Detailed information of effector
|Cognate immunity protein||IMU00382|
|Related T6SS (Type)||T6SS00004 (Type i1)|
|Strain||Pseudomonas aeruginosa PAO1|
|Sequence||Protein sequence (149 a.a.); Nucleotide sequence (447 bp)|
|Description||The PA0823 fused PA0822 gene was generated by mutate the stop codon TAA of PA0823 gene into TAC from the P. aeruginosa PAO1 genomic DNA thus generating a sequence encoding a 233AA protein from the N terminal of PA0823 to the C-terminal of TseV. in vivo Escherichia coli toxicity assay further supported this deduction. Isopropyl b-D- 1-thiogalactopyranoside (IPTG) induced expression of TseV substantially reduced the growth of E. coli cells and the toxicity of TseV could be rescued by co-expression with TsiV.|
Bioinformatics investigation has been performed
External database links
|Locus tag (Gene)||PA0822 (TseV)|
|Coordinates (Strand)||898440..898886 (-)|
Pfam domain hit(s)
|Domain||Pfam ID||E-value||Aligned region|
- Transmembrane helices are predicted using TMHMM 2.0 software.
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
|Prediction||Probability||Cleavage site||Signal peptide sequence|
Protein sequence: 149 a.a. .
Nucleotide sequence: 447 bp .