Detailed information of regulator


Summary

Regulator ID REG0040 experimental
Regulator type protein
Regulatory pathway (from literatures) RsmA regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (269 a.a.); Nucleotide sequence (807 bp)
Description RsmA negative regulates H1-T6SS in P.aeruginosa PAO1 through binding a motif (AAGGA) in a stem-loop structure overlapping with the ribosome bind site (RBS) in fhA1 mRNA.
Reference
External database links

Locus tag (Gene) PA0592 (RsmA)
Coordinates (Strand) 650575..651381 (-)
NCBI ID 15595789
RefSeq NC_002516
Uniprot ID RSMA_PSEAE
KEGG ID pae:PA0592
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
FHA (PA0081) ↑ gtgcagtggcggctggagaccaggcgctccgggtccggcaggacccagtcgttgtccgggccccgaccgatggtcagctgtccctggtccagtaccttttccgaacactgaccgggggtcagcttgtggtagctggtgatggtcaatcgcagcggcattgctactccttgcattgccagcgcccggctcttccaggcgctcacccccgtgcccgagggatttcggttcgcatccaggctggcccgacggctttccggcgtttcgtcgcctcgggaagacacatcgggt
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
RrnaAD PF00398.22 3.8e-64 7..261
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-268 MSELYQHRARKRFGQNFLHDAGVIHRILRAIHAREGQRLLEIGPGQGALTEGLLGSGARLDVIELDQDLIPLLKLKFGLESRFSLHQGDALKFDFASLVESGEKLRVVGNLPYNISTPLIFHLLEHAPVIEDMHFMLQKEVVERLAATPGGGDWGRLSIMVQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLTPFAEPPHPARDPKLLERVVREAFNQRRKTLRNTLKPLLSVEDIEAAEVDPTLRPEQLDLAAFVRLANQLAELPGNR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9929 - -


  Protein sequence of regulator: 269 a.a.    .

>REG0040 NC_002516:c651381-650575 [Pseudomonas aeruginosa PAO1]
MSELYQHRARKRFGQNFLHDAGVIHRILRAIHAREGQRLLEIGPGQGALTEGLLGSGARLDVIELDQDLIPLLKLKFGLE
SRFSLHQGDALKFDFASLVESGEKLRVVGNLPYNISTPLIFHLLEHAPVIEDMHFMLQKEVVERLAATPGGGDWGRLSIM
VQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLTPFAEPPHPARDPKLLERVVREAFNQRRKTLRNTLKPLLSVEDIEAAEV
DPTLRPEQLDLAAFVRLANQLAELPGNR*

  Nucleotide sequence of regulator: 807 bp    .

>REG0040 NC_002516:c651381-650575 [Pseudomonas aeruginosa PAO1]
ATGTCCGAGCTTTACCAACACCGCGCGCGCAAGCGCTTCGGCCAGAACTTCCTGCACGATGCCGGGGTGATTCACCGCAT
CCTCCGTGCCATCCATGCCCGCGAGGGTCAGCGCCTGCTGGAGATCGGCCCGGGCCAGGGCGCCCTGACCGAGGGACTGC
TGGGCAGCGGCGCACGCCTGGACGTGATCGAGCTCGACCAGGACCTGATCCCTCTGTTGAAGCTGAAATTCGGCCTCGAG
TCGCGCTTCAGCCTGCACCAGGGCGACGCCCTCAAGTTCGATTTCGCCTCGCTGGTGGAGAGCGGCGAGAAGCTGCGAGT
GGTCGGCAACCTGCCCTACAACATCTCCACGCCACTGATCTTCCATCTGCTGGAGCACGCGCCGGTGATCGAGGACATGC
ACTTCATGCTGCAGAAGGAAGTGGTCGAGCGCCTGGCGGCCACGCCGGGCGGCGGAGACTGGGGCCGGCTCTCGATCATG
GTCCAGTATCACTGTCGGGTGGAGCACCTGTTCAACGTCGGCCCCGGCGCGTTCAACCCGCCGCCCAAGGTCGACTCGGC
CATCGTCCGTCTCACCCCCTTCGCCGAGCCGCCCCATCCGGCACGCGATCCGAAACTGCTCGAACGGGTGGTCCGCGAGG
CCTTCAACCAGCGCCGCAAGACCCTGCGCAACACCCTCAAGCCTCTCCTGAGCGTCGAGGACATCGAGGCCGCAGAGGTC
GATCCGACCCTTCGTCCCGAACAACTCGACCTGGCCGCATTCGTCCGCCTGGCCAACCAGCTGGCCGAGCTGCCAGGCAA
TCGTTAG