
KEGG pathway (determined by BlastKOALA) map02020; map05111; map02025; map02026
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
hcp1 (PA0085) expression and secretion ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Response_reg PF00072.26 2.8e-28 4..116
GerE PF00196.21 1.2e-14 148..204
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-214 VIKVLVVDDHDLVRTGITRMLADIEGLQVVGQADCGEDCLKLARELKPDVVLMDVKMPGIGGLEATRKLLRSQPDIKVVVVTVCEEDPFPTRLMQAGAAGYMTKGAGLEEMVQAIRQVFAGQRYISPQIAQQLALKSFQPQQHDSPFDSLSEREIQIALMIANCHKVQSISDKLCLSPKTVNTYRYRIFEKLSITSDVELALLAVRHGMVDAAS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9941 - -


  Protein sequence of regulator: 215 a.a.    .

>REG0044 NC_002516:c2926432-2925788 [Pseudomonas aeruginosa PAO1]
VIKVLVVDDHDLVRTGITRMLADIEGLQVVGQADCGEDCLKLARELKPDVVLMDVKMPGIGGLEATRKLLRSQPDIKVVV
VTVCEEDPFPTRLMQAGAAGYMTKGAGLEEMVQAIRQVFAGQRYISPQIAQQLALKSFQPQQHDSPFDSLSEREIQIALM
IANCHKVQSISDKLCLSPKTVNTYRYRIFEKLSITSDVELALLAVRHGMVDAAS*

  Nucleotide sequence of regulator: 645 bp    .

>REG0044 NC_002516:c2926432-2925788 [Pseudomonas aeruginosa PAO1]
GTGATTAAGGTGCTGGTGGTCGACGACCACGATCTGGTACGCACCGGTATTACCCGCATGCTGGCCGACATCGAAGGCTT
GCAAGTGGTCGGCCAGGCCGACTGCGGTGAAGACTGTCTGAAACTGGCCCGCGAACTGAAGCCGGATGTCGTCCTGATGG
ACGTGAAGATGCCCGGTATCGGCGGCCTGGAGGCGACCCGCAAGCTGCTGCGCAGCCAGCCCGACATCAAGGTCGTGGTA
GTCACCGTCTGCGAAGAGGATCCGTTCCCCACCCGCCTCATGCAGGCCGGCGCCGCCGGCTACATGACCAAGGGCGCGGG
GCTGGAGGAAATGGTCCAGGCGATTCGCCAGGTCTTCGCCGGCCAGCGCTATATCAGCCCGCAGATCGCCCAGCAACTGG
CGCTGAAGTCCTTCCAGCCGCAGCAGCACGATTCCCCCTTCGATTCGCTGTCCGAGCGCGAGATCCAGATCGCCCTGATG
ATCGCCAACTGCCACAAGGTGCAGAGCATCTCCGACAAGCTGTGCCTGTCGCCGAAGACCGTGAATACCTATCGCTACCG
CATCTTCGAGAAGCTCTCGATCACCAGCGACGTGGAACTGGCGCTGCTCGCCGTCCGCCACGGCATGGTCGATGCCGCCA
GCTAG