Detailed information of regulator
Summary
| Regulator ID | REG0089  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Unknown |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00028  (Type i3) |
| Strain | Xanthomonas citri pv. citri str. 306 |
| Replicon | chromosome |
| Sequence | Protein sequence (176 a.a.); Nucleotide sequence (528 bp) |
| Description | EcfK/RpoE are required and essential for T6SS-dependent resistance to Dictyostelium predation,and EcfK acts downstream of PknS. |
| Reference |
External database links
| Locus tag (Gene) | xac4129 (ecfK/RpoE,ECF σ factor) |
| Coordinates (Strand) | 4850278..4850805 (+) |
| NCBI ID | 489582896 |
| RefSeq | NC_003919 |
| Uniprot ID | H8FHB2_XANCI, Q8PF56_XANAC, A0A1T1NQ25_9XANT |
| KEGG ID | xac:XAC4129 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Sigma70_r4_2 | PF08281.14 | 3.1e-16 | 111..163 |
| Sigma70_r2 | PF04542.16 | 1.8e-11 | 12..78 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-175 | VSAHPKRAWARLFTEHGPVLRGFFVRRGANEDAEDLVQETYLRLLRAHQQPGQAIANPEAYLYTVAVNLAREQAARRRQAPLRIEDMEQFAQRLVAGDDVEDSAQRQQRQQRLQTVLAGLPERVRAVLVMQYRDGLSYKQIAERLGVSSHMVKKYVVRGLSACRSALADEGAQWW |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9941 | - | - |
Protein sequence of regulator: 176 a.a. .
>REG0089 NC_003919:4850278-4850805 [Xanthomonas citri pv. citri str. 306]
VSAHPKRAWARLFTEHGPVLRGFFVRRGANEDAEDLVQETYLRLLRAHQQPGQAIANPEAYLYTVAVNLAREQAARRRQA
PLRIEDMEQFAQRLVAGDDVEDSAQRQQRQQRLQTVLAGLPERVRAVLVMQYRDGLSYKQIAERLGVSSHMVKKYVVRGL
SACRSALADEGAQWW*
VSAHPKRAWARLFTEHGPVLRGFFVRRGANEDAEDLVQETYLRLLRAHQQPGQAIANPEAYLYTVAVNLAREQAARRRQA
PLRIEDMEQFAQRLVAGDDVEDSAQRQQRQQRLQTVLAGLPERVRAVLVMQYRDGLSYKQIAERLGVSSHMVKKYVVRGL
SACRSALADEGAQWW*
Nucleotide sequence of regulator: 528 bp .
>REG0089 NC_003919:4850278-4850805 [Xanthomonas citri pv. citri str. 306]
GTGTCGGCACATCCAAAGCGCGCCTGGGCACGTCTGTTCACCGAGCATGGCCCGGTGCTGCGCGGCTTCTTCGTGCGCCG
CGGAGCAAACGAGGACGCCGAAGACCTGGTCCAGGAAACCTATCTGCGCCTGCTGCGTGCGCATCAGCAGCCGGGCCAGG
CCATCGCCAACCCGGAAGCGTATCTCTATACCGTGGCGGTCAATCTGGCGCGCGAGCAGGCGGCGCGGCGCAGGCAGGCC
CCGCTGCGCATCGAGGACATGGAACAGTTCGCCCAACGGCTGGTGGCCGGTGACGATGTCGAAGACAGCGCCCAGCGGCA
ACAGCGCCAGCAACGGCTGCAGACCGTACTGGCCGGGCTGCCCGAGCGCGTGCGTGCGGTGCTGGTGATGCAGTACCGCG
ACGGCCTGAGCTACAAACAGATCGCCGAACGTCTGGGTGTGTCCTCGCACATGGTCAAGAAATACGTGGTGCGCGGCCTG
TCGGCCTGTCGATCGGCGCTGGCCGACGAGGGAGCGCAGTGGTGGTGA
GTGTCGGCACATCCAAAGCGCGCCTGGGCACGTCTGTTCACCGAGCATGGCCCGGTGCTGCGCGGCTTCTTCGTGCGCCG
CGGAGCAAACGAGGACGCCGAAGACCTGGTCCAGGAAACCTATCTGCGCCTGCTGCGTGCGCATCAGCAGCCGGGCCAGG
CCATCGCCAACCCGGAAGCGTATCTCTATACCGTGGCGGTCAATCTGGCGCGCGAGCAGGCGGCGCGGCGCAGGCAGGCC
CCGCTGCGCATCGAGGACATGGAACAGTTCGCCCAACGGCTGGTGGCCGGTGACGATGTCGAAGACAGCGCCCAGCGGCA
ACAGCGCCAGCAACGGCTGCAGACCGTACTGGCCGGGCTGCCCGAGCGCGTGCGTGCGGTGCTGGTGATGCAGTACCGCG
ACGGCCTGAGCTACAAACAGATCGCCGAACGTCTGGGTGTGTCCTCGCACATGGTCAAGAAATACGTGGTGCGCGGCCTG
TCGGCCTGTCGATCGGCGCTGGCCGACGAGGGAGCGCAGTGGTGGTGA