Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00004 experimental
T6SS type Type i1
Strain Pseudomonas aeruginosa PAO1
Replicon (RefSeq) chromosome (H2-T6SS) (NC_002516)
Location 1797958..1827821
Function anti-eukaryotic; anti-bacterial; effector translocation; virulence
Comment H2-T6SS modulates internalization in epithelial cells; virulence in the worm model; virulence (slow killing) in Caenorhabditis elegans model; PldA achieves its antibacterial activity by degrading phosphatidylethanolamine; PldA contributes to the internalization of P. aeruginosa into Human Epithelial Cells and activates the PI3K/Akt Pathway during P. aeruginosa Infection.
References
[1] Sana TG et al (2012) The Second Type VI Secretion System of Pseudomonas aeruginosa Strain PAO1 Is Regulated by Quorum Sensing and Fur and Modulates Internalization in Epithelial Cells. J Biol Chem. 287(32):27095-105. [PudMed:22665491]
[2] Osipiuk J et al (2011) Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
[3] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
[4] Hachani A et al (2011) Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275]
[5] Bleves S et al (2010) Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
[6] Russell AB et al (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891]
[7] Sana TG et al (2013) Divergent Control of Two Type VI Secretion Systems by RpoN in Pseudomonas aeruginosa. PLoS One. 8(10):e76030. [PudMed:24204589]
[8] Jiang F et al (2014) A Pseudomonas aeruginosa Type VI Secretion Phospholipase D Effector Targets Both Prokaryotic and Eukaryotic Cells. Cell Host Microbe. 15(5):600-10. [PudMed:24832454]
[9] Sana TG et al (2015) Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network. MBio. 6(3):e00712. [PudMed:26037124]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHASfa2Stk1Stp1TssATssBTssCTssETssFTssGTssHTssJTssKTssLTssM
Number111111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
PA1647 1793019..1794740 - 1722 NP_250338.1 sulfate transporter
PA1648 1794883..1795887 - 1005 NP_250339.1 oxidoreductase
PA1649 1796138..1796899 + 762 NP_250340.1 short-chain dehydrogenase
PA1650 1797058..1797951 + 894 NP_250341.1 transporter
PA1651 1797958..1799154 - 1197 NP_250342.1 transporter
PA1652 1799385..1800020 + 636 NP_250343.1 hypothetical protein
PA1653 1800071..1800547 - 477 NP_250344.1 transcriptional regulator
PA1654 1800629..1801795 + 1167 NP_250345.1 aminotransferase
PA1655 1801851..1802453 + 603 NP_250346.1 glutathione S-transferase
T6CP008377 TssA PA1656 1803626..1805182 + 1557 NP_250347.1 hypothetical protein
T6CP000011 TssB PA1657 1805218..1805724 + 507 NP_250348.1 hypothetical protein
T6CP000012 TssC PA1658 1805753..1807228 + 1476 NP_250349.1 hypothetical protein
T6CP008378 TssE PA1659 1807241..1807648 + 408 NP_250350.1 hypothetical protein
T6CP000013 TssF PA1660 1808193..1809773 + 1581 NP_250351.1 hypothetical protein
T6CP000014 TssG PA1661 1809737..1810744 + 1008 NP_250352.1 hypothetical protein
T6CP000015 TssH PA1662 1810751..1813384 + 2634 NP_250353.1 ClpA/B-type protease
T6CP012421 Sfa2 PA1663 1813395..1814906 + 1512 NP_250354.1 transcriptional regulator
PA1664 1814995..1815135 + 141 NP_250355.1 hypothetical protein
T6CP011362 FHA PA1665 1815153..1816346 + 1194 NP_250356.1 hypothetical protein
T6CP000016 TssJ PA1666 1816352..1816858 + 507 NP_250357.1 hypothetical protein
T6CP000017 TssK PA1667 1816855..1818186 + 1332 NP_250358.1 hypothetical protein
T6CP000018 TssL PA1668 1818189..1819058 + 870 NP_250359.1 hypothetical protein
T6CP000019 TssM PA1669 1819074..1822601 + 3528 NP_250360.1 hypothetical protein
T6CP012422 Stp1 PA1670 (stp1) 1822601..1823329 + 729 NP_250361.1 serine/threonine phosphoprotein phosphatase Stp1
T6CP012423 Stk1 PA1671 (stk1) 1823326..1824315 + 990 NP_250362.1 serine-threonine kinase Stk1
PA1672 1824343..1824723 - 381 NP_250363.1 hypothetical protein
PA1673 1824969..1825430 + 462 NP_250364.1 bacteriohemerythrin
