Detailed information of effector
Summary
| Effector ID | EFF00014  |
| Effector type | Tae |
| Cognate immunity protein | IMU00190 |
| Related T6SS (Type) | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (155 a.a.); Nucleotide sequence (465 bp) |
| Description | Tse1 (Tae1); peptidoglycan amidase |
| Reference | [1] Hood RD, Singh P, Hsu F, et al. A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe. 2010 Jan 21;7(1):25-37. doi: 10.1016/j.chom.2009.12.007. PMID: 20114026 [2] Russell AB, Hood RD, Bui NK, et al. Type VI secretion delivers bacteriolytic effectors to target cells. Nature. 2011 Jul 20;475(7356):343-7. doi: 10.1038/nature10244. PMID: 21776080 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA1844 (Tse1, Tae1) |
| Coordinates (Strand) | 2003868..2004332 (-) |
| NCBI ID | 15597041 |
| RefSeq | NC_002516 |
| Uniprot ID | TSE1_PSEAE |
| KEGG ID | pae:PA1844 |
| PDB ID | 4FQB, 3VPI, 4FGD, 4FQA, 4EQ8, 4F0V, 4FGE, 3VPJ, 4EOB, 4F0W, 4FGI, 4F4M, 4EQA |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-154 | MDSLDQCIVNACKNSWDKSYLAGTPNKDNCSGFVQSVAAELGVPMPRGNANAMVDGLEQSWTKLASGAEAAQKAAQGFLVIAGLKGRTYGHVAVVISGPLYRQKYPMCWCGSIAGAVGQSQGLKSVGQVWNRTDRDRLNYYVYSLASCSLPRAS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9793 | - | - |
Protein sequence: 155 a.a. .
>EFF00014 NC_002516:c2004332-2003868 Type VI Amidase Effector (Tae) [Pseudomonas aeruginosa PAO1]
MDSLDQCIVNACKNSWDKSYLAGTPNKDNCSGFVQSVAAELGVPMPRGNANAMVDGLEQSWTKLASGAEAAQKAAQGFLV
IAGLKGRTYGHVAVVISGPLYRQKYPMCWCGSIAGAVGQSQGLKSVGQVWNRTDRDRLNYYVYSLASCSLPRAS*
MDSLDQCIVNACKNSWDKSYLAGTPNKDNCSGFVQSVAAELGVPMPRGNANAMVDGLEQSWTKLASGAEAAQKAAQGFLV
IAGLKGRTYGHVAVVISGPLYRQKYPMCWCGSIAGAVGQSQGLKSVGQVWNRTDRDRLNYYVYSLASCSLPRAS*
Nucleotide sequence: 465 bp .
>EFF00014 NC_002516:c2004332-2003868 Type VI Amidase Effector (Tae) [Pseudomonas aeruginosa PAO1]
ATGGACAGTCTCGATCAATGCATCGTCAACGCCTGCAAGAACAGCTGGGACAAGAGCTACCTGGCCGGCACCCCGAACAA
GGACAACTGTTCCGGCTTCGTCCAGTCGGTGGCCGCCGAGCTGGGCGTACCGATGCCCCGCGGCAACGCCAACGCCATGG
TCGACGGCCTGGAGCAGAGCTGGACCAAGCTCGCCTCCGGCGCCGAGGCCGCGCAGAAGGCGGCCCAGGGCTTCCTGGTG
ATCGCCGGCCTGAAGGGCCGCACCTACGGGCACGTCGCGGTGGTCATCAGCGGTCCGCTGTATCGGCAGAAGTACCCGAT
GTGCTGGTGCGGCAGCATCGCCGGCGCGGTCGGCCAGAGCCAGGGCCTGAAGTCGGTCGGCCAGGTGTGGAATCGCACCG
ACCGCGACCGCCTCAACTACTACGTCTACTCCCTGGCCAGTTGCAGCCTGCCCAGGGCCAGTTGA
ATGGACAGTCTCGATCAATGCATCGTCAACGCCTGCAAGAACAGCTGGGACAAGAGCTACCTGGCCGGCACCCCGAACAA
GGACAACTGTTCCGGCTTCGTCCAGTCGGTGGCCGCCGAGCTGGGCGTACCGATGCCCCGCGGCAACGCCAACGCCATGG
TCGACGGCCTGGAGCAGAGCTGGACCAAGCTCGCCTCCGGCGCCGAGGCCGCGCAGAAGGCGGCCCAGGGCTTCCTGGTG
ATCGCCGGCCTGAAGGGCCGCACCTACGGGCACGTCGCGGTGGTCATCAGCGGTCCGCTGTATCGGCAGAAGTACCCGAT
GTGCTGGTGCGGCAGCATCGCCGGCGCGGTCGGCCAGAGCCAGGGCCTGAAGTCGGTCGGCCAGGTGTGGAATCGCACCG
ACCGCGACCGCCTCAACTACTACGTCTACTCCCTGGCCAGTTGCAGCCTGCCCAGGGCCAGTTGA