Detailed information of effector
Summary
| Effector ID | EFF01891  |
| Effector type | Tne |
| Cognate immunity protein | IMU00382 |
| Related T6SS (Type) | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (149 a.a.); Nucleotide sequence (447 bp) |
| Description | The PA0823 fused PA0822 gene was generated by mutate the stop codon TAA of PA0823 gene into TAC from the P. aeruginosa PAO1 genomic DNA thus generating a sequence encoding a 233AA protein from the N terminal of PA0823 to the C-terminal of TseV. in vivo Escherichia coli toxicity assay further supported this deduction. Isopropyl b-D- 1-thiogalactopyranoside (IPTG) induced expression of TseV substantially reduced the growth of E. coli cells and the toxicity of TseV could be rescued by co-expression with TsiV. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA0822 (TseV) |
| Coordinates (Strand) | 898440..898886 (-) |
| NCBI ID | - |
| RefSeq | NC_002516 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| VRR_NUC | PF08774.13 | 1e-06 | 43..93 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-148 | MTKNPPQPILDSQTGNSPHGWIPGWISKYWDEDPEHPPFKPGKGMIRRPDVIIVQNPNRPPTQDNIKQVVEMKFPPDPHNREQLEDYAAIAGNKNKIVEMKPSDCDCGQENQRSKVPVEQAGWAVAIAGGVMFVLTRGRSPRPMIPAY |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9824 | - | - |
Protein sequence: 149 a.a. .
>EFF01891 NC_002516:c898886-898440 Type VI NADase Effector (Tne) [Pseudomonas aeruginosa PAO1]
MTKNPPQPILDSQTGNSPHGWIPGWISKYWDEDPEHPPFKPGKGMIRRPDVIIVQNPNRPPTQDNIKQVVEMKFPPDPHN
REQLEDYAAIAGNKNKIVEMKPSDCDCGQENQRSKVPVEQAGWAVAIAGGVMFVLTRGRSPRPMIPAY*
MTKNPPQPILDSQTGNSPHGWIPGWISKYWDEDPEHPPFKPGKGMIRRPDVIIVQNPNRPPTQDNIKQVVEMKFPPDPHN
REQLEDYAAIAGNKNKIVEMKPSDCDCGQENQRSKVPVEQAGWAVAIAGGVMFVLTRGRSPRPMIPAY*
Nucleotide sequence: 447 bp .
>EFF01891 NC_002516:c898886-898440 Type VI NADase Effector (Tne) [Pseudomonas aeruginosa PAO1]
ATGACCAAGAACCCACCGCAGCCGATTCTCGATAGCCAGACCGGTAACAGTCCACATGGCTGGATACCGGGATGGATCAG
CAAGTATTGGGATGAAGATCCGGAGCATCCGCCGTTCAAGCCAGGGAAAGGAATGATTCGCAGGCCCGACGTGATCATCG
TTCAGAATCCCAACAGGCCGCCAACCCAGGACAATATCAAGCAGGTGGTGGAGATGAAGTTTCCGCCTGACCCACATAAT
AGGGAACAACTTGAGGATTATGCAGCGATTGCCGGCAACAAGAACAAGATTGTTGAAATGAAACCCTCCGACTGCGACTG
TGGCCAGGAAAATCAACGATCCAAGGTGCCAGTAGAGCAAGCAGGGTGGGCCGTCGCCATCGCAGGTGGAGTGATGTTCG
TCTTGACCCGAGGCCGTTCCCCTCGCCCGATGATTCCCGCCTATTGA
ATGACCAAGAACCCACCGCAGCCGATTCTCGATAGCCAGACCGGTAACAGTCCACATGGCTGGATACCGGGATGGATCAG
CAAGTATTGGGATGAAGATCCGGAGCATCCGCCGTTCAAGCCAGGGAAAGGAATGATTCGCAGGCCCGACGTGATCATCG
TTCAGAATCCCAACAGGCCGCCAACCCAGGACAATATCAAGCAGGTGGTGGAGATGAAGTTTCCGCCTGACCCACATAAT
AGGGAACAACTTGAGGATTATGCAGCGATTGCCGGCAACAAGAACAAGATTGTTGAAATGAAACCCTCCGACTGCGACTG
TGGCCAGGAAAATCAACGATCCAAGGTGCCAGTAGAGCAAGCAGGGTGGGCCGTCGCCATCGCAGGTGGAGTGATGTTCG
TCTTGACCCGAGGCCGTTCCCCTCGCCCGATGATTCCCGCCTATTGA