Detailed information of regulator
|Regulatory pathway (from literatures)||TCS-RR|
|KEGG pathway (determined by BlastKOALA)||Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Biofilm formation - Escherichia coli (map02026)|
|Related T6SS||T6SS00003 (Type i3)|
|Strain||Pseudomonas aeruginosa PAO1|
|Sequence||Protein sequence (215 a.a.); Nucleotide sequence (645 bp)|
|Description||The promoter activities of GacS and GacA were increased in the ΔprtNΔprtR mutant and western blot assay showed that the protein levels of GacS and GacA were more abundance in the ΔprtNΔprtR mutant compared to ΔprtN strain. Hcp1 were examined in the wild-type PAO1, ΔprtN, ΔprtNΔprtR,ΔprtNΔprtRΔgacA as well as the complemented strains. The deletion of gacA in the ΔprtNΔprtR strain restored the expression levels of ExoS to those in the ΔprtN mutant and wild-type parent but more fewer for Hcp1.|
External database links
|Locus tag (Gene)||PA2586 (gacA)|
|Coordinates (Strand)||2925788..2926432 (-)|
|KEGG pathway (determined by BlastKOALA)||map02020; map05111; map02025; map02026|
|Target||Regulation (↓/↑)||Target sequence|
|hcp1 (PA0085) expression and secretion||↓||-|
Pfam domain hit(s) of regulator
|Domain||Pfam ID||E-value||Aligned region|
- Transmembrane helices are predicted using TMHMM 2.0 software.
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
|Prediction||Probability||Cleavage site||Signal peptide sequence|
Protein sequence of regulator: 215 a.a. .
Nucleotide sequence of regulator: 645 bp .