Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00190  |
| Cognate effector | EFF00014 |
| Related T6SS (Type) | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (173 a.a.); Nucleotide sequence (519 bp) |
| Description | Tsi1 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA1845 (Tsi1,Tai1) |
| Coordinates (Strand) | 2004374..2004892 (-) |
| NCBI ID | 15597042 |
| RefSeq | NC_002516 |
| Uniprot ID | TSI1_PSEAE |
| KEGG ID | pae:PA1845 |
| PDB ID | 4EQA, 4FQB, 4FGI, 3VPJ |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-172 | MKLLAGSFAALFLSLSAQAADCTFTQLEIVPQFGSPNMFGGEDEHVRVMFSNEDPNDDNPDAFPEPPVYLADRDSGNDCRIEDGGIWSRGGVFLSQDGRRVLMHEFSGSSAELVSYDSATCKVVHREDISGQRWAVDKDGLRLGQKCSGESVDSCAKIVKRSLAPFCQTAKK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9976 | pos: 19-20. AQA-AD. | MKLLAGSFAALFLSLSAQA |
Protein sequence: 173 a.a. .
>IMU00190 NC_002516:c2004892-2004374 [Pseudomonas aeruginosa PAO1]
MKLLAGSFAALFLSLSAQAADCTFTQLEIVPQFGSPNMFGGEDEHVRVMFSNEDPNDDNPDAFPEPPVYLADRDSGNDCR
IEDGGIWSRGGVFLSQDGRRVLMHEFSGSSAELVSYDSATCKVVHREDISGQRWAVDKDGLRLGQKCSGESVDSCAKIVK
RSLAPFCQTAKK*
MKLLAGSFAALFLSLSAQAADCTFTQLEIVPQFGSPNMFGGEDEHVRVMFSNEDPNDDNPDAFPEPPVYLADRDSGNDCR
IEDGGIWSRGGVFLSQDGRRVLMHEFSGSSAELVSYDSATCKVVHREDISGQRWAVDKDGLRLGQKCSGESVDSCAKIVK
RSLAPFCQTAKK*
Nucleotide sequence: 519 bp .
>IMU00190 NC_002516:c2004892-2004374 [Pseudomonas aeruginosa PAO1]
ATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAGCCTTTCGGCCCAGGCGGCGGACTGTACTTTCACCCAGCT
GGAAATCGTGCCCCAGTTCGGCTCGCCGAACATGTTCGGCGGCGAGGACGAGCACGTCCGCGTGATGTTCAGCAACGAGG
ATCCCAACGACGACAACCCCGATGCCTTTCCCGAACCGCCGGTCTACCTGGCCGACCGCGACAGCGGCAACGATTGCCGC
ATAGAGGACGGCGGCATCTGGTCGCGCGGCGGCGTGTTCCTGAGCCAGGACGGCCGGCGCGTGCTGATGCATGAATTCAG
CGGCTCCAGCGCCGAGCTGGTGTCCTACGACAGCGCCACCTGCAAGGTGGTGCACCGCGAGGACATCTCCGGCCAGCGCT
GGGCGGTGGACAAGGACGGGCTGCGCCTGGGTCAGAAATGCTCGGGCGAATCCGTCGACAGCTGCGCGAAGATCGTGAAG
CGTTCGCTCGCGCCGTTCTGCCAGACCGCAAAGAAATGA
ATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAGCCTTTCGGCCCAGGCGGCGGACTGTACTTTCACCCAGCT
GGAAATCGTGCCCCAGTTCGGCTCGCCGAACATGTTCGGCGGCGAGGACGAGCACGTCCGCGTGATGTTCAGCAACGAGG
ATCCCAACGACGACAACCCCGATGCCTTTCCCGAACCGCCGGTCTACCTGGCCGACCGCGACAGCGGCAACGATTGCCGC
ATAGAGGACGGCGGCATCTGGTCGCGCGGCGGCGTGTTCCTGAGCCAGGACGGCCGGCGCGTGCTGATGCATGAATTCAG
CGGCTCCAGCGCCGAGCTGGTGTCCTACGACAGCGCCACCTGCAAGGTGGTGCACCGCGAGGACATCTCCGGCCAGCGCT
GGGCGGTGGACAAGGACGGGCTGCGCCTGGGTCAGAAATGCTCGGGCGAATCCGTCGACAGCTGCGCGAAGATCGTGAAG
CGTTCGCTCGCGCCGTTCTGCCAGACCGCAAAGAAATGA