Detailed information of regulator


Summary

Regulator ID REG0061 experimental
Regulator type protein
Regulatory pathway (from literatures) Unknown
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00004 experimental (Type i1)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (518 a.a.); Nucleotide sequence (1554 bp)
Description The ∆pqsA mutant demonstrated even more dramatic decreases in the expression of all three genes relative to wild type PAO1 than did the ∆prrF mutantduring static growth, supporting the notion that AQs are necessary for the expression of T6SS in static growth.AQ-dependent expression of several other T6SS genes was also observedin static cultures. In contrast,pqsA was not required for T6SS gene expressionin shaking conditions,supporting the hypothesis that AQ-dependent activation of T6SS is enhanced by static growth.
Reference
External database links

Locus tag (Gene) PA0996 (pqsA)
Coordinates (Strand) 1078462..1080015 (+)
NCBI ID 15596193
RefSeq NC_002516
Uniprot ID PQSA_PSEAE
KEGG ID pae:PA0996
KEGG pathway (determined by BlastKOALA) -
PDB ID 5OE3, 5OE4, 5OE6, 5OE5
  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
AMP-binding PF00501.30 4.5e-72 18..404
AMP-binding_C PF13193.8 2.4e-08 414..493
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-517 MSTLANLTEVLFRLDFDPDTAVYHYRGQTLSRLQCRTYILSQASQLARLLKPGDRVVLALNDSPSLACLFLACIAVGAIPAVINPKSREQALADIAADCQASLVVREADAPSLSGPLAPLTLRAAAGRPLLDDFSLDALVGPADLDWSAFHRQDPAAACFLQYTSGSTGAPKGVMHSLRNTLGFCRAFATELLALQAGDRLYSIPKMFFGYGMGNSLFFPWFSGASALLDDTWPSPERVLENLVAFRPRVLFGVPAIYASLRPQARELLSSVRLAFSAGSPLPRGEFEFWAAHGLEICDGIGATEVGHVFLANRPGQARADSTGLPLPGYECRLVDREGHTIEEAGRQGVLLVRGPGLSPGYWRASEEQQARFAGGWYRTGDLFERDESGAYRHCGREDDLFKVNGRWVVPTQVEQAICRHLPEVSEAVLVPTCRLHDGLRPTLFVTLATPLDDNQILLAQRIDQHLAEQIPSHMLPSQLHVLPALPRNDNGKLARAELRHLADTLYHDNLPEERAC


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9970 - -


  Protein sequence of regulator: 518 a.a.    .

>REG0061 NC_002516:1078462-1080015 [Pseudomonas aeruginosa PAO1]
MSTLANLTEVLFRLDFDPDTAVYHYRGQTLSRLQCRTYILSQASQLARLLKPGDRVVLALNDSPSLACLFLACIAVGAIP
AVINPKSREQALADIAADCQASLVVREADAPSLSGPLAPLTLRAAAGRPLLDDFSLDALVGPADLDWSAFHRQDPAAACF
LQYTSGSTGAPKGVMHSLRNTLGFCRAFATELLALQAGDRLYSIPKMFFGYGMGNSLFFPWFSGASALLDDTWPSPERVL
ENLVAFRPRVLFGVPAIYASLRPQARELLSSVRLAFSAGSPLPRGEFEFWAAHGLEICDGIGATEVGHVFLANRPGQARA
DSTGLPLPGYECRLVDREGHTIEEAGRQGVLLVRGPGLSPGYWRASEEQQARFAGGWYRTGDLFERDESGAYRHCGREDD
LFKVNGRWVVPTQVEQAICRHLPEVSEAVLVPTCRLHDGLRPTLFVTLATPLDDNQILLAQRIDQHLAEQIPSHMLPSQL
HVLPALPRNDNGKLARAELRHLADTLYHDNLPEERAC*

  Nucleotide sequence of regulator: 1554 bp    .

>REG0061 NC_002516:1078462-1080015 [Pseudomonas aeruginosa PAO1]
ATGTCCACATTGGCCAACCTGACCGAGGTTCTGTTCCGCCTCGATTTCGATCCCGATACCGCCGTTTATCACTATCGGGG
CCAGACTCTCAGCCGGCTGCAATGCCGGACCTACATTCTCTCCCAGGCCAGCCAACTGGCCCGCCTGCTCAAGCCCGGCG
ATCGCGTGGTGCTGGCGTTGAACGACTCGCCTTCGCTGGCCTGCCTGTTCCTGGCCTGCATCGCGGTCGGCGCCATTCCC
GCCGTGATCAATCCCAAGTCCCGCGAGCAGGCCCTGGCCGATATCGCTGCCGACTGCCAGGCCAGCCTGGTGGTGCGTGA
AGCCGATGCACCGTCGCTGAGCGGTCCTTTGGCGCCGTTGACCCTGCGTGCGGCCGCCGGACGCCCTTTGCTCGACGATT
TCTCGCTGGACGCGCTGGTCGGCCCTGCGGACCTCGATTGGAGTGCCTTCCATCGCCAGGACCCGGCGGCAGCCTGTTTC
CTGCAATACACCTCGGGTTCCACCGGGGCGCCCAAGGGGGTGATGCACAGCCTGCGCAACACGCTCGGTTTCTGCCGGGC
GTTCGCTACGGAGTTGCTGGCATTGCAGGCGGGAGACCGGCTGTATTCGATTCCCAAGATGTTCTTCGGCTATGGCATGG
GCAACAGCCTGTTCTTTCCCTGGTTCAGCGGAGCCTCGGCGCTGCTCGACGATACCTGGCCGAGCCCGGAGCGGGTTCTG
GAGAACCTGGTCGCCTTCCGCCCCCGGGTCCTGTTTGGGGTGCCGGCCATCTATGCCTCGCTGCGTCCGCAGGCCAGGGA
GCTGTTGAGCAGCGTGCGCCTGGCGTTTTCCGCCGGCTCGCCGCTGCCGCGCGGCGAGTTCGAATTCTGGGCCGCGCACG
GGCTGGAGATCTGCGACGGCATCGGGGCTACCGAGGTCGGCCATGTGTTCCTCGCCAACCGCCCGGGCCAGGCGCGTGCC
GACAGCACCGGGCTGCCGTTGCCTGGCTATGAGTGCCGGCTGGTGGACCGCGAAGGACACACTATCGAGGAAGCGGGCCG
GCAAGGCGTGCTGTTGGTGCGTGGCCCAGGGCTGAGTCCGGGTTACTGGCGGGCCAGCGAAGAGCAGCAGGCGCGCTTCG
CAGGTGGCTGGTACCGCACCGGCGACCTGTTCGAGCGCGACGAGTCGGGTGCCTACCGTCACTGTGGGCGGGAAGACGAT
CTGTTCAAGGTGAATGGCCGCTGGGTGGTGCCGACCCAGGTCGAGCAGGCGATCTGCCGTCATCTGCCGGAAGTGAGCGA
GGCGGTTCTGGTTCCTACCTGCCGGCTGCACGACGGCTTGCGTCCGACCCTGTTCGTCACCCTGGCCACTCCGCTGGACG
ACAACCAGATCCTGCTGGCGCAGCGCATCGACCAGCATCTCGCCGAACAGATTCCCTCGCACATGCTGCCCAGCCAATTG
CATGTGCTGCCGGCCTTGCCGCGCAACGACAACGGCAAGTTGGCGCGCGCCGAGCTGCGCCACCTGGCCGACACCCTTTA
TCACGACAACCTTCCGGAGGAACGGGCATGTTGA