Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0020	Qrr4	T6SS01061	ncRNA	Unknown	Vibrio cholerae 2740-80	chromosome (NZ_CP016325)	-	-	VCA0107 mRNA (↑)
REG0021	GPY04_RS11195 (CytR)	T6SS01168	protein	Unknown	Vibrio cholerae C6706	chromosome (NZ_CP046844)	2449479..2450486 (-)	446146597	Unknown (↓)
REG0036	VC1920/VCA1552_01695 (LonA)	T6SS01112	protein	Unknown	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	1845472..1847832 (-)	446970355	Unknown (↑)
REG0050	PA2492 (MexT)	T6SS00003	protein	Unknown	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	2807469..2808512 (+)	15597688	T6SS transcripts (↑)
REG0051	PA1006	T6SS00003	protein	Unknown	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	1090606..1090857 (+)	15596203	T6SS transcripts (↓)
REG0055	PA4109 (AmpR)	T6SS00003	protein	Unknown	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	4592990..4593880 (-)	15599304	T6SS transcripts (↑)
REG0058	PA2976 (RNase E)	T6SS00003	protein	Unknown	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	3332881..3336054 (+)	15598172	PA0077 (icmF1) expression (↑)
REG0061	PA0996 (pqsA)	T6SS00004	protein	Unknown	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	1078462..1080015 (+)	15596193	T6SS transcripts
REG0089	xac4129 (ecfK/RpoE,ECF σ factor)	T6SS00028	protein	Unknown	Xanthomonas citri pv. citri str. 306	chromosome (NC_003919)	4850278..4850805 (+)	489582896	Unknown (↓)
REG0090	xac4127 (PknS)	T6SS00028	protein	Unknown	Xanthomonas citri pv. citri str. 306	chromosome (NC_003919)	4846892..4849411 (-)	505284326	Unknown (↓)
REG0106	vp2049 (Thymidylate kinase, Tmk)	T6SS00043	protein	Unknown	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2150113..2150745 (-)	28898823	T6SS1 (VgrG1) expression and secretion (↓)
REG0109	vp1407	T6SS00043	protein	Unknown	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	1511565..1512017 (-)	28898181	T6SS1 (VgrG1) expression and secretion (↓)
REG0131	BPSL3431 (TctR)	T6SS00074	protein	Unknown	Burkholderia pseudomallei K96243	chromosome (NC_006350)	4073388..4074029 (-)	53721038	T6SS-2 (hcp2) transcript (↑)
REG0132	BPSL2246 (PpiB)	T6SS00076 ; T6SS00077	protein	Unknown	Burkholderia pseudomallei K96243	chromosome (NC_006350)	2708622..2709113 (+)	53719855	T6SS-3 (BPSS2099/TssCIII and BPSS2098/HcpIII) expression (↑); T6SS-6 (hcp6) transcript (↓)
REG0133	BPSS1504	T6SS00077	protein	Unknown	Burkholderia pseudomallei K96243	chromosome (NC_006351)	2052905..2055547 (+)	499518990	hcp1 secretion
REG0186	YpsIP31758_3441 (lptD)	T6SS00225	protein	Unknown	Yersinia pseudotuberculosis IP 31758	chromosome (NC_009708)	3878499..3880841 (+)	499505072	YpsIP31758_3980 (ompR) (↓)
REG0202	PXO_RS20535	T6SS00287	protein	Unknown	Xanthomonas oryzae pv. oryzae PXO99A	chromosome (NC_010717)	4488457..4488843 (-)	499576999	Unknown (↓)
REG0371	VFES401_RS15770 (tasR)	T6SS17506	protein	Unknown	Aliivibrio fischeri strain ES401	chromosome (NZ_SRJG01000005)	373862..374311 (+)	WP_012535053.1	hcp promotor (↓)