Detailed information of regulator


Summary

Regulator ID REG0133 experimental
Regulator type protein
Regulatory pathway (from literatures) Unknown
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00077 insolico (Type i4b)
Strain Burkholderia pseudomallei K96243
Replicon chromosome
Sequence Protein sequence (881 a.a.); Nucleotide sequence (2643 bp)
Description Lack of BPSS1504 in B. pseudomallei clearly resulted in classical T6SS-associated phenotypes that are similar to that of a BpΔhcp1 mutant and Hcp1 was detectable in cells after infection with WT bacteria and the BpΔBPSS1504::BPSS1504 complemented strain at an MOI of 2.
Reference
External database links

Locus tag (Gene) BPSS1504
Coordinates (Strand) 2052905..2055547 (+)
NCBI ID 499518990
RefSeq NC_006351
Uniprot ID Q63K61_BURPS
KEGG ID bps:BPSS1504
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
hcp1 secretion -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
DUF2169 PF09937.11 1.4e-25 122..240
Pentapeptide_4 PF13599.8 4.8e-09 577..652
Pentapeptide PF00805.24 9.3e-09 796..835
Pentapeptide PF00805.24 4.1e-08 752..788
DUF2169 PF09937.11 1.5e-05 22..105
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-880 MKIVKPETLALMCRTLRIERADRLSIGALACFALRAHAPDGPGDLAPEATLWQIAQQWLGAHAPLDEGWPKPAGEFLVYGDACAPAGREHAGGAPFAVRARIGAACKARLVDARDPADRVLADFRALPPSHPQRVRDLGPFDARWLAERWPHLPSGTRAEHFHTAPRDQRIAGFWRGDEDIELVNLHAQHPIVAGALPRVRARCFVERSAGGATRVDACPMRAETVWLFPGAACGIVLYRGLATIDDEDGDDVLRVIAGWEDAAAPPLPADAYLGRPASGGAGSRPTPAPDAAPVASVVPAALTAEEAHADEHAPGGSASASQAHSPAAPEFPEAPHAPDLSALEREAAALAGQTDALLAGLGITEADIARLLPARDAPAELNLDQLATLAAELDAQTAQWQAQQAAAAVERGDAASATPAAPATPDAEAAHEASLADLLRQADAQMRALVEQHGLSRARMEAAARTLPELAPLAGSLDALDALDAPLDVDALTAGLAAAGGDAAAEPDTPAEPSPPAPANEFAAAVPAPASSTAAPPVDDAPPGPLTREQVIERHARGLGFAGLDLSGLDLSSAALERADFRRARLERTRFAGCRLAGASFERALLSHADFSNADLRDAVFAGASAPGASWRGAVLERARLEHGDFSGGDFAQASLADSHCAHAQFDASAMTALVAARIDGTHASFAGCTLDAADFTSARLPRANFQHATLADAALACAHCDGAEWYGAQAPRARLRAASLRGSRADASTSFRQADLSSAALDDANWDGVDLRGTNLHEATLDGASLARANASGAQLTRARARRADLTQADLTHADARCSNLHGASLRRARLGGTQLQSSNLYGADCYGTALARPQLDGANIERTLLAVPGRPELAASR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.5613 - -


  Protein sequence of regulator: 881 a.a.    .