PA1674 (folE2) 1825495..1826040 - 546 NP_250365.1 GTP cyclohydrolase I
PA1675 1826118..1826675 - 558 NP_250366.1 hypothetical protein
PA1676 1826732..1827052 - 321 NP_250367.1 hypothetical protein
PA1677 1827225..1827821 - 597 NP_250368.1 hypothetical protein
PA1678 1827992..1828906 + 915 NP_250369.1 50S ribosomal protein L3 glutamine methyltransferase
PA1679 1828931..1829710 - 780 NP_250370.1 hypothetical protein
PA1680 1829788..1830771 - 984 NP_250371.1 hypothetical protein
PA1681 (aroC) 1830879..1831970 + 1092 NP_250372.1 chorismate synthase
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0018 experimental PA4462 (RpoN) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4992870..4994363 (+) 15599658 PA1512 (hcpA) promotor (↓); PA5267 (hcpB) promotor (↓)
REG0059 experimental PA4704.1 (prrF1) ncRNA QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5283960..5284110 (+) 4179187 T6SS transcripts (↓)
REG0060 experimental PA4704.2 (prrF2) ncRNA QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5284172..5284319 (+) 4179185 T6SS transcripts (↓)
REG0061 experimental PA0996 (pqsA) protein Unknown Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1078462..1080015 (+) 15596193 T6SS transcripts
REG0062 experimental PA1244 (QslA) protein QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1348060..1348401 (-) 15596441 H2-T6SS (PA1656) promotor (↑)
REG0063 experimental PA4778 (CueR) protein Transcription regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5366257..5366655 (+) 15599972 tssA2 (-173 to -140 relative to the ATG start codon) (↓); hcp2 (-208 to -159 relative to the ATG start codon) (↓)
REG0065 experimental PA1430 (LasR) protein Global regulator; QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1558171..1558890 (+) 15596627 H2-T6SS promotor P (hsiA2) (↓)
REG0066 experimental PA3476 (rhlI) protein QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 3889139..3889744 (-) 15598672 PA3477 (RhlR) (↓)
REG0067 experimental PA3477 (RhlR) protein QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 3889925..3890650 (-) 15598673 T6SS transcript (↓)
REG0068 experimental PA4764 (Fur) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5351675..5352079 (-) 15599958 H2-T6SS Transcription (↑)
REG0069 experimental PA4462 (rpoN) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4992870..4994363 (+) 15599658 T6SS transcript (↑)
REG0070 experimental pa1663 (sfa2) protein Global regulator; QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1813395..1814906 (+) 15596860 PA4462 (rpoN) (↑); T6SS transcript (↑); T6SS transcript (↓)
REG0368 experimental PA1544 (Anr) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1681071..1681805 (-) 15596741 H2-T6SS transcriptional level and ModA secretion (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00080 experimental PA3488 (Tli5) EFF00019 experimental Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 3906391..3907089 (+) 15598684
IMU00314 experimental PA1509 (TplEi) EFF01463 experimental Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1638652..1639794 (-) 15596706
IMU00315 experimental PA0261 (Tli3) EFF01450; EFF01496 insolico; EFF01450; EFF01496 insolico Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 293301..293798 (-) 15595458

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00005 experimental PA0259 (tla3) adaptor Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 289562..291004 (-) 15595456 PA0262 (VgrG2b) PA0260 (Tle3)
ACP00043 experimental PA4370 (IcmP) chaperone Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4898193..4899533 (+) 15599566 - PA1863 (ModA)
ACP00044 experimental PA1862 (ModB) chaperone Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 2021712..2022398 (-) 15597059 - PA1863 (ModA)
ACP00045 experimental PA1861 (ModC) chaperone Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 2020625..2021710 (-) 15597058 - PA1863 (ModA)