>REG0133 NC_006351:2052905-2055547 [Burkholderia pseudomallei K96243]
MKIVKPETLALMCRTLRIERADRLSIGALACFALRAHAPDGPGDLAPEATLWQIAQQWLGAHAPLDEGWPKPAGEFLVYG
DACAPAGREHAGGAPFAVRARIGAACKARLVDARDPADRVLADFRALPPSHPQRVRDLGPFDARWLAERWPHLPSGTRAE
HFHTAPRDQRIAGFWRGDEDIELVNLHAQHPIVAGALPRVRARCFVERSAGGATRVDACPMRAETVWLFPGAACGIVLYR
GLATIDDEDGDDVLRVIAGWEDAAAPPLPADAYLGRPASGGAGSRPTPAPDAAPVASVVPAALTAEEAHADEHAPGGSAS
ASQAHSPAAPEFPEAPHAPDLSALEREAAALAGQTDALLAGLGITEADIARLLPARDAPAELNLDQLATLAAELDAQTAQ
WQAQQAAAAVERGDAASATPAAPATPDAEAAHEASLADLLRQADAQMRALVEQHGLSRARMEAAARTLPELAPLAGSLDA
LDALDAPLDVDALTAGLAAAGGDAAAEPDTPAEPSPPAPANEFAAAVPAPASSTAAPPVDDAPPGPLTREQVIERHARGL
GFAGLDLSGLDLSSAALERADFRRARLERTRFAGCRLAGASFERALLSHADFSNADLRDAVFAGASAPGASWRGAVLERA
RLEHGDFSGGDFAQASLADSHCAHAQFDASAMTALVAARIDGTHASFAGCTLDAADFTSARLPRANFQHATLADAALACA
HCDGAEWYGAQAPRARLRAASLRGSRADASTSFRQADLSSAALDDANWDGVDLRGTNLHEATLDGASLARANASGAQLTR
ARARRADLTQADLTHADARCSNLHGASLRRARLGGTQLQSSNLYGADCYGTALARPQLDGANIERTLLAVPGRPELAASR
*

  Nucleotide sequence of regulator: 2643 bp    .

>REG0133 NC_006351:2052905-2055547 [Burkholderia pseudomallei K96243]
ATGAAAATCGTCAAACCCGAAACGCTCGCGCTGATGTGCCGCACGCTGCGCATCGAACGCGCCGACCGGCTGTCGATCGG
CGCGCTCGCCTGCTTCGCGCTGCGCGCGCACGCGCCCGACGGCCCCGGCGATCTCGCGCCGGAGGCCACGCTCTGGCAGA
TCGCCCAGCAATGGCTCGGCGCCCATGCGCCGCTCGACGAGGGCTGGCCGAAGCCGGCGGGCGAATTTCTCGTCTACGGC
GACGCATGCGCGCCGGCGGGCCGCGAGCACGCGGGCGGCGCGCCGTTCGCGGTGCGCGCGCGCATCGGCGCGGCATGCAA
GGCGCGGCTCGTCGATGCGCGCGACCCCGCCGACCGGGTACTCGCCGATTTTCGCGCGCTGCCGCCGTCGCATCCGCAGC
GCGTGCGCGATCTCGGGCCGTTCGACGCGCGCTGGCTGGCCGAGCGGTGGCCTCACCTGCCTTCGGGCACGCGCGCCGAG
CATTTCCATACCGCACCCCGCGATCAGCGGATCGCCGGGTTCTGGCGCGGCGACGAGGACATCGAGCTCGTCAACCTGCA
CGCGCAGCATCCGATCGTCGCCGGCGCGTTGCCGCGCGTGCGGGCGCGCTGCTTCGTCGAGCGCTCGGCCGGCGGCGCGA
CGCGCGTCGACGCGTGCCCGATGCGCGCGGAAACCGTCTGGCTGTTCCCCGGCGCGGCATGCGGCATCGTCCTATACCGC
GGGCTCGCCACGATCGACGACGAAGACGGCGACGACGTCTTGCGCGTGATCGCCGGCTGGGAAGATGCCGCCGCGCCGCC
GTTGCCCGCCGACGCTTATCTCGGCCGGCCGGCCTCCGGGGGCGCCGGCTCGCGCCCGACGCCCGCGCCCGACGCCGCGC
CCGTGGCGTCTGTGGTGCCCGCCGCGCTCACCGCCGAAGAAGCGCACGCCGACGAACATGCGCCGGGCGGATCGGCATCG
GCCTCGCAGGCGCACTCGCCGGCAGCGCCCGAGTTCCCCGAAGCACCGCACGCGCCGGATCTGTCCGCGCTCGAACGGGA
AGCGGCGGCGCTTGCCGGGCAAACCGACGCGTTGCTCGCCGGGCTGGGCATCACCGAAGCGGACATCGCGCGCTTGCTGC
CCGCGCGCGACGCCCCCGCCGAGCTGAATCTGGATCAGCTCGCCACGCTCGCGGCCGAACTCGACGCGCAAACCGCACAG
TGGCAGGCGCAGCAAGCGGCGGCGGCCGTCGAGCGCGGCGACGCGGCCTCGGCGACGCCCGCCGCGCCGGCCACGCCGGA
TGCCGAGGCCGCGCACGAAGCGTCGCTGGCCGACCTGCTTCGGCAGGCCGACGCGCAAATGCGCGCCCTCGTCGAGCAGC
ACGGCCTGTCGCGCGCACGAATGGAGGCGGCCGCGCGAACCCTGCCGGAGCTCGCGCCCCTTGCGGGCTCGCTCGATGCC
CTCGACGCACTCGATGCGCCGCTCGACGTCGATGCCTTGACGGCAGGGCTCGCCGCCGCCGGCGGCGACGCGGCGGCCGA
ACCGGATACGCCGGCCGAACCGAGCCCGCCGGCGCCCGCGAACGAATTCGCCGCCGCCGTGCCCGCCCCCGCATCGTCCA
CCGCCGCGCCGCCGGTGGACGATGCGCCGCCAGGGCCGCTCACGCGCGAGCAAGTAATCGAGCGCCACGCGCGCGGGCTC
GGCTTCGCCGGCCTCGACCTGAGCGGCCTGGACCTGTCGTCGGCCGCGCTCGAGCGCGCGGACTTTCGCCGCGCACGCCT
CGAACGCACCCGCTTCGCGGGCTGCCGGCTCGCCGGCGCATCGTTCGAGCGCGCGCTGCTGTCGCACGCCGATTTCTCGA
ACGCGGACCTGCGCGACGCGGTCTTCGCCGGCGCCTCCGCGCCCGGCGCATCGTGGCGCGGCGCCGTGCTCGAGCGCGCG
CGCCTCGAGCACGGCGACTTCAGCGGCGGCGACTTCGCGCAAGCGTCGCTCGCCGACAGCCATTGCGCGCACGCGCAGTT
CGACGCGAGCGCGATGACGGCGCTCGTCGCGGCGCGCATCGACGGCACGCACGCGAGCTTCGCCGGCTGCACGCTCGACG
CCGCCGATTTCACGTCGGCGCGCCTGCCGCGCGCGAATTTCCAGCATGCGACGCTCGCGGACGCGGCGCTCGCCTGCGCG
CACTGCGACGGCGCCGAATGGTACGGCGCGCAGGCGCCGCGTGCCCGGCTTCGCGCGGCGTCGCTGCGCGGCTCGCGCGC
GGACGCGTCGACGTCGTTCCGGCAAGCCGATCTGAGCAGCGCCGCGCTCGACGACGCGAACTGGGACGGCGTCGACCTGC
GCGGCACGAACCTGCACGAGGCGACGCTCGACGGCGCGAGCCTCGCGCGCGCGAACGCGAGCGGCGCGCAACTGACGCGC
GCGCGCGCACGGCGCGCGGATCTGACCCAGGCCGACCTCACGCACGCGGATGCGCGCTGCTCGAACCTGCACGGCGCATC
GCTGCGCCGCGCACGGCTCGGCGGCACGCAACTGCAATCGAGCAACCTGTACGGCGCCGACTGCTACGGCACCGCGCTCG
CCCGGCCGCAGCTCGACGGCGCGAATATCGAGCGCACGCTCCTCGCCGTGCCGGGCCGCCCCGAACTCGCCGCCTCCCGC
TGA